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Newly Generated Recognition Sequences Resulting from:

• Ligation of Blunt DNA Ends

• Ligation of Protruding Compatible DNA Ends
• Fill-in of a 5'-overhang and Self-ligation (see below)
• Removal of a 3'-overhang and Self-ligation

Newly Generated Recognition Sequences Resulting from the Fill-in of a 5'-overhang and Self-ligation (A--L)

A-B-C-D-E-F-G-H-I-K-L    M-N-O-P-R-S-T-V-X-Z    

Note

• Restriction enzymes that have degenerate recognition sequences (i.e., recognize more than one sequence) are indicated by an asterisk (*). Be aware that these restriction enzymes will cleave sequences in addition to the one listed.
• [] denotes the enzymes that cleave the target both before and after the ligation.
• Enzymes produced by Fermentas are shown in green.

Single letter code:
R = G or A;  Y = C or T;  W = A or T;  M = A or C;	K = G or T;  S = C or G; H = A, C or T; V = A, C or G;	B = C, G or T; D = A, G or T; N = G, A, T or C.

Restriction enzyme	Recognition sequence	Newly generated sequence after reaction	Restriction enzymes that cleave the newly generated recognition sequence
Acc65I	G^GTACC	GGTACGTACC	[Csp6I], FastDigest® Eco105I, Eco105I, MaeII, Ppu21I, [FastDigest® RsaI], [RsaI], TaiI
AcoI	Y^CCGGR	YCCGGCCGGR	Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I, [HpaII], [FastDigest® MspI], [MspI]
AflIII*	A^CACGT	ACACGCACGT	[MaeII], [TaiI]
AflIII*	A^CATGT	ACATGCATGT	[CviAII], [FatI], [Hin1II], HpyCH4V, FastDigest® Mph1103I, Mph1103I, [XceI]
AflIII*	A^CGCGT	ACGCGCGCGT	[Bsh1236I], Cac8I, FastDigest® HhaI, HhaI, Hin6I, PauI
AflIII*	A^CGTGT	ACGTGCGTGT	[MaeII], [TaiI]
Alw44I	G^TGCAC	GTGCATGCAC	Cac8I, CviAII, FatI, Hin1II, [HpyCH4V], PaeI, FastDigest® PaeI, XceI
BamHI, FastDigest® BamHI	G^GATCC	GGATCGATCC	[Bsp143I], [BspPI], FastDigest® Bsu15I, Bsu15I, [DpnI], [MboI], TaqI, FastDigest® TaqI
BbvCI	CC^TCAGC	CCTCATCAGC	[MnlI]
BclI	T^GATCA	TGATCGATCA	[Bsp143I], FastDigest® Bsu15I, Bsu15I, [DpnI], [MboI], TaqI, FastDigest® TaqI
BcnI, FastDigest® BcnI	CC^SGG	CCSSGG	BseDI
BcnI*, FastDigest® BcnI*	CC^CGG	CCCCGG	[FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], BseDI, [BssKI], [HpaII], [FastDigest® MspI], [MspI]
BcnI*, FastDigest® BcnI*	CC^GGG	CCGGGG	[FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], BseDI, [BssKI], [HpaII], [FastDigest® MspI], [MspI]
BcuI, FastDigest® BcuI	A^CTAGT	ACTAGCTAGT	AluI, CviJI, [FspBI], [FastDigest® FspBI]
BfmI*	C^TGCAG	CTGCATGCAG	Cac8I, CviAII, FatI, Hin1II, [HpyCH4V], PaeI, FastDigest® PaeI, XceI
BglII, FastDigest® BglII	A^GATCT	AGATCGATCT	[Bsp143I], FastDigest® Bsu15I, Bsu15I, [DpnI], [MboI], TaqI, FastDigest® TaqI
Bme1390I, FastDigest® Bme1390I	CC^NGG	CCNNGG	BseDI
Bme1390I*, FastDigest® Bme1390I*	CC^AGG	CCAAGG	BseDI, FastDigest® Eco130I, Eco130I
Bme1390I*, FastDigest® Bme1390I*	CC^CGG	CCCCGG	[FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], BseDI, [BssKI], [HpaII], [FastDigest® MspI], [MspI]
Bme1390I*, FastDigest® Bme1390I*	CC^GGG	CCGGGG	[FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], BseDI, [BssKI], [HpaII], [FastDigest® MspI], [MspI]
Bme1390I*, FastDigest® Bme1390I*	CC^TGG	CCTTGG	BseDI, FastDigest® Eco130I, Eco130I
BmeT110I	CY^CGRG	CYCGCGRG	Bsh1236I
BmgT120I	GG^NCC	GGNNCC	FastDigest® BspLI, BspLI
BmgT120I*	GG^ACC	GGAACC	FastDigest® BspLI, BspLI
BmgT120I*	GG^CCC	GGCCCC	[BmgT120I], FastDigest® BspLI, BspLI, [BsuRI], [FastDigest® BsuRI], [Cfr13I], [FastDigest® Cfr13I], [CviJI]
BmgT120I*	GG^GCC	GGGGCC	[BmgT120I], FastDigest® BspLI, BspLI, [BsuRI], [FastDigest® BsuRI], [Cfr13I], [FastDigest® Cfr13I], [CviJI]
BmgT120I*	GG^TCC	GGTTCC	FastDigest® BspLI, BspLI
Bpu10I*	CC^TCAGC	CCTCATCAGC	[MnlI]
