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Fermentas Conventional Restriction Enzymes & Cross-reference to FastDigest® Enzymes

Note
List of commercial restriction enzymes and their isoschizomers see.

Single letter code:
R = G or A; 
Y = C or T; 
W = A or T; 
M = A or C; 		 K = G or T; 
S = C or G;
H = A, C or T;
V = A, C or G;		 B = C, G or T;
D = A, G or T;
N = G, A, T or C.

AB    CDEF    GHKLMNO    PRS   TVX   Nicking enzyme   Homing Enzyme

Conventional
Enzyme		 Catalog # FastDigest®
Enzyme		 Catalog # Prototype Specificity
5'=>3'
AanI (PsiI)  ER2061 FastDigest® PsiI (AanI) FD2064 PsiI TTA^TAA
AarI ER1581/2     AarI CACCTGC(4/8)^
AasI ER1721/2 FastDigest® DrdI (AasI) FD1724 DrdI GACNNNN^NNGTC
AatII  ER0991/2     AatII  GACGT^C
Acc65I  ER0901/2 FastDigest® Acc65I FD0904 KpnI (GGTAC^C) G^GTACC
AdeI  ER1231/2 FastDigest® DraIII (AdeI) FD1234 DraIII  CACNNN^GTG
AjiI  ER1941     BtrI  CAC^GTC
AjuI  ER1951 FastDigest® AjuI FD1954 AjuI  ^(7/12)GAA(N)7TTGG(11/6)^
AlfI  ER1801     AlfI  ^(10/12)GCA(N)6TGC(12/10)^
AloI  ER1491/2     AloI  ^(7/12-13)GAAC(N)6TCC(12-13/7)^
AluI  ER0011/2     AluI  AG^CT
Alw21I  ER0021/2 FastDigest® Alw21I FD0024 HgiAI  GWGCW^C
Alw26I  ER0031/2 FastDigest® Alw26I FD0034 BsmAI  GTCTC(1/5)^
Alw44I  ER0041/2 FastDigest® ApaLI (Alw44I) FD0044 ApaLI  G^TGCAC
ApaI  ER1411/2/5 FastDigest® ApaI FD1414 ApaI  GGGCC^C
BamHI  ER0051/2/3/5 FastDigest® BamHI FD0054 BamHI  G^GATCC
BauI  ER1841     BsiI  CACGAG(-5/-1)^
BclI  ER0721/2/5 FastDigest® BclI FD0724 BclI  T^GATCA
BcnI  ER0061/2 FastDigest® NciI (BcnI) FD0064 CauII  CC^SGG
BcuI  ER1251/2 FastDigest® SpeI (BcuI) FD1253/4 SpeI  A^CTAGT
BdaI  ER1961     BdaI  ^(10/12)TGA(N)6TCA(12/10)^
BfiI ER1591/2     BfiI ACTGGG(5/4)^
BfmI ER1161/2 FastDigest® SfcI (BfmI) FD1164 SfeI  C^TRYAG
BfuI  ER1501/2     BciVI  GTATCC(6/5)^
BglI  ER0071/2 FastDigest® BglI FD0071/2 BglI  GCCNNNN^NGGC 
BglII  ER0081/2 FastDigest® BglII FD0083/4 BglII  A^GATCT
Bme1390I  ER1421/2 FastDigest® ScrFI (Bme1390I) FD1424 ScrFI  CC^NGG
BoxI  ER1431 FastDigest® PshAI (BoxI) FD1434 PshAI  GACNN^NNGTC
BpiI  ER1011/2 FastDigest® BbsI (BpiI) FD1014 BbvII  GAAGAC(2/6)^
BplI  ER1311/2     BplI  ^(8/13)GAG(N)5CTC(13/8)^
Bpu10I  ER1181/2     Bpu10I  CCTNAGC(-5/-2)^
Bpu1102I  ER0091/2 FastDigest® BlpI (Bpu1102I) FD0094 EspI  GC^TNAGC
BseDI  ER1081/2 FastDigest® BsaJI (BseDI) FD1084 SecI  C^CNNGG
BseGI  ER0871/2 FastDigest® BseGI FD0874 FokI (GGATG(9/13)^) GGATG(2/0)^
BseJI ER1711     BsaBI GATNN^NNATC
BseLI  ER1201/2 FastDigest® BslI (BseLI) FD1204 BsiYI  CCNNNNN^NNGG
BseMI  ER1261/2     BsrDI  GCAATG(2/0)^
BseMII  ER1401/2     BseMII  CTCAG(10/8)^
BseNI  ER0881/2 FastDigest® BseNI FD0884 BsrI  ACTGG(1/-1)^
BseSI  ER1441/2 FastDigest® Bme1580I (BseSI) FD1444 BseSI  GKGCM^C
BseXI  ER1451/2     BbvI  GCAGC(8/12)^
Bsh1236I  ER0921/2 FastDigest® Bsh1236I FD0924 FnuDII  CG^CG
Bsh1285I  ER0891     McrI  CGRY^CG
BshNI  ER1001 FastDigest® BanI (BshNI) FD1004 HgiCI  G^GYRCC
BshTI  ER1461/2 FastDigest® AgeI (BshTI) FD1464 AgeI  A^CCGGT
Bsp68I  ER0111/2     NruI  TCG^CGA
Bsp119I  ER0121 FastDigest® Bsp119I FD0124 AsuII  TT^CGAA
Bsp120I  ER0131/2/3 FastDigest® Bsp120I FD0134 ApaI (GGGCC^C)  G^GGCCC
Bsp143I  ER0781/2     MboI  ^GATC
Bsp1407I  ER0931/2 FastDigest® Bsp1407I FD0933/4 Bsp1407I  T^GTACA
BspLI  ER1151/2 FastDigest® NlaIV (BspLI) FD1154 NlaIV  GGN^NCC
BspOI (BmtI) ER2041/2 FastDigest® BmtI (BspOI) FD2044 BmtI GCTAG^C
BspPI  ER1321/2     BinI  GGATC(4/5)^
BspTI  ER0831/2 FastDigest® AflII (BspTI) FD0834 AflII  C^TTAAG
Bst1107I  ER0701/2 FastDigest® BstZ17I (Bst1107I) FD0704 SnaI  GTA^TAC
BstXI  ER1021/2  FastDigest® BstXI FD1024  BstXI  CCANNNNN^NTGG
Bsu15I  ER0141/2/5 FastDigest® ClaI (Bsu15I) FD0143/4 ClaI  AT^CGAT
BsuRI  ER0151/2 FastDigest® HaeIII (BsuRI) FD0154 HaeIII  GG^CC
BveI  ER1741     BspMI  ACCTGC(4/8)^

