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Newly Generated Recognition Sequences Resulting from:

• Ligation of Blunt DNA Ends

• Ligation of Protruding Compatible DNA Ends
• Fill-in of a 5'-overhang and Self-ligation
• Removal of a 3'-overhang and Self-ligation (see below)

Newly Generated Recognition Sequences Resulting by Removal of a 3'-overhang and Self-ligation

Note

• [] denotes the enzymes that cleave the target both before and after the ligation.
• Enzymes produced by Fermentas are shown in green.

Single letter code:
R = G or A;  Y = C or T;  W = A or T;  M = A or C;	K = G or T;  S = C or G; H = A, C or T; V = A, C or G;	B = C, G or T; D = A, G or T; N = G, A, T or C.

Restriction enzyme	Recognition sequence	Newly generated sequence after reaction	Restriction enzymes that cleave the newly generated recognition sequence
AasI	GACNNNN^NNGTC	GACNNNNGTC	BoxI
AdeI, FastDigest® AdeI	CACNNN^GTG	CACGTG	Eco72I, MaeII, Ppu21I, TaiI
BglI, FastDigest® BglI	GCCNNNN^NGGC	GCCNNGGC	BseDI
Bsh1285I	CGRY^CG	CGCG	Bsh1236I
CaiI	CAGNNN^CTG	CAGCTG	AluI, CviJI, MspA1I, PvuII, FastDigest® PvuII
Cfr42I	CCGC^GG	CCGG	HpaII, FastDigest® MspI, MspI
Eam1105I	GACNNN^NNGTC	GACNNNNGTC	BoxI
FseI	GGCCGG^CC	GGCC	[BsuRI], [FastDigest® BsuRI], [CviJI]
Hpy188I	TCN^GA	TCGA	TaqI, FastDigest® TaqI
PacI	TTAAT^TAA	TTATAA	PsiI
PvuI, FastDigest® PvuI	CGAT^CG	CGCG	Bsh1236I
SdaI, FastDigest® SdaI	CCTGCA^GG	CCGG	HpaII, FastDigest® MspI, MspI
SfiI	GGCCNNNN^NGGCC	GGCCNNGGCC	BseDI, [BsuRI], [FastDigest® BsuRI], [CviJI]
SgfI	GCGAT^CGC	GCGCGC	Bsh1236I, Cac8I, FastDigest® HhaI, HhaI, Hin6I, PauI
TaaI	ACN^GT	ACGT	MaeII, TaiI

catalog@fermentas.com

Updated sausio 31, 2008 08:51