Support, restriction enzymes: Chart "Reaction Conditions for Restriction Enzymes"

Restriction Enzymes

Modifying Enzymes

PCR, qPCR, RT-PCR & dNTPs

Molecular Cloning

Nucleic Acid Purification

Molecular Labeling & Detection

In vitro Transcription

Electrophoresis Products

Nucleotides

Transfection Reagents

Reagents

Reaction Conditions for Restriction Enzymes:

General Protocol for DNA Digestion

Five Buffer System

Dilution of Restriction Enzymes

Stability During Prolonged Incubation

Inactivation

Considerations for Partial Digestion of DNA

Chart: Reaction Conditions for Restriction Enzymes (see below)

Chart: Reaction Conditions for Restriction Enzymes

Note
Reactions were carried out at 37°C except for AloI, BclI, BdaI, BseDI, BseGI, BseJI, BseLI, BseMI, BseMII, BseNI, BseSI, BseXI, BspPI, BstXI, GsuI, Kpn2I, PpiI, Ppu21I, SmaI, SmiI, SmoI, TaaI, TaiI, TasI, TaqI, TauI, TatI, Tru1I and TsoI. The reaction temperature for these enzymes is indicated in parenthesis.
80°C(10 u) indicates that only small amounts of enzyme (up to 10 units) can be inactivated at 80°C in 20 minutes.
If restriction enzyme is thermostable, phenol deproteinization and/or ethanol precipitation of the fragments after the restriction reaction should be performed.
* Star activity appears at 5-fold overdigestion (5 units x 1 hour).
NR: Buffer is not recommended, because of high star activity.

For double digestion recommendations, see.

A B CD EF GHKL MNO PR STVX

A

Enzyme Recom-
mended
buffer Units for
overnight
incubation,
u/µg DNA Thermal
inacti-
vation,
in 20 min
Enzyme activity, %

B (blue)
1X G (green)
1X O (orange)
1X R (red)
1X Tango™ (yellow)

1X 2X

AanI Tango 0.5 65°C 50-100 50-100 0-20 0-20 100 20-50

AarI AarI
+oligo 1.0 65°C NR NR 0-20
(+oligo) 0-20
(+oligo) NR 50-100
(+oligo)

AasI B 0.1 80°C 100 20-50 0-20 0-20 50-100* 0-20

AatII Tango 0.3 65°C 50-100 20-50 0-20 0-20 100 20-50

Acc65I O 0.3 65°C 0-20 20-50 100 20-50 20-50 50-100

AdeI G 0.5 No 0-20 100 20-50 100 100* 20-50

AjiI AjiI 0.5 65°C NR NR 20-50* NR NR 20-50*

AjuI R
+SAM 0.5 65°C 0-20
(+SAM) 50-100
(+SAM) 20-50
(+SAM) 100
(+SAM) 50-100
(+SAM) 50-100
(+SAM)

AlfI R
+SAM 1.0 65°C 0-20
(+SAM) 0-20
(+SAM) 0-20
(+SAM) 100
(+SAM) 0-20
(+SAM) 20-50
(+SAM)

AloI (30°C) R 0.1 65°C 0-20 0-20 0-20 100 20-50 100

AluI Tango 0.1 65°C 50-100 0-20 0-20 0-20 100 20-50

Alw21I O 0.1 65°C 0-20 20-50 100 50-100 20-50 50-100

Alw26I Tango 0.2 65°C 50-100 100 0-20 0-20 100 100

Alw44I Tango 0.1 65°C 50-100 100 0-20 50-100 100 50-100

ApaI B 0.2 65°C 100 20-50 0-20 0-20 20-50 0-20

B

Enzyme Recom-
mended
buffer Units for
overnight
incubation,
u/µg DNA Thermal
inacti-
vation,
in 20 min
Enzyme activity, %

B (blue)
1X G (green)
1X O (orange)
1X R (red)
1X Tango™ (yellow)

1X 2X

BamHI BamHI 0.5 80°C(10 u) 20-50* 100 20-50 50-100* 100* 50-100

BauI Tango 0.2 65°C 0-20 50-100 0-20 50-100 100 50-100

BclI (55°C) G 0.1 80°C(10 u) 20-50 100 20-50 20-50 100* 100

BcnI Tango 0.2 65°C 20-50 50-100 50-100 50-100 100 50-100

BcuI Tango 0.5 No 50-100 50-100 0-20 20-50 100 0-20

BdaI (30°C) G
+SAM 1.0 65°C NR 100
(+SAM) 0-20
(+SAM) 20-50
(+SAM) 50-100*
(+SAM) 50-100
(+SAM)

