Alphabetic List of Commercially Available Restriction Enzymes (P--Z)
A number of restriction enzymes discovered by Fermentas are isoschizomers of commonly used prototype restriction enzymes. The following table will help you find the appropriate Fermentas enzymes for your experiments.
A B C-D-E-F-G H-I-K-L-M-N-O P-R-S-T-V-X-Z
Note
- Enzymes in parentheses have different cleavage specificities.
- Isoschizomers with different sensitivity to methylation are indicated by "m".
- DpnI requires the presence of N6-methyladenine within the recognition sequence GATC.
- Enzymes produced by Fermentas appear in green.
Single letter code: R = G or A;
Y = C or T;
W = A or T;
M = A or C;K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;B = C, G or T;
D = A, G or T;
N = G, A, T or C.
Enzyme Specificity 5'=>3' Fermentas enzyme Commercially available isoschizomers PacI TTAAT^TAA PaeI,
FastDigest® SphI (PaeI)GCATG^C PaeI, FastDigest® SphI (PaeI) BbuI, SphI PaeR7I C^TCGAG XhoI, FastDigest® XhoI Sfr274I, SlaI, StrI, TliI, XhoI, FastDigest® XhoI PagI T^CATGA PagI BspHIm, RcaI PalAI GG^CGCGCC SgsI, FastDigest® AscI (SgsI) AscI, SgsI, FastDigest® AscI (SgsI) PasI CC^CWGGG PasI PauI G^CGCGC PauI BsePI, BssHII PceI AGG^CCT Eco147I, FastDigest® StuI (Eco147I) AatI, Eco147I, FastDigest® StuI (Eco147I), SseBI, StuI PciI A^CATGT PscI BspLU11I, PscI PciSI GCTCTTC(1/4)^ LguI, FastDigest® SapI (LguI) LguI, FastDigest® SapI (LguI), SapI PctI GAATGC(1/-1)^ Mva1269I, FastDigest® Mva1269I BsaMI, BsmI, Mva1269I, FastDigest® Mva1269I PdiI GCC^GGC PdiI (MroNI), NaeI, (NgoMIV) PdmI,
FastDigest® PdmIGAANN^NNTTC PdmI, FastDigest® PdmI Asp700I, MroXI, XmnIm PfeI G^AWTC PfeI TfiI Pfl23II C^GTACG Pfl23II BsiWI, PspLI PflFI GACN^NNGTC PsyI AspI, PsyI, Tth111I PflMI CCANNNN^NTGG Van91I, FastDigest® PflMI (Van91I) AccB7I, Van91I, FastDigest® PflMI (Van91I) PfoI,
FastDigest® PfoIT^CCNGGA PfoI, FastDigest® PfoI PhoI GG^CC FastDigest® HaeIII (BsuRI), BsuRI BshFI, BsnI, BspANI, FastDigest® HaeIII (BsuRI), BsuRI, HaeIII PinAI A^CCGGT BshTI, FastDigest® AgeI (BshTI) AgeI, AsiGI, BshTI, FastDigest® AgeI (BshTI), CspAI Ple19I CGAT^CG PvuI, FastDigest® PvuI BpvUI, MvrI, PvuI, FastDigest® PvuI PleI GAGTC(4/5)^ (SchI) (GAGTC(5/5)) (MlyIm), PpsI, (SchI) PmaCI