CDEF

Conventional
Enzyme		 Catalog # FastDigest®
Enzyme		 Catalog # Prototype Specificity
5'=>3'
CaiI  ER1391/2 FastDigest® AlwNI (CaiI) FD1394 AlwNI  CAGNNN^CTG
CfrI  ER0161/2     CfrI  Y^GGCCR
Cfr9I  ER0171/2     SmaI (CCC^GGG) C^CCGGG
Cfr10I  ER0181/2 FastDigest® BsrFI (Cfr10I) FD0184 Cfr10I  R^CCGGY
Cfr13I  ER0191/2 FastDigest® Sau96I (Cfr13I) FD0194 AsuI  G^GNCC
Cfr42I  ER0201/2/5     SacII  CCGC^GG
CpoI  ER0741/2     RsrII  CG^GWCCG
CseI ER1901/2     HgaI GACGC(5/10)^
Csp6I  ER0211 FastDigest® Csp6I FD0214 RsaI (GT^AC) G^TAC
DpnI  ER1701/2/5 FastDigest® DpnI FD1703/4 DpnI  Gm6A^TC
DraI  ER0221/2/3 FastDigest® DraI FD0224 AhaIII  TTT^AAA
Eam1104I  ER0231/2 FastDigest® EarI (Eam1104I) FD0234 Ksp632I  CTCTTC(1/4)^
Eam1105I  ER0241/2 FastDigest® Eam1105I FD0244 Eam1105I  GACNNN^NNGTC
Ecl136II  ER0251 FastDigest® Ecl136II FD0254 SacI (GAGCT^C)  GAG^CTC
Eco24I  ER0281     HgiJII  GRGCY^C
Eco31I  ER0291/2 FastDigest® Eco31I FD0293/4 Eco31I  GGTCTC(1/5)^
Eco32I  ER0301/2/3/5 FastDigest® EcoRV (Eco32I) FD0303/4 EcoRV  GAT^ATC
Eco47I  ER0311/2 FastDigest® AvaII (Eco47I) FD0314 AvaII  G^GWCC
Eco47III  ER0321/2 FastDigest® AfeI (Eco47III) FD0324 Eco47III  AGC^GCT
Eco52I  ER0331/2      XmaIII  C^GGCCG
Eco57I  ER0341/2     Eco57I  CTGAAG(16/14)^
Eco57MI  ER1671     Eco57MI  CTGRAG(16/14)^
Eco72I  ER0361/2     PmaCI  CAC^GTG
Eco81I  ER0371/2 FastDigest® Bsu36I (Eco81I) FD0374 SauI  CC^TNAGG
Eco88I  ER0381 FastDigest® AvaI (Eco88I) FD0384 AvaI  C^YCGRG
Eco91I  ER0391/2 FastDigest® Eco91I FD0394 BstEII  G^GTNACC
Eco105I  ER0401/2 FastDigest® SnaBI (Eco105I) FD0404 SnaBI  TAC^GTA
Eco130I  ER0411 FastDigest® StyI (Eco130I) FD0414 StyI  C^CWWGG
Eco147I  ER0421/2 FastDigest® StuI (Eco147I) FD0424 StuI  AGG^CCT
EcoO109I  ER0261     DraII  RG^GNCCY
EcoRI  ER0271/2/3/5 FastDigest® EcoRI FD0274 EcoRI  G^AATTC 
EcoRII ER1921/2     EcoRII ^CCWGG
EheI  ER0441 FastDigest® EheI FD0443/4 NarI (GG^CGCC) GGC^GCC
Esp3I  ER0451/2 FastDigest® BsmBI (Esp3I) FD0454 Esp3I  CGTCTC(1/5)^
FaqI  ER1811     FinI  GGGAC(10/14)^
FspAI  ER1661/2 FastDigest® FspAI FD1664 FspAI  RTGC^GCAY
FspBI  ER1761/2 FastDigest® BfaI (FspBI) FD1764 MaeI  C^TAG

