Alphabetic List of Commercially Available Restriction Enzymes (A)
A number of restriction enzymes discovered by Fermentas are isoschizomers of commonly used prototype restriction enzymes. The following table will help you find the appropriate Fermentas enzymes for your experiments.
A B C-D-E-F-G H-I-K-L-M-N-O P-R-S-T-V-X-Z
Note
- Enzymes in parentheses have different cleavage specificities.
- Isoschizomers with different sensitivity to methylation are indicated by "m".
- DpnI requires the presence of N6-methyladenine within the recognition sequence GATC.
- Enzymes produced by Fermentas appear in green.
Single letter code: R = G or A;
Y = C or T;
W = A or T;
M = A or C;K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;B = C, G or T;
D = A, G or T;
N = G, A, T or C.
Enzyme Specificity 5'=>3' Fermentas enzyme Commercially available isoschizomers AarI CACCTGC(4/8)^ AarI AasI GACNNNN^NNGTC AasI DrdI, DseDI AatI AGG^CCT Eco147I, FastDigest® StuI (Eco147I) Eco147I, FastDigest® StuI (Eco147I), PceI, SseBIm, StuI AatII GACGT^C AatII (ZraIm) Acc16I TGC^GCA NsbI, FastDigest® FspI (NsbI) AviII, FspI, NsbI, FastDigest® FspI (NsbI) Acc36I ACCTGC(4/8)^ BveI BfuAI, BspMI, BveI Acc65I,
FastDigest® Acc65IG^GTACC Acc65I, FastDigest® Acc65I, (KpnIm) (GGTAC^C), (FastDigest® KpnIm) (GGTAC^C) Asp718Im, (KpnIm), (FastDigest® KpnIm) AccB1I G^GYRCC BshNI, FastDigest® BanI (BshNI) BanI, BshNI, FastDigest® BanI (BshNI), BspT107I AccB7I CCANNNN^NTGG Van91I, FastDigest® PflMI (Van91I) PflMI, Van91I, FastDigest® PflMI (Van91I) AccBSI CCGCTC(-3/-3)^ MbiI BsrBI, MbiI AccI GT^MKAC XmiI, FastDigest® AccI (XmiI) FblI, XmiI, FastDigest® AccI (XmiI) AccII CG^CG Bsh1236I, FastDigest® Bsh1236I Bsh1236I, FastDigest® Bsh1236I, BstFNI, BstUI, MvnI AccIII T^CCGGA Kpn2Im, FastDigest® Kpn2Im Aor13HI, BlfI, BseAIm, Bsp13I, BspEIm, Kpn2Im, FastDigest® Kpn2Im, MroIm AciI CCGC(-3/-1)^ SsiI, FastDigest® AciI (SsiI) BspACI, SsiI, FastDigest® AciI (SsiI) AclI AA^CGTT Psp1406I, FastDigest® AclI (Psp1406I) Psp1406I, FastDigest® AclI (Psp1406) AclWI GGATC(4/5)^ BspPI AlwI, BspPI AcoI Y^CCGGR AcsI R^AATTY XapI, FastDigest® XapI ApoI, XapI, FastDigest® XapI AcuI CTGAAG(16/14)^ Eco57I Eco57I AcvI CAC^GTG Eco72I BbrPI, Eco72I, PmaCI, PmlI, PspCI AcyI GR^CGYC Hin1I, FastDigest® BsaHI (Hin1I) BsaHI, BssNI, BstACI, Hin1I, FastDigest® BsaHI (Hin1I), Hsp92I AdeI,
FastDigest® DraIII (AdeI)CACNNN^GTG AdeI, FastDigest® DraIII (AdeI) DraIII AfaI GT^AC (Csp6Im, FastDigest® Csp6Im) (G^TAC), RsaI, FastDigest® RsaI (Csp6Im), (FastDigest® Csp6Im), RsaI, FastDigest® RsaI AfeI AGC^GCT Eco47III, FastDigest® AfeI (Eco47III) Aor51HI, Eco47III, FastDigest® AfeI (Eco47II) AfiI CCNNNNN^NNGG BseLI, FastDigest® BslI (BseLI) Bsc4I, BseLI, FastDigest® BslI (BseLI), BsiYI, BslI AflII C^TTAAG BspTI BfrI, BspTI, Bst98I, MspCI, Vha464I AflIII A^CRYGT AgeI A^CCGGT BshTI, FastDigest® AgeI (BshTI) AsiGI, BshTI, FastDigest® AgeI (BshTI), CspAIm, PinAI AhdI GACNNN^NNGTC Eam1105I, FastDigest® Eam1105I AspEI, BmeRI, DriI, Eam1105I, FastDigest® Eam1105I, EclHKI AhlI A^CTAGT BcuI, FastDigest® SpeI (BcuI) BcuI, FastDigest® SpeI (BcuI), SpeI AjiI CACGTC(-3/-3)^ AjiI BmgBI, BtrI AjnI ^CCWGG EcoRIIm, (MvaI) (CC^WGG),