BfiI Tango 0.1 65°C 20-50 20-50 0-20 0-20 100 0-20

BfmI Tango 1.0 65°C 0-20 50-100 0-20 0-20 100 20-50

BfuI BfuI 1.0 80°C NR NR 0-20 0-20 NR 50-100*

BglI O 0.1 65°C 0-20 50-100 100 100 0-20 100

BglII O 0.1 No 0-20 20-50 100 50-100 0-20 100

Bme1390I O 0.2 80°C 20-50 50-100 100 50-100 50-100 50-100

BoxI Tango 0.5 80°C 0-20 0-20 0-20 20-50 100 20-50

BpiI G 0.3 65°C 20-50 100 50-100 50-100 50-100 50-100

BplI Tango
+SAM 0.3 65°C 0-20
(+SAM) 20-50
(+SAM) 0-20
(+SAM) 0-20
(+SAM) 100
(+SAM) 20-50
(+SAM)

Bpu10I Bpu10I 0.2 80°C 0-20 20-50* 50-100* 100* 50-100* 100*

Bpu1102I Tango 0.1 80°C 50-100 50-100 20-50 20-50 100 20-50

BseDI (55°C) Tango 0.2 80°C 50-100 20-50 0-20 0-20 100 50-100

BseGI (55°C) Tango 0.2 80°C 20-50 50-100 20-50 20-50 100 20-50

BseJI (65°C) O 0.1 No NR 100* 100 NR NR 100*

BseLI (55°C) Tango 0.1 No 20-50 100 50-100 20-50 100 50-100

BseMI (55°C) R 0.3 80°C 0-20 20-50 0-20 100 50-100 50-100

BseMII (55°C) Tango
+SAM 0.5 80°C 50-100
(+SAM) 50-100
(+SAM) 50-100
(+SAM) 50-100
(+SAM) 100
(+SAM) 50-100
(+SAM)

BseNI (65°C) B 0.1 80°C 100 20-50 0-20 0-20 50-100 20-50

BseSI (55°C) G 0.1 80°C(10 u) 20-50 100 0-20 20-50 50-100 0-20

BseXI (65°C) BseXI 0.3 80°C NR NR NR NR NR NR

Bsh1236I R 0.1 65°C 0-20 0-20 50-100 100 20-50 50-100

Bsh1285I G 0.2 65°C 20-50 100 20-50 0-20 0-20 20-50

BshNI O 0.3 65°C 0-20 20-50 100 50-100 0-20 100

BshTI O 0.1 65°C 0-20 20-50 100 50-100 20-50 20-50

Bsp68I O 0.2 65°C 0-20 20-50 100 50-100 20-50 50-100

Bsp119I Tango 0.1 80°C 20-50 0-20 0-20 0-20 100 100

Bsp120I B 0.1 80°C 100 20-50 0-20 20-50 50-100 0-20

Bsp143I Bsp143I 0.1 65°C 20-50 20-50 0-20 0-20 50-100 20-50

Bsp1407I Tango 0.1 65°C 0-20 20-50 0-20 20-50 100 50-100

BspLI Tango 0.3 65°C 50-100 50-100 0-20 20-50 100 20-50

BspOI O 0.2 80°C 0-20 0-20 100 100 0-20 20-50

BspPI (55°C) Tango 0.5 80°C 20-50 20-50 0-20 0-20 100 0-20

BspTI O 0.1 65°C 0-20 0-20 100 20-50 0-20 50-100

Bst1107I O 0.1 65°C 20-50 50-100 100 100 20-50 100

BstXI (55°C) O 0.1 65°C 20-50 100 100 50-100 50-100 100

Bsu15I Tango 0.1 65°C 20-50 20-50 20-50 20-50 100 20-50

BsuRI R 0.1 80°C 20-50 20-50 50-100 100 50-100 100

BveI O
+oligo 0.2 65°C 0-20
(+oligo) 20-50
(+oligo) 100
(+oligo) 20-50
(+oligo) 50-100
(+oligo) 100
(+oligo)

CD

Enzyme Recom-
mended
buffer Units for
overnight
incubation,
u/µg DNA Thermal
inacti-
vation,
in 20 min
Enzyme activity, %