GHKLMNO

Conventional
Enzyme		 Catalog # FastDigest®
Enzyme		 Catalog # Prototype Specificity
5'=>3'
GsuI  ER0461/2     GsuI  CTGGAG(16/14)^
HhaI  ER1851 FastDigest® HhaI FD1854 HhaI  GCG^C
Hin1I  ER0471/2 FastDigest® BsaHI (Hin1I) FD0474 AcyI  GR^CGYC
Hin1II  ER1831 FastDigest® NlaIII (Hin1II) FD1834 NlaIII  CATG^
Hin4I ER1601/2     Hin4I ^(8/13-14)GAY(N)5VTC(13-14/8)^
Hin6I  ER0481/2 FastDigest® HinP1I (Hin6I) FD0484 HhaI (GCG^C) G^CGC
HincII  ER0491/2 FastDigest® HincII FD0494 HindII  GTY^RAC
HindIII  ER0501/2/3/5 FastDigest® HindIII FD0504 HindIII  A^AGCTT
HinfI  ER0801/2/3 FastDigest® HinfI FD0804 HinfI  G^ANTC
HpaII  ER0511/2 FastDigest® HpaII FD0514 HpaII  C^CGG
HphI  ER1101/2     HphI  GGTGA(8/7)^
Hpy8I  ER1571/2 FastDigest® Hpy8I FD1574 MjaIV  GTN^NAC
HpyF3I ER1881/2 FastDigest® DdeI (HpyF3I) FD1884 DdeI C^TNAG
HpyF10VI ER1731/2 FastDigest® HpyF10VI FD1734 MwoI GCNNNNN^NNGC
KpnI  ER0521/2/3 FastDigest® KpnI FD0524 KpnI  GGTAC^C
Kpn2I  ER0531/2 FastDigest® Kpn2I FD0534 BspMII  T^CCGGA
KspAI  ER1031/2 FastDigest® HpaI (KspAI) FD1034 HpaI  GTT^AAC
LguI ER1931/2 FastDigest® SapI (LguI) FD1934 SapI GCTCTTC(1/4)^
Lsp1109I (BbvI) ER2071 FastDigest® BbvI (Lsp1109I) FD2074 BbvI GCAGC(8/12)^
LweI ER1621/2     SfaNI GCATC(5/9)^
MauBI  ER2081     MauBI  CG^CGCGCG
MbiI  ER1271     BsrBI  GAGCGG(-3/-3)^
MboI  ER0811/2 FastDigest® MboI   MboI  ^GATC
MboII  ER0821/2 FastDigest® MboII FD0824 MboII  GAAGA(8/7)^ 
MlsI  ER1211/2 FastDigest® MscI (MlsI) FD1214 BalI  TGG^CCA
MluI  ER0561/2 FastDigest® MluI FD0564 MluI  A^CGCGT
MnlI  ER1071/2 FastDigest® MnlI FD1074 MnlI  CCTC(7/6)^
Mph1103I  ER0731/2 FastDigest® NsiI (Mph1103I) FD0734 AvaIII  ATGCA^T
MreI (Sse232I) ER2021/2 FastDigest® MreI FD2024 Sse232I CG^CCGGCG
MspI  ER0541/2 FastDigest® MspI FD0544 HpaII  C^CGG
MssI  ER1341/2 FastDigest® MssI FD1344 PmeI  GTTT^AAAC
MunI  ER0751/2 FastDigest® MfeI (MunI) FD0753/4 MfeI  C^AATTG
MvaI  ER0551/2 FastDigest® MvaI FD0554 EcoRII (^CCWGG) CC^WGG
Mva1269I  ER0961/2 FastDigest® Mva1269I FD0964 BsmI  GAATGC(1/-1)^
NcoI  ER0571/2/5 FastDigest® NcoI FD0573/4 NcoI  C^CATGG
NdeI  ER0581/2/5 FastDigest® NdeI FD0583/4 NdeI  CA^TATG
NheI  ER0971/2/5 FastDigest® NheI FD0973/4 NheI  G^CTAGC
NmuCI  ER1511/2 FastDigest® NmuCI FD1514 Tsp45I  ^GTSAC
NotI  ER0591/2/3/5 FastDigest® NotI FD0593/4 NotI  GC^GGCCGC
NsbI  ER1221/2 FastDigest® FspBI (NsbI) FD1224 MstI  TGC^GCA
OliI ER1631/2     OliI CACNN^NNGTG

