Alphabetic List of Commercially Available Restriction Enzymes (H--O)
A number of restriction enzymes discovered by Fermentas are isoschizomers of commonly used prototype restriction enzymes. The following table will help you find the appropriate Fermentas enzymes for your experiments.
A B C-D-E-F-G H-I-K-L-M-N-O P-R-S-T-V-X-Z
Note
- Enzymes in parentheses have different cleavage specificities.
- Isoschizomers with different sensitivity to methylation are indicated by "m".
- DpnI requires the presence of N6-methyladenine within the recognition sequence GATC.
- Enzymes produced by Fermentas appear in green.
Single letter code: R = G or A;
Y = C or T;
W = A or T;
M = A or C;K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;B = C, G or T;
D = A, G or T;
N = G, A, T or C.
Enzyme Specificity 5'=>3' Fermentas enzyme Commercially available isoschizomers HaeII RGCGC^Y BstH2I HaeIII GG^CC FastDigest® HaeIII (BsuRI), BsuRI BshFI, BsnI, BspANI, FastDigest® HaeIII (BsuRI), BsuRI, PhoI HapII C^CGG HpaII, FastDigest® HpaII, MspIm, FastDigest® MspIm BsiSIm, HpaII, FastDigest® HpaII, MspIm, FastDigest® MspIm HgaI GACGC(5/10)^ CseI CseI HhaI,
FastDigest® HhaIGCG^C HhaI, FastDigest® HhaI, (Hin6I, FastDigest® HinP1I (Hin6I)) (G^CGC) AspLEI, BstHHI, CfoI, (Hin6I), (FastDigest® HinP1I (Hin6I)), (HinP1I), (HspAI) Hin1I,
FastDigest® BsaHI (Hin1I)GR^CGYC Hin1I, FastDigest® BsaHI (Hin1I) AcyI, BsaHIm, BssNI, BstACI, Hsp92I Hin1II CATG^ Hin1II (CviAII), FaeI, (FatI), Hsp92II, NlaIII Hin4I ^(8/13)GAY(N)5VTC(13/8)^ Hin4I Hin6I,
FastDigest® HinP1I (Hin6I)G^CGC (HhaI) (GCG^C), (FastDigest® HhaI)(GCG^C), Hin6I, FastDigest® HinP1I (Hin6I) (AspLEI), (BstHHI), (CfoI), (HhaI), (FastDigest® HhaI), HinP1I, HspAI HincII,
FastDigest® HincIIGTY^RAC HincII, FastDigest® HincII HindII HindII GTY^RAC HincII, FastDigest® HincII HincII, FastDigest® HincII HindIII,
FastDigest® HindIIIA^AGCTT HindIII, FastDigest® HindIII HinfI,
FastDigest® HinfIG^ANTC HinfIm, FastDigest® HinfIm HinP1I G^CGC (HhaI) (GCG^C), (FastDigest® HhaI)(GCG^C), Hin6I, FastDigest® HinP1I (Hin6I) (AspLEI), (BstHHI), (CfoI), (HhaI), (FastDigest® HhaI), Hin6I, FastDigest® HinP1I (Hin6I), HspAI HpaI GTT^AAC KspAI, FastDigest® HpaI (KspAI) KspAI, FastDigest® HpaI (KspAI) HpaII,
FastDigest® HpaIIC^CGG HpaIIm, FastDigest® HpaIIm, MspIm, FastDigest® MspIm BsiSIm, HapII, MspIm, FastDigest® MspIm HphI GGTGA(8/7)^ HphI AsuHPI Hpy188I TCN^GA Hpy188III TC^NNGA Hpy8I,
FastDigest® Hpy8IGTN^NAC Hpy8I, FastDigest® Hpy8I Hpy99I CGWCG^ HpyCH4III ACN^GT TaaI, FastDigest® TaaI Bst4CI, TaaI, FastDigest® TaaI HpyCH4IV A^CGT (TaiIm, FastDigest® TaiIm) (ACGT^) MaeII, (TaiIm), (FastDigest® TaiIm) HpyCH4V TG^CA HpyF10VI,
FastDigest® HpyF10VIGCNNNNN^NNGC HpyF10VI, FastDigest® HpyF10VI BstMWI, MwoI HpyF3I,