B (blue)
1X G (green)
1X O (orange)
1X R (red)
1X Tango™ (yellow)

1X 2X

CaiI Tango 0.2 65°C 20-50 20-50 20-50 50-100 100 50-100

CfrI Tango 1.0 65°C 50-100* 50-100 0-20 0-20 100 0-20

Cfr9I Cfr9I 0.2 65°C 0-20 0-20 0-20 0-20 20-50 0-20

Cfr10I Cfr10I 0.1 No 0-20 20-50 20-50 50-100 20-50 50-100

Cfr13I Tango 0.3 65°C 50-100 50-100 20-50 20-50 100 20-50

Cfr42I B 0.1 65°C 100 50-100 0-20 0-20 50-100 0-20

CpoI Tango 0.5 65°C 20-50 50-100 50-100 20-50 100 50-100

CseI R 0.5 80°C(10 u) NR 50-100* 50-100 100 100* 50-100

Csp6I B 0.1 65°C 100 50-100 0-20 0-20 50-100 0-20

DpnI Tango 0.1 80°C 100 100 50-100 50-100 100 50-100

DraI Tango 0.1 65°C 50-100 50-100 20-50 20-50 100 50-100

EF

Enzyme Recom-
mended
buffer Units for
overnight
incubation,
u/µg DNA Thermal
inacti-
vation,
in 20 min
Enzyme activity, %

B (blue)
1X G (green)
1X O (orange)
1X R (red)
1X Tango™ (yellow)

1X 2X

Eam1104I Tango 0.5 65°C 50-100 50-100 0-20 0-20 100 0-20

Eam1105I Eam1105I 0.1 65°C 20-50 50-100 0-20 0-20 50-100 20-50

Ecl136II Ecl136II 0.2 65°C 50-100 20-50 0-20 0-20 50-100 0-20

Eco24I Tango 0.2 65°C 50-100 50-100 0-20 20-50 100 0-20

Eco31I G 0.3 65°C 50-100 100 0-20 0-20 50-100 20-50

Eco32I R 0.1 65°C 0-20 50-100 50-100 100 20-50 100

Eco47I R 0.3 65°C 0-20 50-100 50-100 100 50-100 50-100

Eco47III O 0.1 65°C 0-20 20-50 100 100 50-100 100

Eco52I Eco52I 0.2 65°C 0-20 0-20 0-20 20-50 0-20 20-50

Eco57I G
+SAM 1.0 65°C 100
(+SAM) 100
( +SAM) 20-50
( +SAM) 20-50
( +SAM) 50-100
( +SAM) 50-100
( +SAM)

Eco57MI B
+SAM 1.0 65°C 100
( +SAM) 50-100
( +SAM) 0-20
( +SAM) 20-50
( +SAM) 50-100
( +SAM) 0-20
( +SAM)

Eco72I Tango 0.5 65°C NR NR 0-20 0-20 100 20-50

Eco81I Tango 0.1 80°C 50-100 100 0-20 0-20 100 0-20

Eco88I Tango 0.2 65°C 100 50-100 0-20 0-20 100 20-50

Eco91I O 0.1 65°C 20-50 20-50 100 50-100 50-100 100

Eco105I Tango 0.5 65°C 100* 50-100 0-20 0-20 100 0-20

Eco130I O 0.2 65°C 0-20 20-50 100 50-100 50-100 100

Eco147I B 0.1 80°C 100 50-100 20-50 20-50 50-100 0-20

EcoO109I Tango 0.2 65°C 50-100 20-50 20-50 20-50 100 100

EcoRI EcoRI 0.2 65°C 0-20 NR 100 100* NR 100

EcoRII O 0.1 80°C 20-50 50-100 100 50-100 20-50 50-100

EheI Tango 1.0 65°C 20-50 50-100 0-20 0-20 100 20-50

Esp3I Tango
+DTT 0.2 65°C 100
(+DTT) 20-50
(+DTT) 0-20
(+DTT) 0-20
(+DTT) 100
(+DTT) 0-20
(+DTT)

FaqI Tango
+SAM 1.0 80°C 20-50
( +SAM) 20-50
( +SAM) 0-20
( +SAM) 0-20
( +SAM) 100
( +SAM) 20-50
( +SAM)