CAC^GTG Eco72I AcvI, BbrPI, Eco72I, PmlI, PspCI PmeI GTTT^AAAC MssIm, FastDigest® MssIm MssIm, FastDigest® MssIm PmlI CAC^GTG Eco72I AcvI, BbrPI, Eco72I, PmaCI, PspCI PpiI ^(7/12)GAAC(N)5CTC(13/8)^ PpiI PpsI GAGTC(4/5)^ (SchI) (GAGTC(5/5)) (MlyI), PleI, (SchI) Ppu21I YAC^GTR Ppu21I BsaAI, BstBAI PpuMI RG^GWCCY Psp5II, FastDigest® PpuMI (Psp5II) Psp5II, FastDigest® PpuMI (Psp5II), PspPPI PscI A^CATGT PscI BspLU11I, PciI PshAI GACNN^NNGTC BoxI, FastDigest® PshAI (BoxI) BoxI, FastDigest® PshAI (BoxI), BstPAI PshBI AT^TAAT VspI, FastDigest® AseI (VspI) AseI, VspI, FastDigest® AseI (VspI) PsiI TTA^TAA Psp124BI GAGCT^C (Ecl136II, FastDigest® Ecl136II) (GAG^CTC), SacI, FastDigest® SacI (Ecl136II), (FastDigest® Ecl136II), (EcoICRI), SacI, FastDigest® SacI, SstI Psp1406I,
FastDigest® AclI (Psp1406I)AA^CGTT Psp1406I, FastDigest® AclI (Psp1406I) AclI Psp5II,
FastDigest® PpuMI (Psp5II)RG^GWCCY Psp5II, FastDigest® PpuMI (Psp5II) PpuMI, PspPPI Psp6I ^CCWGG EcoRII, (MvaI) (CC^WGG), (FastDigest® MvaI)(CC^WGG) AjnI, (BptI), (BseBI), (Bst2UI), (BstNI), (BstOI), EcoRII, (MvaI), (FastDigest® MvaI), PspGI PspCI CAC^GTG Eco72I AcvI, BbrPI, Eco72I, PmaCI, PmlI PspEI G^GTNACC Eco91I, FastDigest® Eco91I BstEII, BstPI, Eco91I, FastDigest® Eco91I, EcoO65I PspGI ^CCWGG EcoRII, (MvaIm) (CC^WGG),
(FastDigest® MvaIm) (CC^WGG)AjnIm, (BptI), (BseBI), (Bst2UIm), (BstNIm), (BstOIm), EcoRII, (MvaIm), (FastDigest® MvaIm), Psp6I PspLI C^GTACG Pfl23II BsiWI, Pfl23II PspN4I GGN^NCC BspLI, FastDigest® NlaIV (BspLI) BmiI, BspLI, FastDigest® NlaIV (BspLI), NlaIV PspOMI G^GGCCC (ApaI) (GGGCC^C), (FastDigest® ApaI)(GGGCC^C), Bsp120I, FastDigest® Bsp120I (ApaI), (FastDigest® ApaI), Bsp120I, FastDigest® Bsp120I PspPPI RG^GWCCY Psp5II, FastDigest® PpuMI (Psp5II) PpuMI, Psp5II, FastDigest® PpuMI (Psp5II) PspXI VC^TCGAGB PsrI ^(7/12)GAAC(N)6TAC(12/7)^ PstI,
FastDigest® PstICTGCA^G PstI, FastDigest® PstI BspMAI PsuI R^GATCY PsuI BstX2I, BstYI, MflIm, XhoII PsyI GACN^NNGTC PsyI AspI, PflFI, Tth111I PvuI,
FastDigest® PvuICGAT^CG PvuI, FastDigest® PvuI BpvUI, MvrI, Ple19I PvuII,
FastDigest® PvuIICAG^CTG PvuII, FastDigest® PvuII RcaI T^CATGA PagI BspHI, PagI RgaI GCGAT^CGC AsiSI, SgfI RsaI,
