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Reaction Conditions for Restriction Enzymes:

• General Protocol for DNA Digestion
• Five Buffer System
• Dilution of Restriction Enzymes
• Stability During Prolonged Incubation (see below)
• Inactivation
• Considerations for Partial Digestion of DNA
• Chart: Reaction Conditions for Restriction Enzymes

Stability During Prolonged Incubation

The stability of restriction enzymes in a reaction mixture depends upon the nature of the enzyme, the buffer composition and the incubation temperature.
If a restriction enzyme retains its activity in the reaction mixture for more than one hour, DNA can be digested with less enzyme, by using a prolonged incubation period. For exact quantities of enzymes sufficient for overnight digestion, refer to the table "Reaction Conditions for Restriction Enzymes".

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Updated lapkričio 08, 2006 11:06