Alphabetic List of Commercially Available Restriction Enzymes (C--G)
A number of restriction enzymes discovered by Fermentas are isoschizomers of commonly used prototype restriction enzymes. The following table will help you find the appropriate Fermentas enzymes for your experiments.
A B C-D-E-F-G H-I-K-L-M-N-O P-R-S-T-V-X-Z
Note
- Enzymes in parentheses have different cleavage specificities.
- Isoschizomers with different sensitivity to methylation are indicated by "m".
- DpnI requires the presence of N6-methyladenine within the recognition sequence GATC.
- Enzymes produced by Fermentas appear in green.
Single letter code: R = G or A;
Y = C or T;
W = A or T;
M = A or C;K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;B = C, G or T;
D = A, G or T;
N = G, A, T or C.
Enzyme Specificity 5'=>3' Fermentas enzyme Commercially available isoschizomers Cac8I GCN^NGC BstC8I CaiI,
FastDigest® AlwNI (CaiI)CAGNNN^CTG CaiI, FastDigest® AlwNI (CaiI) AlwNI CciNI GC^GGCCGC NotI, FastDigest® NotI NotI, FastDigest® NotI CelII GC^TNAGC Bpu1102I, FastDigest® BlpI (Bpu1102I) BlpI, Bpu1102I, FastDigest® BlpI (Bpu1102I), Bsp1720I CfoI GCG^C HhaI, FastDigest® HhaI, (Hin6I, FastDigest® HinP1I (Hin6I)) (G^CGC) AspLEI, BstHHI, HhaI, FastDigest® HhaI, (Hin6I), (FastDigest® HinP1I (Hin6I)), (HinP1I), (HspAI) Cfr10I,
FastDigest® BsrFI (Cfr10I)R^CCGGY Cfr10I, FastDigest® BsrFI (Cfr10I) Bse118I, BsrFI, BssAIm Cfr13I,
FastDigest® Sau96I (Cfr13I)G^GNCC Cfr13I, FastDigest® Sau96I (Cfr13I) AspS9I, (BmgT120I), Sau96I Cfr42I CCGC^GG Cfr42I KspI, SacII, Sfr303I, SgrBI, SstII Cfr9I C^CCGGG Cfr9I, (SmaIm) (CCC^GGG),
(FastDigest® SmaIm) (CCC^GGG)(SmaIm), (FastDigest® SmaIm), TspMIm, XmaCI, XmaIm CfrI Y^GGCCR CfrI, CfrI EaeI CfrI Y^GGCCR CfrI, CfrI EaeI ClaI AT^CGAT FastDigest® ClaI (Bsu15I), Bsu15I BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, FastDigest® ClaI (Bsu15I), Bsu15I, BsuTUI CpoI CG^GWCCG CpoI CspI, Rsr2I, RsrII CseI GACGC(5/10)^ CseI HgaI Csp45I TT^CGAA Bsp119I, FastDigest® Bsp119I AsuII, Bpu14I, Bsp119I, FastDigest® Bsp119I, BspT104I, BstBI, NspVm, SfuI Csp6I,
FastDigest® Csp6IG^TAC Csp6I, FastDigest® Csp6I, (RsaIm) (GT^AC), (FastDigest® RsaIm)(GT^AC) (AfaIm), (RsaIm), (FastDigest® RsaIm) CspAI A^CCGGT BshTIm, FastDigest® AgeI (BshTI)m AgeIm, AsiGI, BshTIm, FastDigest® AgeI (BshTI)m, PinAI CspCI ^(11/13)CAA(N)5GTGG(12/10)^ CspI CG^GWCCG CpoI CpoI, Rsr2I, RsrII CviAII C^ATG (Hin1II) (CATG^) (FaeI), (FatIm), (Hin1II), (Hsp92II), (NlaIIIm) CviJI RG^CY CviKI-1 CviKI-1 RG^CY CviJI DdeI C^TNAG HpyF3I, FastDigest® DdeI (HpyF3I) BstDEI, HpyF3I, FastDigest® DdeI (HpyF3I) DinI GGC^GCC EheI, FastDigest® EheI (BbeI), EgeI, EheI, FastDigest® EheI, (KasI), (Mly113I), (NarI), SfoI DpnI,