FastDigest® RsaIGT^AC (Csp6Im, FastDigest® Csp6Im) (G^TAC), RsaI, FastDigest® RsaI AfaI, (Csp6Im), (FastDigest® Csp6Im) RseI,
FastDigest® MslI (RseI)CAYNN^NNRTG RseI, FastDigest® MslI (RseI) SmiMI Rsr2I CG^GWCCG CpoI CpoI, CspI, RsrII RsrII CG^GWCCG CpoI CpoI, CspI, Rsr2I SacI,
FastDigest® SacIGAGCT^C (Ecl136IIm, FastDigest® Ecl136IIm) (GAG^CTC), SacI,
FastDigest® SacI(Ecl136IIm), (FastDigest® Ecl136IIm), (EcoICRI), Psp124BI, SstI SacII CCGC^GG Cfr42I Cfr42I, KspI, Sfr303I, SgrBI, SstII SalI,
FastDigest® SalIG^TCGAC SalI, FastDigest® SalI SanDI GG^GWCCC SapI GCTCTTC(1/4)^ LguI, FastDigest® SapI (LguI) LguI, FastDigest® SapI (LguI), PciSI SatI,
FastDigest® Fnu4HI (SatI)GC^NGC SatI, FastDigest® Fnu4HI (SatI) BisI, Fnu4HI, Fsp4HI, ItaI Sau3AI ^GATC Bsp143Im, (DpnI, FastDigest® DpnI) (GA^TC), MboIm, FastDigest® MboIm BfuCIm, Bsp143Im, BssMI, (BstKTIm), BstMBI, (DpnI), (FastDigest® DpnI), DpnIIm, Kzo9I, (MalI), MboIm, FastDigest® MboIm, NdeIIm Sau96I G^GNCC Cfr13I, FastDigest® Sau96I (Cfr13I) AspS9I, (BmgT120I), Cfr13I, FastDigest® Sau96I (Cfr13I) SbfI CCTGCA^GG SdaI, FastDigest® SbfI (SdaI) SdaI, FastDigest® SbfI (SdaI), Sse8387I ScaI AGT^ACT ScaI AssI, BmcAI, ZrmI SchI GAGTC(5/5)^ SchI MlyI, (PleI), (PpsI) ScrFI CC^NGG Bme1390I, FastDigest® ScrFI (Bme1390I) Bme1390I, FastDigest® ScrFI (Bme1390I), BmrFI, (BssKI), (BstSCI), MspR9I, (StyD4I) SdaI,
FastDigest® SbfI (SdaI)CCTGCA^GG SdaI, FastDigest® SbfI (SdaI) SbfI, Sse8387I SduI GDGCH^C SduI Bsp1286I, MhlI SexAI A^CCWGGT MabIm SfaNI GCATC(5/9)^ LweI LweI SfcI C^TRYAG BfmI, FastDigest® SfcI (BfmI) BfmI, FastDigest® SfcI (BfmI), BpcI, BstSFI SfiI GGCCNNNN^NGGCC SfiI SfoI GGC^GCC FastDigest® EheIm, EheIm (BbeIm), DinI, EgeI, FastDigest® EheIm, EheIm, (KasIm), (Mly113I), (NarIm) Sfr274I C^TCGAG XhoI, FastDigest® XhoI PaeR7I, SlaI, StrI, TliI, XhoI, FastDigest® XhoI Sfr303I CCGC^GG Cfr42I Cfr42I, KspI, SacII, SgrBI, SstII SfuI TT^CGAA Bsp119Im, FastDigest® Bsp119Im AsuII, Bpu14I, Bsp119Im, FastDigest® Bsp119Im, BspT104Im, BstBIm, Csp45I, NspVm SgfI GCGAT^CGC AsiSI, RgaI SgrAI CR^CCGGYG SgrBI CCGC^GG Cfr42I Cfr42I, KspI, SacII, Sfr303I, SstII SgsI,
FastDigest® AscI (SgsI)GG^CGCGCC SgsI, FastDigest® AscI (SgsI) AscI, PalAI SinI G^GWCC Eco47I, FastDigest® AvaII (Eco47I) AvaII, Bme18I, Eco47I, FastDigest® AvaII (Eco47I), VpaK11BI SlaI C^TCGAG XhoI, FastDigest® XhoI PaeR7I, Sfr274I, StrI, TliI, XhoI, FastDigest® XhoI SmaI,