PRS

Conventional
Enzyme		 Catalog # FastDigest®
Enzyme		 Catalog # Prototype Specificity
5'=>3'
PaeI  ER0601/2 FastDigest® SphI (PaeI) FD0604 SphI  GCATG^C
PagI  ER1281/2 FastDigest® BspHI (PagI) FD1284 BspHI  T^CATGA
PasI  ER1861     PasI  CC^CWGGG
PauI  ER1091/2     BsePI  G^CGCGC
PdiI  ER1521/2 FastDigest® NaeI (PdiI) FD1524 NaeI  GCC^GGC
PdmI  ER1531/2 FastDigest® PdmI FD1534 XmnI  GAANN^NNTTC
Pfl23II  ER0851     SplI  C^GTACG
PfeI  ER1781     TfiI  G^AWTC
PfoI  ER1751 FastDigest® PfoI FD1754 PfoI  T^CCNGGA
PpiI  ER1541/2     PpiI  ^(7/12)GAAC(N)5CTC(13/8)^
Ppu21I ER1971 FastDigest® BsaAI (Ppu21I) FD1974 BsaAI YAC^GTR
PscI ER1871/2     BspLU11I A^CATGT
Psp5II  ER0761 FastDigest® PpuMI (Psp5II) FD0764 PpuMI  RG^GWCCY
Psp1406I  ER0941/2 FastDigest® AclI (Psp1406I) FD0944 AclI  AA^CGTT
PstI  ER0611/2/3/5 FastDigest® PstI FD0614 PstI  CTGCA^G
PsuI  ER1551 FastDigest® PsuI FD1554 XhoII  R^GATCY
PsyI  ER1331     Tth111I  GACN^NNGTC
PvuI  ER0621/2 FastDigest® PvuI FD0624 PvuI  CGAT^CG
PvuII  ER0631/2/3/5 FastDigest® PvuII FD0634 PvuII  CAG^CTG
RsaI  ER1121/2 FastDigest® RsaI FD1124 RsaI  GT^AC
RseI  ER2001/2 FastDigest® MslI (RseI) FD2004 MslI  CAYNN^NNRTG
SacI  ER1131/2/3/5 FastDigest® SacI FD1133/4 SacI  GAGCT^C
SalI  ER0641/2/3/5 FastDigest® SalI FD0644 SalI  G^TCGAC
SatI ER1641/2 FastDigest® Fnu4HI (SatI) FD1644 Fnu4HI GC^NGC
ScaI  ER0431/2 FastDigest® ScaI FD0434 ScaI  AGT^ACT
SchI  ER1371     PleI (GAGTC(4/5)^) GAGTC(5/5)^
SdaI  ER1191/2 FastDigest® SbfI (SdaI) FD1194 Sse8387I CCTGCA^GG
SfaAI (AsiSI)  ER2091 FastDigest® AsiSI (SfaAI) FD2094 SgfI GCGAT^CGC
SduI  ER0651 FastDigest® Bsp1286I (SduI) FD0654 SduI  GDGCH^C
SfiI  ER1821     SfiI  GGCCNNNN^NGGCC
SgrDI ER2031       CG^TCGACG
SgsI ER1891/2 FastDigest® AscI (SgsI) FD1894 AscI GG^CGCGCC
SmaI  ER0661/2/3/5 FastDigest® SmaI FD0663/4 SmaI  CCC^GGG
SmiI  ER1241/2 FastDigest® SwaI (SmiI) FD1244 SwaI  ATTT^AAAT
SmoI  ER1981     SmlI  C^TYRAG
SmuI  ER1691/2     FauI  CCCGC(4/6)^
SsiI  ER1791 FastDigest® AciI (SsiI) FD1794 AciI  C^CGC
SspI  ER0771/2 FastDigest® SspI FD0774 SspI  AAT^ATT 