FastDigest® DdeI (HpyF3I)C^TNAG HpyF3I, FastDigest® DdeI (HpyF3I) BstDEI, DdeI Hsp92I GR^CGYC Hin1I, FastDigest® BsaHI (Hin1I) AcyI, BsaHI, BssNI, BstACI, Hin1I, FastDigest® BsaHI (Hin1I) Hsp92II CATG^ Hin1II (CviAII), FaeI, (FatI), Hin1II, NlaIII HspAI G^CGC (HhaI) (GCG^C), (FastDigest® HhaI)(GCG^C), Hin6I, FastDigest® HinP1I (Hin6I) (AspLEI), (BstHHI), (CfoI), (HhaI), (FastDigest® HhaI), Hin6I, FastDigest® HinP1I (Hin6I), HinP1I ItaI GC^NGC SatI, FastDigest® Fnu4HI (SatI) BisI, Fnu4HIm, Fsp4HIm, SatI, FastDigest® Fnu4HI (SatI) KasI G^GCGCC (EheIm, FastDigest® EheIm) (GGC^GCC) (BbeI), (DinI), (EgeI), (EheIm), (FastDigest® EheIm), (Mly113I), (NarIm), (SfoIm) Kpn2I,
FastDigest® Kpn2IT^CCGGA Kpn2I, FastDigest® Kpn2I AccIIIm, Aor13HI, BlfI, BseAIm, Bsp13I, BspEIm, MroI KpnI,
FastDigest® KpnIGGTAC^C (Acc65Im, FastDigest® Acc65Im) (G^GTACC), KpnI, FastDigest® KpnI (Acc65Im), (FastDigest® Acc65Im), (Asp718Im) Ksp22I T^GATCA BclI, FastDigest® BclI BclI, FastDigest® BclI, FbaI Ksp632I CTCTTC(1/4)^ Eam1104I Bst6I, Eam1104I, EarI KspAI,
FastDigest® HpaI (KspAI)GTT^AAC KspAI, FastDigest® HpaI (KspAI) HpaI KspI CCGC^GG Cfr42I Cfr42I, SacII, Sfr303I, SgrBI, SstII Kzo9I ^GATC Bsp143I, (DpnI, FastDigest® DpnI) (GA^TC), MboIm, FastDigest® MboIm BfuCI, Bsp143I, BssMI, (BstKTIm), BstMBI, (DpnI), (FastDigest® DpnI), DpnIIm, (MalI), MboIm, FastDigest® MboIm, NdeIIm, Sau3AI LguI,
FastDigest® SapI (LguI)GCTCTTC(1/4)^ LguI, FastDigest® SapI (LguI) PciSI, SapI LweI GCATC(5/9)^ LweI SfaNI MabI A^CCWGGT SexAIm MaeI C^TAG FspBI, FastDigest® BfaI (FspBI) BfaI, FspBI, FastDigest® BfaI (FspBI), XspI MaeII A^CGT (TaiI, FastDigest® TaiI) (ACGT^) HpyCH4IV, (TaiI), (FastDigest® TaiI) MaeIII ^GTNAC MalI GA^TC (Bsp143I) (^GATC), DpnI, FastDigest® DpnI, (MboI, FastDigest® MboI) (^GATC) (BfuCI), (Bsp143I), (BssMI), (BstKTI), (BstMBI), DpnI, FastDigest® DpnI, (DpnII), (Kzo9I), (MboI), (FastDigest® MboI), (NdeII), (Sau3AI) MamI GATNN^NNATC BseJI BsaBI, Bse8I, BseJI MbiI CCGCTC(-3/-3)^ MbiI AccBSI, BsrBIm MboI,
FastDigest® MboI^GATC Bsp143Im, (DpnI, FastDigest® DpnI) (GA^TC), MboIm, FastDigest® MboIm BfuCIm, Bsp143Im, BssMI, (BstKTI), BstMBI, (DpnI), (FastDigest® DpnI), DpnIIm, Kzo9Im, (MalI), NdeIIm, Sau3AIm MboII,
FastDigest® MboIIGAAGA(8/7)^ MboIIm, FastDigest® MboIIm MfeI C^AATTG MunI, FastDigest® MfeI (MunI) MunI, FastDigest® MfeI (MunI) MflI R^GATCY PsuIm BstX2I, BstYIm, PsuIm, XhoIIm MhlI GDGCH^C SduI Bsp1286I, SduI MlsI,
FastDigest® MscI (MlsI)TGG^CCA MlsI, FastDigest® MscI (MlsI) BalI, MluNI, MscI, Msp20I MluI,
FastDigest® MluIA^CGCGT MluI, FastDigest® MluI MluNI TGG^CCA MlsI, FastDigest® MscI (MlsI) BalI, MlsI, FastDigest® MscI (MlsI), MscI, Msp20I Mly113I GG^CGCC (EheI, FastDigest® EheI) (GGC^GCC) (BbeI), (DinI), (EgeI), (EheI), (FastDigest® EheI), (KasI), NarI, (SfoI) MlyI GAGTC(5/5)^ SchI (PleIm), (PpsI), SchI MmeI TCCRAC(20/18)^ MnlI,
FastDigest® MnlICCTC(7/6)^ MnlI, FastDigest® MnlI Mph1103I,
