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Newly Generated Recognition Sequences Resulting from:

• Ligation of Blunt DNA Ends

• Ligation of Protruding Compatible DNA Ends
• Fill-in of a 5'-overhang and Self-ligation (see below)
• Removal of a 3'-overhang and Self-ligation

Newly Generated Recognition Sequences Resulting from the Fill-in of a 5'-overhang and Self-ligation (M--Z)

A-B-C-D-E-F-G-H-I-K-L    M-N-O-P-R-S-T-V-X-Z

Note

• Restriction enzymes that have degenerate recognition sequences (i.e., recognize more than one sequence) are indicated by an asterisk (*). Be aware that these restriction enzymes will cleave sequences in addition to the one listed.
• [] denotes the enzymes that cleave the target both before and after the ligation.
• Enzymes produced by Fermentas are shown in green.

Single letter code:
R = G or A;  Y = C or T;  W = A or T;  M = A or C;	K = G or T;  S = C or G; H = A, C or T; V = A, C or G;	B = C, G or T; D = A, G or T; N = G, A, T or C.

Restriction enzyme	Recognition sequence	Newly generated sequence after reaction	Restriction enzymes that cleave the newly generated recognition sequence
MaeII	A^CGT	ACGCGT	AflIII, Bsh1236I, MluI, FastDigest® MluI
MaeIII	^GTNAC	GTNACGTNAC	MaeII, [MaeIII], TaiI
MaeIII*	^GTAAC	GTAACGTAAC	MaeII, [MaeIII], TaiI
MaeIII*	^GTCAC	GTCACGTCAC	AjiI, MaeII, [MaeIII], [NmuCI], [FastDigest® NmuCI], TaiI
MaeIII*	^GTGAC	GTGACGTGAC	MaeII, [MaeIII], [NmuCI], [FastDigest® NmuCI], TaiI
MaeIII*	^GTTAC	GTTACGTTAC	MaeII, [MaeIII], TaiI
MboI	^GATC	GATCGATC	[Bsp143I], FastDigest® Bsu15I, Bsu15I, [DpnI], [MboI], TaqI, FastDigest® TaqI
MluI, FastDigest® MluI	A^CGCGT	ACGCGCGCGT	[Bsh1236I], Cac8I, FastDigest® HhaI, HhaI, Hin6I, PauI
MspI, FastDigest® MspI	C^CGG	CCGCGG	BseDI, Bsh1236I, BtgI, Cfr42I, MspA1I, SsiI
MunI, FastDigest® MunI	C^AATTG	CAATTAATTG	[TasI], Tru1I, FastDigest® VspI, VspI
MvaI, FastDigest® MvaI	CC^WGG	CCWWGG	BseDI, FastDigest® Eco130I, Eco130I
MvaI*, FastDigest® MvaI*	CC^AGG	CCAAGG	BseDI, FastDigest® Eco130I, Eco130I
MvaI*, FastDigest® MvaI*	CC^TGG	CCTTGG	BseDI, FastDigest® Eco130I, Eco130I
NarI	GG^CGCC	GGCGCGCC	Bsh1236I, Cac8I, [FastDigest® HhaI], [HhaI], [Hin6I], PauI, SgsI
NcoI, FastDigest® NcoI	C^CATGG	CCATGCATGG	[CviAII], [FatI], [Hin1II], HpyCH4V, FastDigest® Mph1103I, Mph1103I
NgoMIV	G^CCGGC	GCCGGCCGGC	Bsh1285I, BsuRI, FastDigest® BsuRI, [Cac8I], [FastDigest® Cfr10I], [Cfr10I], CfrI, CfrI, CviJI, Eco52I, [HpaII], [FastDigest® MspI], [MspI], [NgoMIV], [PdiI]
NheI	G^CTAGC	GCTAGCTAGC	AluI, [BmtI], [Cac8I], CviJI, [FspBI], [FastDigest® FspBI], [NheI]
NmuCI, FastDigest® NmuCI	^GTSAC	GTSACGTSAC	MaeII, [MaeIII], [NmuCI], [FastDigest® NmuCI], TaiI
NmuCI*, FastDigest® NmuCI*	^GTCAC	GTCACGTCAC	AjiI, MaeII, [MaeIII], [NmuCI], [FastDigest® NmuCI], TaiI
NmuCI*, FastDigest® NmuCI*	^GTGAC	GTGACGTGAC	MaeII, [MaeIII], [NmuCI], [FastDigest® NmuCI], TaiI
NotI, FastDigest® NotI	GC^GGCCGC	GCGGCCGGCCGC	[Bsh1285I], [BsuRI], [FastDigest® BsuRI], Cac8I, FastDigest® Cfr10I, Cfr10I, [CfrI], [CfrI], [CviJI], [Eco52I], FseI, HpaII, FastDigest® MspI, MspI, NgoMIV, PdiI, [SatI], [SsiI], [TauI]
PagI	T^CATGA	TCATGCATGA	[CviAII], [FatI], [Hin1II], HpyCH4V, FastDigest® Mph1103I, Mph1103I
PasI*	CC^CAGGG	CCCAGCAGGG	BseYI, EcoP15I
PauI	G^CGCGC	GCGCGCGCGC	[Bsh1236I], [Cac8I], [FastDigest® HhaI], [HhaI], [Hin6I], [PauI]