FastDigest® SmaICCC^GGG (Cfr9Im) (C^CCGGG), SmaIm, FastDigest® SmaIm (Cfr9Im), (TspMIm), (XmaCI), (XmaIm) SmiI,
FastDigest® SwaI (SmiI)ATTT^AAAT SmiI, FastDigest® SwaI (SmiI) SwaI SmiMI CAYNN^NNRTG MslI SmlI C^TYRAG SmoI SmoI SmoI C^TYRAG SmoI SmlI SmuI CCCGC(4/6)^ SmuI FauI SnaBI TAC^GTA Eco105I, FastDigest® SnaBI (Eco105I) BstSNI, Eco105I, FastDigest® SnaBI (Eco105I) SpeI A^CTAGT BcuI, FastDigFastDigest® SpeI (BcuI) AhlI, BcuI, FastDigest® SpeI (BcuI) SphI GCATG^C PaeI, FastDigest® SphI (PaeI) BbuI, PaeI, FastDigest® SphI (PaeI) SrfI GCCC^GGGC Sse8387I CCTGCA^GG SdaI, FastDigest® SbfI (SdaI) SbfI, SdaI, FastDigest® SbfI (SdaI) Sse9I ^AATT TasI TasI, Tsp509I, TspEI SseBI AGG^CCT Eco147I, FastDigest® StuI (Eco147I) AatIm, Eco147I, FastDigest® StuI (Eco147I), PceI, StuI SsiI,
FastDigest® AciI (SsiI)CCGC(-3/-1)^ SsiI, FastDigest® AciI (SsiI) AciI, BspACI SspBI T^GTACA Bsp1407I, FastDigest® Bsp1407I Bsp1407I, FastDigest® Bsp1407I, BsrGI, BstAUI SspI,
FastDigest® SspIAAT^ATT SspI, FastDigest® SspI SstI GAGCT^C (Ecl136IIm, FastDigest® Ecl136IIm) (GAG^CTC), SacI,
FastDigest® SacI(Ecl136IIm), (FastDigest® Ecl136IIm), (EcoICRI), Psp124BI, SacI, FastDigest® SacI SstII CCGC^GG Cfr42I Cfr42I, KspI, SacII, Sfr303I, SgrBI StrI C^TCGAG XhoI, FastDigest® XhoI PaeR7I, Sfr274I, SlaI, TliI, XhoI, FastDigest® XhoI StuI AGG^CCT Eco147I, FastDigest® StuI (Eco147I) AatI, Eco147I, FastDigest® StuI (Eco147I), PceI, SseBI StyD4I ^CCNGG (Bme1390I) (CC^NGG),
(FastDigest® ScrFI (Bme1390I)) (CC^NGG)(Bme1390I), (FastDigest® ScrFI (Bme1390I)), (BmrFI), BssKI, BstSCI, (MspR9I), (ScrFI) StyI C^CWWGG Eco130I, FastDigest® StyI (Eco130I) BssT1I, Eco130I, FastDigest® StyI (Eco130I), EcoT14I, ErhI SwaI ATTT^AAAT SmiI, FastDigest® SwaI (SmiI) SmiI, FastDigest® SwaI (SmiI) TaaI,
FastDigest® TaaIACN^GT TaaI, FastDigest® TaaI Bst4CI, HpyCH4III TaiI,
FastDigest® TaiIACGT^ TaiI, FastDigest® TaiI (HpyCH4IVm), (MaeII) TaqI,
FastDigest® TaqIT^CGA TaqI, FastDigest® TaqI TaqII GACCGA(11/9)^ TasI ^AATT TasI Sse9I, Tsp509I, TspEI TatI W^GTACW TatI TauI GCSG^C TauI TfiI G^AWTC PfeI PfeI TliI C^TCGAG XhoI, FastDigest® XhoI PaeR7I, Sfr274I, SlaI, StrI, XhoI, FastDigest® XhoI Tru1I,