FastDigest® NsiI (Mph1103I)ATGCA^T FastDigest® NsiI (Mph1103I), Mph1103I EcoT22I, NsiI, Zsp2I MroI T^CCGGA Kpn2I, FastDigest® Kpn2I AccIIIm, Aor13HI, BlfI, BseAIm, Bsp13I, BspEIm, Kpn2I, FastDigest® Kpn2I MroNI G^CCGGC (PdiI) (GCC^GGC) (NaeI), NgoMIV, (PdiI) MroXI GAANN^NNTTC PdmI, FastDigest® PdmI Asp700I, PdmI, FastDigest® PdmI, XmnI MscI TGG^CCA MlsI, FastDigest® MscI (MlsI) BalI, MlsI, FastDigest® MscI (MlsI), MluNI, Msp20I MseI T^TAA Tru1I, FastDigest® Tru1I Tru1I, FastDigest® Tru1I, Tru9I MslI CAYNN^NNRTG RseI, FastDigest® MslI (RseI) SmiMI Msp20I TGG^CCA MlsI, FastDigest® MscI (MlsI) BalI, MlsI, FastDigest® MscI (MlsI), MluNI, MscI MspA1I CMG^CKG MspCI C^TTAAG BspTI AflII, BfrI, BspTI, Bst98I, Vha464I MspI,
FastDigest® MspIC^CGG HpaIIm, FastDigest® HpaIIm, MspI, FastDigest® MspI BsiSI, HapIIm, HpaIIm, FastDigest® HpaIIm MspR9I CC^NGG Bme1390I, FastDigest® ScrFI (Bme1390I) Bme1390I, FastDigest® ScrFI (Bme1390I), BmrFI, (BssKI), (BstSCI), ScrFI, (StyD4I) MssI,
FastDigest® MssIGTTT^AAAC MssI, FastDigest® MssI PmeIm MunI,
FastDigest® MfeI (MunI)C^AATTG MunI, FastDigest® MfeI (MunI) MfeI Mva1269I,
FastDigest® Mva1269IGAATGC(1/-1)^ Mva1269I, FastDigest® Mva1269I BsaMI, BsmI, PctI MvaI,
FastDigest® MvaICC^WGG (EcoRIIm) (^CCWGG), MvaI,
FastDigest® MvaI(AjnI), BptI, BseBI, Bst2UI, BstNI, BstOI, (EcoRIIm), (Psp6I), (PspGIm) MvnI CG^CG Bsh1236I, FastDigest® Bsh1236I AccII, Bsh1236I, FastDigest® Bsh1236I, BstFNI, BstUI MvrI CGAT^CG PvuI, FastDigest® PvuI BpvUI, Ple19I, PvuI, FastDigest® PvuI MwoI GCNNNNN^NNGC HpyF10VI, FastDigest® HpyF10VI BstMWI, HpyF10VI, FastDigest® HpyF10VI NaeI GCC^GGC PdiI (MroNI), (NgoMIV), PdiI NarI GG^CGCC (EheIm, FastDigest® EheIm) (GGC^GCC) (BbeIm), (DinI), (EgeI), (EheIm), (FastDigest® EheIm), (KasIm), Mly113I, (SfoIm) NciI CC^SGG BcnI, FastDigest® NciI (BcnI) AsuC2I, BcnI, FastDigest® NciI (BcnI), BpuMI NcoI,
FastDigest® NcoIC^CATGG NcoI, FastDigest® NcoI Bsp19I NdeI,
FastDigest® NdeICA^TATG NdeI, FastDigest® NdeI FauNDI NdeII ^GATC Bsp143Im, (DpnI, FastDigest® DpnI) (GA^TC), MboIm, FastDigest® MboIm BfuCIm, Bsp143Im, BssMI, (BstKTI), BstMBI, (DpnI), (FastDigest® DpnI), DpnII, Kzo9Im, (MalI), MboIm, FastDigest® MboIm, Sau3AIm NgoMIV G^CCGGC (PdiI) (GCC^GGC) MroNI, (NaeI), (PdiI) NheI G^CTAGC NheI AsuNHI, (BmtIm) NlaIII CATG^ Hin1II (CviAIIm), FaeI, (FatIm), Hin1II, Hsp92II NlaIV GGN^NCC BspLIm, FastDigest® NlaIV (BspLI)m BmiI, BspLIm, FastDigest® NlaIV (BspLI)m, PspN4I NmuCI,
FastDigest® NmuCI^GTSAC NmuCI, FastDigest® NmuCI Tsp45I NotI,
FastDigest® NotIGC^GGCCGC NotI, FastDigest® NotI CciNI NruI TCG^CGA Bsp68I Bsp68I, BtuMI NsbI,
FastDigest® FspI (NsbI)TGC^GCA NsbI, FastDigest® FspI (NsbI) Acc16I, AviII, FspI NsiI ATGCA^T FastDigest® NsiI (Mph1103I), Mph1103I EcoT22Im, FastDigest® NsiI (Mph1103I), Mph1103I, Zsp2I NspI RCATG^Y XceI BstNSI, XceI NspV TT^CGAA Bsp119I, FastDigest® Bsp119I AsuIIm, Bpu14I, Bsp119I, FastDigest® Bsp119I, BspT104I, BstBIm, Csp45Im, SfuIm OliI CACNN^NNGTG OliI AleI
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Updated sausio 31, 2008 09:22