FastDigest® DpnIGA^TC (Bsp143I) (^GATC), DpnI, FastDigest® DpnI, (MboI, FastDigest® MboI) (^GATC) (BfuCI), (Bsp143I), (BssMI), (BstKTI), (BstMBI), (DpnII), (Kzo9I), MalI, (MboI), (FastDigest® MboI), (NdeII), (Sau3AI) DpnII ^GATC Bsp143Im, (DpnI, FastDigest® DpnI) (GA^TC), MboIm, FastDigest® MboIm BfuCIm, Bsp143Im, BssMI, (BstKTI), BstMBI, (DpnI), (FastDigest® DpnI), Kzo9Im, (MalI), MboIm, FastDigest® MboIm, NdeII, Sau3AIm DraI,
FastDigest® DraITTT^AAA DraI, FastDigest® DraI DraII RG^GNCCY EcoO109I EcoO109I DraIII CACNNN^GTG AdeI, FastDigest® DraIII (AdeI) AdeI, FastDigest® DraIII (AdeI) DrdI GACNNNN^NNGTC AasI AasI, DseDI DriI GACNNN^NNGTC Eam1105I, FastDigest® Eam1105I AhdI, AspEI, BmeRI, Eam1105I, FastDigest® Eam1105I, EclHKI DseDI GACNNNN^NNGTC AasI AasI, DrdI EaeI Y^GGCCR CfrI, CfrI CfrI, CfrI EagI C^GGCCG Eco52I BseX3I, BstZI, EclXI, Eco52I Eam1104I CTCTTC(1/4)^ Eam1104I Bst6I, EarI, Ksp632I Eam1105I,
FastDigest® Eam1105IGACNNN^NNGTC Eam1105I, FastDigest® Eam1105I AhdI, AspEI, BmeRI, DriI, EclHKI EarI CTCTTC(1/4)^ Eam1104I Bst6I, Eam1104I, Ksp632I EciI GGCGGA(11/9)^ Ecl136II,
FastDigest® Ecl136IIGAG^CTC Ecl136IIm, FastDigest® Ecl136IIm, (SacIm) (GAGCT^C),
(FastDigest® SacIm) (GAGCT^C)EcoICRI, (Psp124BI), (SacIm), (FastDigest® SacIm), (SstIm) EclHKI GACNNN^NNGTC Eam1105I, FastDigest® Eam1105I AhdI, AspEI, BmeRI, DriI, Eam1105I, FastDigest® Eam1105I EclXI C^GGCCG Eco52I BseX3I, BstZI, EagI, Eco52I Eco105I,
FastDigest® SnaBI (Eco105I)TAC^GTA Eco105I, FastDigest® SnaBI (Eco105I) BstSNI, SnaBI Eco130I,
FastDigest® StyI (Eco130I)C^CWWGG Eco130I, FastDigest® StyI (Eco130I) BssT1I, EcoT14I, ErhI, StyI Eco147I,
FastDigest® StuI (Eco147I)AGG^CCT Eco147I, FastDigest® StuI (Eco147I) AatI, PceI, SseBI, StuI Eco24I GRGCY^C Eco24I BanII, EcoT38I, FriOI Eco31I,
FastDigest® Eco31IGGTCTC(1/5)^ Eco31I, FastDigest® Eco31I BsaI, Bso31I, BspTNI Eco32I,
FastDigest® EcoRV (Eco32I)GAT^ATC Eco32I, FastDigest® EcoRV (Eco32I) EcoRV Eco47I,
FastDigest® AvaII (Eco47I)G^GWCC Eco47I, FastDigest® AvaII (Eco47I) AvaII, Bme18I, SinI, VpaK11BI Eco47III,
FastDigest® AfeI (Eco47III)AGC^GCT Eco47III, FastDigest® AfeI (Eco47III) AfeI, Aor51HI Eco52I C^GGCCG Eco52I BseX3I, BstZI, EagI, EclXI Eco57I CTGAAG(16/14)^ Eco57I AcuI Eco57MI CTGRAG(16/14)^ Eco57MI Eco72I CAC^GTG Eco72I AcvI, BbrPI, PmaCI, PmlI, PspCI Eco81I CC^TNAGG Eco81I AxyI, Bse21I, Bsu36I Eco88I,
FastDigest® AvaI (Eco88I)C^YCGRG FastDigest® AvaI (Eco88I), Eco88I Ama87I, AvaIm, (BmeT110Im), BsiHKCI, BsoBIm Eco91I,