FastDigest® Tru1IT^TAA Tru1I, FastDigest® Tru1I MseI, Tru9I Tru9I T^TAA Tru1I, FastDigest® Tru1I MseI, Tru1I, FastDigest® Tru1I TseI G^CWGC ApeKI TsoI TARCCA(11/9)^ TsoI Tsp45I ^GTSAC NmuCI, FastDigest® NmuCI NmuCI, FastDigest® NmuCI Tsp509I ^AATT TasI Sse9I, TasI, TspEI TspDTI ATGAA(11/9)^ TspEI ^AATT TasI Sse9I, TasI, Tsp509I TspGWI ACGGA(11/9)^ TspMI C^CCGGG Cfr9Im, (SmaIm) (CCC^GGG),
(FastDigest® SmaIm) (CCC^GGG)Cfr9Im, (SmaIm), (FastDigest® SmaIm), XmaCI, XmaIm TspRI CASTGNN(0/-7)^ TstI ^(8/13)CAC(N)6TCC(12/7)^ TstI Tth111I GACN^NNGTC PsyI AspI, PflFI, PsyI Van91I,
FastDigest® PflMI (Van91I)CCANNNN^NTGG Van91I, FastDigest® PflMI (Van91I) AccB7I, PflMI Vha464I C^TTAAG BspTI AflII, BfrI, BspTI, Bst98I, MspCI VneI G^TGCAC Alw44I Alw44I, ApaLI VpaK11BI G^GWCC Eco47I, FastDigest® AvaII (Eco47I) AvaII, Bme18I, Eco47I, FastDigest® AvaII (Eco47I), SinI VspI,
FastDigest® AseI (VspI)AT^TAAT VspI, FastDigest® AseI (VspI) AseI, PshBI XagI,
FastDigest® EcoNI (XagI)CCTNN^NNNAGG XagI, FastDigest® EcoNI (XagI) BstENI, EcoNI XapI,
FastDigest® XapIR^AATTY XapI, FastDigest® XapI AcsI, ApoI XbaI,
FastDigest® XbaIT^CTAGA XbaI, FastDigest® XbaI XceI RCATG^Y XceI BstNSI, NspI XcmI CCANNNNN^NNNNTGG XhoI,
FastDigest® XhoIC^TCGAG XhoI, FastDigest® XhoI PaeR7I, Sfr274I, SlaI, StrI, TliI XhoII R^GATCY PsuI BstX2I, BstYI, MflIm, PsuI XmaCI C^CCGGG Cfr9I, (SmaI) (CCC^GGG),
(FastDigest® SmaI) (CCC^GGG)Cfr9I, (SmaI), (FastDigest® SmaI), TspMI, XmaI XmaI C^CCGGG Cfr9Im, (SmaIm) (CCC^GGG),
(FastDigest® SmaIm) (CCC^GGG)Cfr9Im, (SmaIm), (FastDigest® SmaIm), TspMIm, XmaCI XmaJI,
FastDigest® AvrII (XmaJI)C^CTAGG XmaJI, FastDigest® AvrII (XmaJI) AspA2I, AvrII, BlnI XmiI,
FastDigest® AccI (XmiI)GT^MKAC XmiI, FastDigest® AccI (XmiI) AccI, FblI XmnI GAANN^NNTTC PdmIm, FastDigest® PdmIm Asp700I, MroXI, PdmIm, FastDigest® PdmIm XspI C^TAG FspBI, FastDigest® BfaI (FspBI) BfaI, FspBI, FastDigest® BfaI (FspBI), MaeI ZraI GAC^GTC (AatIIm) (GACGT^C) (AatIIm) ZrmI AGT^ACT ScaI AssI, BmcAI, ScaI Zsp2I ATGCA^T FastDigest® NsiI (Mph1103I), Mph1103I EcoT22I, FastDigest® NsiI (Mph1103I), Mph1103I, NsiI
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Updated sausio 31, 2008 09:22