FspAI O 0.2 65°C 0-20 0-20 100 50-100 0-20 50-100

FspBI Tango 0.3 65°C 50-100 20-50 0-20 0-20 100 0-20

GHKL

Enzyme Recom-
mended
buffer Units for
overnight
incubation,
u/µg DNA Thermal
inacti-
vation,
in 20 min
Enzyme activity, %

B (blue)
1X G (green)
1X O (orange)
1X R (red)
1X Tango™ (yellow)

1X 2X

GsuI (30°C) B 1.0 65°C 100 50-100 20-50 20-50 100 50-100

HhaI Tango 0.1 80°C 50-100 50-100 20-50 20-50 100 20-50

Hin1I G 0.1 65°C 20-50 100 20-50 20-50 20-50 20-50

Hin1II G 0.3 65°C 50-100 100 20-50 50-100 50-100 50-100

Hin4I Tango
+SAM 0.2 65°C 20-50
( +SAM) 20-50
( +SAM) 0-20
( +SAM) 0-20
( +SAM) 100
( +SAM) 0-20
( +SAM)

Hin6I Tango 0.1 65°C 50-100 50-100 20-50 20-50 100 50-100

HincII Tango 0.1 65°C 50-100 50-100 20-50 50-100 100 50-100

HindIII R 0.1 65°C 0-20 20-50 0-20 100 50-100 50-100

HinfI R 0.1 65°C 0-20 20-50 50-100 100 50-100 50-100

HpaII Tango 0.1 65°C 50-100 50-100 0-20 20-50 100 20-50

HphI B 0.1 65°C 100 0-20 0-20 0-20 20-50 0-20

Hpy8I Tango 0.1 80°C 50-100 50-100 0-20 20-50 100 50-100

HpyF3I Tango 0.2 65°C 20-50 20-50 20-50 20-50 100 50-100

HpyF10VI Tango 0.1 80°C 0-20 0-20 0-20 0-20 100 50-100

KpnI KpnI 0.2 80°C 20-50 0-20 0-20 0-20 20-50 0-20

Kpn2I (55°C) Tango 0.1 80°C 50-100 50-100 0-20 20-50 100 50-100

KspAI B 0.5 65°C 100 50-100* 20-50 20-50 100* 50-100

LguI Tango 0.5 65°C 20-50 50-100 20-50 20-50 100 20-50

Lsp1109I Lsp1109I 0.5 65°C 0-20 20-50* 50-100* 100 20-50* 20-50*

LweI Tango 0.2 65°C 0-20 0-20 0-20 20-50 100 20-50

MNO

Enzyme Recom-
mended
buffer Units for
overnight
incubation,
u/µg DNA Thermal
inacti-
vation,
in 20 min
Enzyme activity, %

B (blue)
1X G (green)
1X O (orange)
1X R (red)
1X Tango™ (yellow)

1X 2X

MauBI Tango 0.1 65°C 0-20 0-20 0-20 0-20 100 0-20

MbiI Tango 0.2 65°C 20-50 100 20-50 20-50 100 20-50

MboI R 0.1 65°C 50-100 50-100 50-100 100 50-100 100

MboII B 1.0 65°C 100 50-100 20-50 0-20 50-100 20-50

MlsI R 0.5 65°C 0-20 20-50 0-20 100 20-50 50-100

MluI R 0.1 80°C 0-20 20-50 50-100 100 20-50 50-100

MnlI G 0.5 65°C 50-100 100 20-50 20-50 20-50 20-50

Mph1103I R 0.3 65°C 0-20 50-100 20-50 100 50-100 50-100

MreI G 0.5 80°C 20-50 100 0-20 0-20 50-100 0-20

MspI Tango 0.3 65°C 50-100 50-100 0-20 0-20 100 50-100

MssI B 0.5 65°C 100 0-20 0-20 0-20 20-50 0-20

MunI G 0.1 65°C 100 100 0-20 0-20 100 0-20

MvaI R 0.1 80°C(10 u) 20-50 20-50 50-100 100 20-50* 100

Mva1269I R 0.1 65°C 0-20 20-50 50-100 100 0-20 50-100

NcoI Tango 0.1 80°C 20-50 20-50 20-50 50-100 100 100

NdeI O 0.3 65°C 0-20 0-20 100 50-100 0-20 50-100

NheI Tango 0.2 65°C 100 20-50 0-20 0-20 100 0-20

NmuCI R 0.1 65°C 0-20 20-50 50-100 100 20-50 50-100

NotI O 0.1 65°C 0-20 0-20 100 20-50 0-20 20-50

NsbI Tango 0.1 65°C 20-50 50-100 0-20 20-50 100 20-50

OliI R 0.2 65°C 0-20 0-20 0-20 100 0-20 50-100

Enzyme Recom-
mended
buffer Units for
overnight
incubation,
u/µg DNA Thermal
inacti-
vation,
in 20 min
Enzyme activity, %