(FastDigest® MvaI)(CC^WGG)(BptI), (BseBI), (Bst2UI), (BstNI), (BstOI), EcoRIIm, (MvaI), (FastDigest® MvaI), Psp6I, PspGIm AjuI ^(7/12)GAA(N)7TTGG(11/6)^ AjuI AleI CACNN^NNGTG OliI OliI AlfI ^(10/12)GCA(N)6TGC(12/10)^ AlfI AloI ^(7/12)GAAC(N)6TCC(12/7)^ AloI AluI AG^CT AluI Alw21I,
FastDigest® Alw21IGWGCW^C Alw21I, FastDigest® Alw21I Bbv12I, BsiHKAI Alw26I,
FastDigest® Alw26IGTCTC(1/5)^ Alw26I, FastDigest® Alw26I BsmAIm, BstMAI Alw44I G^TGCAC Alw44I ApaLIm, VneI AlwI GGATC(4/5)^ BspPI AclWI, BspPI AlwNI CAGNNN^CTG CaiI, FastDigest® AlwNI (CaiI) CaiI, FastDigest® AlwNI (CaiI) Ama87I C^YCGRG FastDigest® AvaI (Eco88I), Eco88I AvaI, (BmeT110I), BsiHKCI, BsoBI, FastDigest® AvaI (Eco88I), Eco88I Aor13HI T^CCGGA Kpn2I, FastDigest® Kpn2I AccIII, BlfI, BseAI, Bsp13I, BspEI, Kpn2I, FastDigest® Kpn2I, MroI Aor51HI AGC^GCT Eco47III, FastDigest® AfeI (Eco47III) AfeI, Eco47III, FastDigest® AfeI (Eco47III) ApaI,
FastDigest® ApaIGGGCC^C ApaI, FastDigest® ApaI, (Bsp120I, FastDigest® Bsp120I) (G^GGCCC) (Bsp120I), (FastDigest® Bsp120I), (PspOMI) ApaLI G^TGCAC Alw44Im Alw44Im, VneI ApeKI G^CWGC TseI ApoI R^AATTY XapI, FastDigest® XapI AcsI, XapI, FastDigest® XapI AscI GG^CGCGCC SgsI, FastDigest® AscI (SgsI) PalAI, SgsI, FastDigest® AscI (SgsI) AseI AT^TAAT VspI, FastDigest® AseI (VspI) PshBI, VspI, FastDigest® AseI (VspI) AsiGI A^CCGGT BshTI, FastDigest® AgeI (BshTI) AgeI, BshTI, FastDigest® AgeI (BshTI), CspAI, PinAI AsiSI GCGAT^CGC RgaI, SgfI Asp700I GAANN^NNTTC PdmI, FastDigest® PdmI MroXI, PdmI, FastDigest® PdmI, XmnI Asp718I G^GTACC Acc65Im, FastDigest® Acc65Im, (KpnIm) (GGTAC^C),
(FastDigest® KpnIm) (GGTAC^C)Acc65Im, FastDigest® Acc65Im, (KpnIm), (FastDigest® KpnIm) AspA2I C^CTAGG XmaJI, FastDigest® AvrII (XmaJI) AvrII, BlnI, XmaJI, FastDigest® AvrII (XmaJI) AspEI GACNNN^NNGTC Eam1105I, FastDigest® Eam1105I AhdI, BmeRI, DriI, Eam1105I, FastDigest® Eam1105I, EclHKI AspI GACN^NNGTC PsyI PflFI, PsyI, Tth111I AspLEI GCG^C HhaI, FastDigest® HhaI, (Hin6I, FastDigest® HinP1I (Hin6I)) (G^CGC) BstHHI, CfoI, HhaI, FastDigest® HhaI, (Hin6I), (FastDigest® HinP1I (Hin6I)), (HinP1I), (HspAI) AspS9I G^GNCC Cfr13I, FastDigest® Sau96I (Cfr13I) (BmgT120I), Cfr13I, FastDigest® Sau96I (Cfr13I), Sau96I AssI AGT^ACT ScaI BmcAI, ScaI, ZrmI AsuC2I CC^SGG BcnI, FastDigest® NciI (BcnI) BcnI, FastDigest® NciI (BcnI), BpuMI, NciI AsuHPI GGTGA(8/7)^ HphI HphI AsuII TT^CGAA Bsp119Im, FastDigest® Bsp119Im Bpu14I, Bsp119Im, FastDigest® Bsp119Im, BspT104Im, BstBIm, Csp45I, NspVm, SfuI AsuNHI G^CTAGC NheI (BmtI), NheI AvaI C^YCGRG FastDigest® AvaI (Eco88I)m, Eco88Im Ama87I, (BmeT110Im), BsiHKCI, BsoBIm, FastDigest® AvaI (Eco88I)m, Eco88Im AvaII G^GWCC Eco47I, FastDigest® AvaII (Eco47I) Bme18I, Eco47I, FastDigest® AvaII (Eco47I), SinI, VpaK11BI AviII TGC^GCA NsbI, FastDigest® FspI (NsbI) Acc16I, FspI, NsbI, FastDigest® FspI (NsbI) AvrII C^CTAGG XmaJI, FastDigest® AvrII (XmaJI) AspA2I, BlnI, XmaJI, FastDigest® AvrII (XmaJI) AxyI CC^TNAGG Eco81I Bse21I, Bsu36I, Eco81I
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Updated sausio 31, 2008 09:22