Pfl23II	C^GTACG	CGTACGTACG	[Csp6I], FastDigest® Eco105I, Eco105I, MaeII, [Pfl23II], Ppu21I, [FastDigest® RsaI], [RsaI], TaiI
PfoI	T^CCNGGA	TCCNGGCCNGGA	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI
PfoI*	T^CCAGGA	TCCAGGCCAGGA	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
PfoI*	T^CCCGGA	TCCCGGCCCGGA	[AcoI], [FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], BmgT120I, [BssKI], BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, [HpaII], [FastDigest® MspI], [MspI]
PfoI*	T^CCGGGA	TCCGGGCCGGGA	[AcoI], [FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], BmgT120I, [BssKI], BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, [HpaII], [FastDigest® MspI], [MspI]
PfoI*	T^CCTGGA	TCCTGGCCTGGA	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
PscI	A^CATGT	ACATGCATGT	[CviAII], [FatI], [Hin1II], HpyCH4V, FastDigest® Mph1103I, Mph1103I, [XceI]
Psp1406I, FastDigest® Psp1406I	AA^CGTT	AACGCGTT	AflIII, Bsh1236I, MluI, FastDigest® MluI
PspXI	VC^TCGAGB	VCTCGATCGAGB	Bsh1285I, Bsp143I, DpnI, MboI, PvuI, FastDigest® PvuI, [TaqI], [FastDigest® TaqI]
PsuI	R^GATCY	RGATCGATCY	[Bsp143I], FastDigest® Bsu15I, Bsu15I, [DpnI], [MboI], TaqI, FastDigest® TaqI
PsyI	GACN^NNGTC	GACNNNNGTC	BoxI
PsyI*	GACN^ANGTC	GACNAANGTC	BoxI
PsyI*	GACN^CNGTC	GACNCCNGTC	BoxI
PsyI*	GACN^GNGTC	GACNGGNGTC	BoxI
PsyI*	GACN^TNGTC	GACNTTNGTC	BoxI
SalI, FastDigest® SalI	G^TCGAC	GTCGATCGAC	Bsh1285I, Bsp143I, DpnI, MboI, PvuI, FastDigest® PvuI, [TaqI], [FastDigest® TaqI]
SanDI*	GG^GACCC	GGGACGACCC	[FaqI]
SatI	GC^NGC	GCNNGC	Cac8I
SatI*	GC^AGC	GCAAGC	Cac8I
SatI*	GC^CGC	GCCCGC	Cac8I, SmuI, [SsiI]
SatI*	GC^GGC	GCGGGC	Cac8I
SatI*	GC^TGC	GCTTGC	Cac8I
SexAI	A^CCWGGT	ACCWGGCCWGGT	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
SexAI*	A^CCAGGT	ACCAGGCCAGGT	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
SexAI*	A^CCTGGT	ACCTGGCCTGGT	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
SgrAI	CR^CCGGYG	CRCCGGCCGGYG	Bsh1285I, BsuRI, FastDigest® BsuRI, [FastDigest® Cfr10I], [Cfr10I], CfrI, CfrI, CviJI, Eco52I, [HpaII], [FastDigest® MspI], [MspI]
SgsI	GG^CGCGCC	GGCGCGCGCGCC	[Bsh1236I], [Cac8I], [FastDigest® HhaI], [HhaI], [Hin6I], [PauI]
SmoI*	C^TCGAG	CTCGATCGAG	Bsh1285I, Bsp143I, DpnI, MboI, PvuI, FastDigest® PvuI, [TaqI], [FastDigest® TaqI]
SmoI*	C^TTAAG	CTTAATTAAG	PacI, TasI, [Tru1I]
SsiI	C^CGC	CCGCGC	Bsh1236I, FastDigest® HhaI, HhaI, Hin6I, [SsiI]
TaqI, FastDigest® TaqI	T^CGA	TCGCGA	Bsh1236I, Bsp68I, Hpy188III
TasI	^AATT	AATTAATT	[TasI], Tru1I, FastDigest® VspI, VspI
TatI	W^GTACW	WGTACGTACW	[Csp6I], FastDigest® Eco105I, Eco105I, MaeII, Ppu21I, [FastDigest® RsaI], [RsaI], TaiI
Tru1I	T^TAA	TTATAA	PsiI
TseI	G^CWGC	GCWGCWGC	[SatI], [TseI]
TseI*	G^CAGC	GCAGCAGC	[BseXI], EcoP15I, [SatI], [TseI]
TseI*	G^CTGC	GCTGCTGC	[SatI], [TseI]
VspI, FastDigest® VspI	AT^TAAT	ATTATAAT	PsiI
XapI, FastDigest® XapI	R^AATTY	RAATTAATTY	[TasI], Tru1I, FastDigest® VspI, VspI
XbaI, FastDigest® XbaI	T^CTAGA	TCTAGCTAGA	AluI, CviJI, [FspBI], [FastDigest® FspBI]
XhoI, FastDigest® XhoI	C^TCGAG	CTCGATCGAG	Bsh1285I, Bsp143I, DpnI, MboI, PvuI, FastDigest® PvuI, [TaqI], [FastDigest® TaqI]
XmaJI, FastDigest® XmaJI	C^CTAGG	CCTAGCTAGG	AluI, CviJI, [FspBI], [FastDigest® FspBI]
XmiI*, FastDigest® XmiI*	GT^CGAC	GTCGCGAC	Bsh1236I, Bsp68I, Hpy188III
XmiI*, FastDigest® XmiI*	GT^CTAC	GTCTCTAC	FastDigest® Alw26I, Alw26I

catalog@fermentas.com

Updated sausio 31, 2008 08:51