TVX

Conventional
Enzyme		 Catalog # FastDigest®
Enzyme		 Catalog # Prototype Specificity
5'=>3'
TaaI  ER1361/2 FastDigest® TaaI FD1364 Tsp4CI  ACN^GT
TaiI  ER1141/2 FastDigest® TaiI FD1144 MaeII (A^CGT) ACGT^
TaqI  ER0671/2/3 FastDigest® TaqI FD0674 TaqI  T^CGA
TasI  ER1351/2     TspEI  ^AATT
TatI  ER1291/2     TatI  W^GTACW
TauI ER1651/2     TauI GCSG^C
Tru1I  ER0981/2/3 FastDigest® Tru1I FD0984 MseI  T^TAA
TscAI (TspRI)  ER2101 FastDigest® TspRI (TscAI) FD2104 TspRI  CASTG(2/-7)^C
TsoI ER1991     TsoI TARCCA(11/9)^
TstI ER1911     TstI ^(8/13)CAC(N)6TCC(12/7)^
Van91I  ER0711/2 FastDigest® PflMI (Van91I) FD0714 PflMI  CCANNNN^NTGG
VspI  ER0911/2 FastDigest® AseI (VspI) FD0914 VspI  AT^TAAT
XagI  ER1301/2 FastDigest® EcoNI (XagI) FD1304 EcoNI  CCTNN^NNNAGG
XapI  ER1381/2 FastDigest® XapI FD1383/4 ApoI  R^AATTY
XbaI  ER0681/2/3/5 FastDigest® XbaI FD0684 XbaI  T^CTAGA
XceI  ER1471/2     NspI  RCATG^Y
XhoI  ER0691/2/3/5 FastDigest® XhoI FD0694 XhoI  C^TCGAG
XmaJI  ER1561/2 FastDigest® AvrII (XmaJI) FD1564 AvrII  C^CTAGG
XmiI  ER1481/2 FastDigest® AccI (XmiI) FD1484 AccI  GT^MKAC

Nicking and homing enzymes

Fermentas
Enzyme		 Prototype Specificity
5'=>3'		 Catalog #
Nb.Bpu10I  Nb.Bpu10I  GC^TNAGG
CG  ANTCC		 ER1681
Nb.Mva1269I Nb.BsmI G^CATTC
C  GTAAG		 ER2051
Nt.Bpu10I  Nt.Bpu10I  GC  TNAGG
CG^ANTCC		 ER2011
I-SceI  I-SceI  TAGGG ATAA^CAGGGTAAT
ATCCC^TATT GTCCCATTA		 ER1771

Single letter code:
R = G or A; 
Y = C or T; 
W = A or T; 
M = A or C; 		 K = G or T; 
S = C or G;
H = A, C or T;
V = A, C or G;		 B = C, G or T;
D = A, G or T;
N = G, A, T or C.
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Updated gegužės 07, 2008 14:06