B (blue)
1X G (green)
1X O (orange)
1X R (red)
1X Tango™ (yellow)

1X 2X

PaeI B 0.1 65°C 100 50-100 0-20 0-20 50-100 0-20

PagI O 0.2 80°C 0-20 50-100 100 NR NR NR

PasI (55°C) PasI 0.2 80°C NR NR NR NR NR NR

PauI R 0.2 80°C 0-20 0-20 100 100 0-20 100

PscI Tango 0.2 65°C 20-50 20-50 0-20 0-20 100 0-20

PdiI Tango 0.5 65°C 50-100 20-50 0-20 0-20 100 50-100

PdmI Tango 0.5 65°C 20-50 50-100 0-20 0-20 100 0-20

PfeI O 0.1 65°C 0-20 20-50 100 50-100 20-50 50-100

Pfl23II Tango 0.3 65°C 20-50 50-100 20-50 20-50 100 0-20

PfoI Tango 0.1 65°C 0-20 20-50 50-100 0-20 100 50-100

PpiI (30°C) R 0.1 65°C 0-20 0-20 0-20 100 50-100 50-100

Ppu21I (30°C) Ppu21I 0.5 65°C 50-100* 100* 20-50 NR NR NR

Psp5II G 0.2 80°C 0-20 100 20-50 20-50 50-100 100

Psp1406I Tango 0.5 65°C 100 50-100 0-20 20-50 100 0-20

PstI O 0.2 80°C(10 u) 50-100 50-100 100 100 50-100 50-100

PsuI B 0.5 80°C 100 20-50 0-20 0-20 50-100 0-20

PsyI B 0.1 80°C 100 50-100 0-20 0-20 50-100 0-20

PvuI R 0.2 80°C(10 u) 0-20 20-50 50-100 100 50-100 100

PvuII G 0.2 80°C 50-100* 100 20-50 50-100 20-50* 20-50*

RsaI Tango 0.2 65°C 50-100 20-50 0-20 0-20 100 0-20

RseI R 0.5 65°C 0-20 50-100 50-100 100 20-50 100

STVX

Enzyme Recom-
mended
buffer Units for
overnight
incubation,
u/µg DNA Thermal
inacti-
vation,
in 20 min
Enzyme activity, %

B (blue)
1X G (green)
1X O (orange)
1X R (red)
1X Tango™ (yellow)