FastDigest® Eco91IG^GTNACC Eco91I, FastDigest® Eco91I BstEII, BstPI, EcoO65I, PspEI EcoICRI GAG^CTC Ecl136II, FastDigest® Ecl136II, (SacI) (GAGCT^C),
(FastDigest® SacI) (GAGCT^C)Ecl136II, FastDigest® Ecl136II, (Psp124BI), (SacI), (FastDigest® SacI), (SstI) EcoNI CCTNN^NNNAGG XagI, FastDigest® EcoNI (XagI) BstENI, XagI, FastDigest® EcoNI (XagI) EcoO109I RG^GNCCY EcoO109I DraII EcoO65I G^GTNACC Eco91I, FastDigest® Eco91I BstEII, BstPI, Eco91I, FastDigest® Eco91I, PspEI EcoP15I CAGCAG(25/27)^ EcoRI,
FastDigest® EcoRIG^AATTC EcoRI, FastDigest® EcoRI EcoRII ^CCWGG EcoRII, (MvaIm) (CC^WGG),
(FastDigest® MvaIm) (CC^WGG)AjnIm, (BptI), (BseBI), (Bst2UIm), (BstNIm), (BstOIm), (MvaIm), (FastDigest® MvaIm), Psp6I, PspGI EcoRV GAT^ATC Eco32I, FastDigest® EcoRV (Eco32I) Eco32I, FastDigest® EcoRV (Eco32I) EcoT14I C^CWWGG Eco130I, FastDigest® StyI (Eco130I) BssT1I, Eco130I, FastDigest® StyI (Eco130I), ErhI, StyI EcoT22I ATGCA^T FastDigest® NsiI (Mph1103I), Mph1103I FastDigest® NsiI (Mph1103I), Mph1103I, NsiIm, Zsp2I EcoT38I GRGCY^C Eco24I BanII, Eco24I, FriOI EgeI GGC^GCC EheI, FastDigest® EheI (BbeI), DinI, EheI, FastDigest® EheI, (KasI), (Mly113I), (NarI), SfoI EheI,
FastDigest® EheIGGC^GCC EheI, FastDigest® EheI (BbeI), DinI, EgeI, (KasIm), (Mly113I), (NarIm), SfoIm ErhI C^CWWGG Eco130I, FastDigest® StyI (Eco130I) BssT1I, Eco130I, FastDigest® StyI (Eco130I), EcoT14I, StyI Esp3I CGTCTC(1/5)^ Esp3I BsmBI FaeI CATG^ Hin1II (CviAII), (FatI), Hin1II, Hsp92II, NlaIII FalI ^(8/13)AAG(N)5CTT(13/8)^ FaqI GGGAC(10/14)^ FaqI BslFI, BsmFI FatI ^CATG (Hin1II)(CATG^) (CviAIIm), (FaeI), (Hin1II), (Hsp92II), (NlaIIIm) FauI CCCGC(4/6)^ SmuI SmuI FauNDI CA^TATG NdeI, FastDigest® NdeI NdeI, FastDigest® NdeI FbaI T^GATCA BclI, FastDigest® BclI BclI, FastDigest® BclI, Ksp22I FblI GT^MKAC XmiI, FastDigest® AccI (XmiI) AccI, XmiI, FastDigest® AccI (XmiI) Fnu4HI GC^NGC SatI, FastDigest® Fnu4HI (SatI) BisI, Fsp4HI, ItaIm, SatI, FastDigest® Fnu4HI (SatI) FokI GGATG(9/13)^ (BseGI) (GGATG(2/0)), (FastDigest® BseGI) (GGATG(2/0)) (BseGI), (FastDigest® BseGI), (BstF5I), (BtsCI) FriOI GRGCY^C Eco24I BanII, Eco24I, EcoT38I FseI GGCCGG^CC Fsp4HI GC^NGC SatI, FastDigest® Fnu4HI (SatI) BisI, Fnu4HI, ItaIm, SatI, FastDigest® Fnu4HI (SatI) FspAI,
FastDigest® FspAIRTGC^GCAY FspAI, FastDigest® FspAI FspBI,
FastDigest® BfaI (FspBI)C^TAG FspBI, FastDigest® BfaI (FspBI) BfaI, MaeI, XspI FspI TGC^GCA NsbI, FastDigest® FspI (NsbI) Acc16I, AviII, NsbI, FastDigest® FspI (NsbI) GsuI CTGGAG(16/14)^ GsuI BpmIm
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Updated sausio 31, 2008 09:22