1X 2X

SacI SacI 0.2 65°C 50-100 20-50 0-20 0-20 50-100 20-50

SalI O 0.1 65°C 0-20 0-20 100 20-50 0-20 50-100

SatI G 0.1 65°C 20-50 100 20-50 20-50 50-100 20-50

ScaI ScaI 0.5 80°C(10 u) 0-20 0-20 0-20 0-20 0-20 0-20

SchI Tango 0.2 65°C 20-50 50-100 0-20 0-20 100 0-20

SdaI SdaI 0.3 65°C NR NR 0-20 0-20 NR 20-50

SduI SduI 0.3 65°C NR 50-100* 50-100 0-20 NR NR

SfaAI Tango 0.2 80°C 50-100 0-20 0-20 0-20 100 0-20

SfiI (50°C) G 0.2 80°C 50-100 100 20-50 0-20 100 0-20

SgrDI R 0.3 65°C 0-20 0-20 0-20 100 NR 100

SgsI Tango 0.1 65°C 0-20 0-20 0-20 50-100 100 50-100

SmaI (30°C) Tango 0.2 65°C 50-100 0-20 0-20 0-20 100 0-20

SmiI (30°C) O 0.1 65°C 0-20 0-20 100 20-50 0-20 20-50

SmoI (55°C) Tango 0.2 80°C 50-100 20-50 0-20 20-50 100 20-50

SmuI Tango 0.2 65°C 50-100 50-100 0-20 20-50 100 20-50

SsiI O 0.5 65°C NR 20-50 100 50-100 NR 100

SspI G 0.1 65°C 20-50 100 0-20 50-100 100 20-50

TaaI (65°C) Tango 0.2 No 0-20 0-20 0-20 50-100 100 100

TaiI (65°C) R 0.3 No 50-100 50-100 20-50 100 100 50-100

TaqI (65°C) TaqI 0.3 No 0-20 20-50 20-50 20-50 20-50 20-50

TasI (65°C) B 0.3 No 100 50-100 20-50 0-20 20-50 0-20

TatI (65°C) Tango 0.2 No NR 50-100* 20-50 20-50 100* 0-20

TauI (55°C) B 1.0 No 100 50-100 0-20 0-20 20-50 0-20

Tru1I (65°C) R 0.2 No 50-100 50-100 20-50 100 50-100 100

TscAI (65°C) Tango 0.2 No 50-100 50-100 20-50 20-50 100 20-50

TsoI (55°C) G
+SAM 1.0 80°C NR 100
(+SAM) 50-100
(+SAM) 0-20
(+SAM) 50-100
(+SAM) 20-50
(+SAM)

TstI R 0.1 No 0-20 0-20 0-20 100 0-20 100

Van91I R 0.1 65°C 0-20 50-100 50-100 100 20-50 50-100

VspI O 0.1 65°C 0-20 50-100 100 20-50 100 100

XagI R 0.1 65°C 0-20 20-50 50-100 100 20-50 50-100

XapI Tango 0.1 80°C 50-100 100 0-20 0-20 100 20-50

XbaI Tango 0.1 65°C 50-100 50-100 20-50 0-20 100 50-100

XceI Tango 0.2 65°C 50-100 0-20 0-20 0-20 100 0-20

XhoI R 0.1 80°C 0-20 50-100 50-100 100 20-50 100

XmaJI Tango 0.2 80°C 20-50 50-100 50-100 50-100 100 50-100

XmiI B 0.1 65°C 100 0-20 0-20 0-20 50-100 20-50

Nb.Bpu10I R 0.3 80°C 0-20 20-50 20-50 100 20-50 50-100

Nb.Mva1269I O 0.1 80°C 0-20 20-50 100 100 20-50 50-100

Nt.Bpu10I R 0.2 65°C 0-20 0-20 100 100 0-20 50-100

I-SceI Tango 0.5 65°C 50-100 50-100 50-100 50-100 100 50-100

catalog@fermentas.com

Updated sausio 24, 2008 16:02

Enzyme	Recom- mended buffer	Units for overnight incubation, u/µg DNA	Thermal inacti- vation, in 20 min	Enzyme activity, %
				B (blue) 1X	G (green) 1X	O (orange) 1X	R (red) 1X	Tango™ (yellow)
				B (blue) 1X	G (green) 1X	O (orange) 1X	R (red) 1X	1X	2X
AanI	Tango	0.5	65°C	50-100	50-100	0-20	0-20	100	20-50
AarI	AarI +oligo	1.0	65°C	NR	NR	0-20 (+oligo)	0-20 (+oligo)	NR	50-100 (+oligo)
AasI	B	0.1	80°C	100	20-50	0-20	0-20	50-100*	0-20
AatII	Tango	0.3	65°C	50-100	20-50	0-20	0-20	100	20-50
Acc65I	O	0.3	65°C	0-20	20-50	100	20-50	20-50	50-100
AdeI	G	0.5	No	0-20	100	20-50	100	100*	20-50
AjiI	AjiI	0.5	65°C	NR	NR	20-50*	NR	NR	20-50*
AjuI	R +SAM	0.5	65°C	0-20 (+SAM)	50-100 (+SAM)	20-50 (+SAM)	100 (+SAM)	50-100 (+SAM)	50-100 (+SAM)
AlfI	R +SAM	1.0	65°C	0-20 (+SAM)	0-20 (+SAM)	0-20 (+SAM)	100 (+SAM)	0-20 (+SAM)	20-50 (+SAM)
AloI (30°C)	R	0.1	65°C	0-20	0-20	0-20	100	20-50	100
AluI	Tango	0.1	65°C	50-100	0-20	0-20	0-20	100	20-50
Alw21I	O	0.1	65°C	0-20	20-50	100	50-100	20-50	50-100
Alw26I	Tango	0.2	65°C	50-100	100	0-20	0-20	100	100
Alw44I	Tango	0.1	65°C	50-100	100	0-20	50-100	100	50-100
ApaI	B	0.2	65°C	100	20-50	0-20	0-20	20-50	0-20