BsaWI	W^CCGGW	WCCGGCCGGW	Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I, [HpaII], [FastDigest® MspI], [MspI]
BseYI	C^CCAGC	CCCAGCCAGC	[BseYI], Cac8I, CviJI
BshNI*	G^GCACC	GGCACGCACC	Cac8I
BshNI*	G^GCGCC	GGCGCGCGCC	Bsh1236I, Cac8I, [FastDigest® HhaI], [HhaI], [Hin6I], PauI
BshNI*	G^GTACC	GGTACGTACC	[Csp6I], FastDigest® Eco105I, Eco105I, MaeII, Ppu21I, [FastDigest® RsaI], [RsaI], TaiI
BshNI*	G^GTGCC	GGTGCGTGCC	Cac8I
BshTI, FastDigest® BshTI	A^CCGGT	ACCGGCCGGT	Bsh1285I, BsuRI, FastDigest® BsuRI, [FastDigest® Cfr10I], [Cfr10I], CfrI, CfrI, CviJI, Eco52I, [HpaII], [FastDigest® MspI], [MspI]
Bsp119I, FastDigest® Bsp119I	TT^CGAA	TTCGCGAA	Bsh1236I, Bsp68I, Hpy188III
Bsp120I	G^GGCCC	GGGCCGGCCC	[BmgT120I], [BsuRI], [FastDigest® BsuRI], Cac8I, FastDigest® Cfr10I, Cfr10I, [Cfr13I], [FastDigest® Cfr13I], [CviJI], FseI, HpaII, FastDigest® MspI, MspI, NgoMIV, PdiI
Bsp1407I, FastDigest® Bsp1407I	T^GTACA	TGTACGTACA	[Csp6I], FastDigest® Eco105I, Eco105I, MaeII, Ppu21I, [FastDigest® RsaI], [RsaI], TaiI
Bsp143I	^GATC	GATCGATC	[Bsp143I], FastDigest® Bsu15I, Bsu15I, [DpnI], [MboI], TaqI, FastDigest® TaqI
BspTI	C^TTAAG	CTTAATTAAG	PacI, TasI, [Tru1I]
BssKI	^CCNGG	CCNGGCCNGG	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI
BssKI*	^CCAGG	CCAGGCCAGG	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
BssKI*	^CCCGG	CCCGGCCCGG	[FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], BmgT120I, [BssKI], BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, [HpaII], [FastDigest® MspI], [MspI]
BssKI*	^CCGGG	CCGGGCCGGG	[FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], BmgT120I, [BssKI], BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, [HpaII], [FastDigest® MspI], [MspI]
BssKI*	^CCTGG	CCTGGCCTGG	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
Bsu15I, FastDigest® Bsu15I	AT^CGAT	ATCGCGAT	Bsh1236I, Bsp68I, Hpy188III
BtgI*	C^CATGG	CCATGCATGG	[CviAII], [FatI], [Hin1II], HpyCH4V, FastDigest® Mph1103I, Mph1103I
BtgI*	C^CGCGG	CCGCGCGCGG	[Bsh1236I], Cac8I, FastDigest® HhaI, HhaI, Hin6I, PauI, [SsiI]
Cfr10I, FastDigest® Cfr10I	R^CCGGY	RCCGGCCGGY	Bsh1285I, BsuRI, FastDigest® BsuRI, [FastDigest® Cfr10I], [Cfr10I], CfrI, CfrI, CviJI, Eco52I, [HpaII], [FastDigest® MspI], [MspI]
Cfr13I*, FastDigest® Cfr13I*	G^GCCC	GGCCGCCC	[BsuRI], [FastDigest® BsuRI], [CviJI], SatI, SsiI, TauI
Cfr13I*, FastDigest® Cfr13I*	G^GGCC	GGGCGGCC	[BsuRI], [FastDigest® BsuRI], [CviJI], SatI, TauI
Cfr9I	C^CCGGG	CCCGGCCGGG	[FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], Bsh1285I, [BssKI], BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I, [HpaII], [FastDigest® MspI], [MspI]
CfrI	Y^GGCCR	YGGCCGGCCR	[BsuRI], [FastDigest® BsuRI], Cac8I, FastDigest® Cfr10I, Cfr10I, [CfrI], [CfrI], [CviJI], FseI, HpaII, FastDigest® MspI, MspI, NgoMIV, PdiI
CfrI	Y^GGCCR	YGGCCGGCCR	[BsuRI], [FastDigest® BsuRI], Cac8I, FastDigest® Cfr10I, Cfr10I, [CfrI], [CfrI], [CviJI], FseI, HpaII, FastDigest® MspI, MspI, NgoMIV, PdiI
CpoI*	CG^GACCG	CGGACGACCG	Bsh1285I
CpoI*	CG^GTCCG	CGGTCGTCCG	Bsh1285I
Csp6I	G^TAC	GTATAC	Bst1107I, FastDigest® Bst1107I, FastDigest® Hpy8I, Hpy8I, FastDigest® XmiI, XmiI
CviAII	C^ATG	CATATG	FastDigest® NdeI, NdeI
Eco130I*, FastDigest® Eco130I*	C^CATGG	CCATGCATGG	[CviAII], [FatI], [Hin1II], HpyCH4V, FastDigest® Mph1103I, Mph1103I
Eco130I*, FastDigest® Eco130I*	C^CTAGG	CCTAGCTAGG	AluI, CviJI, [FspBI], [FastDigest® FspBI]
Eco52I	C^GGCCG	CGGCCGGCCG	[Bsh1285I], [BsuRI], [FastDigest® BsuRI], Cac8I, FastDigest® Cfr10I, Cfr10I, [CfrI], [CfrI], [CviJI], [Eco52I], FseI, HpaII, FastDigest® MspI, MspI, NgoMIV, PdiI
Eco81I*	CC^TCAGG	CCTCATCAGG	[MnlI]
Eco88I, FastDigest® Eco88I	C^YCGRG	CYCGRYCGRG	Bsh1285I
Eco88I*, FastDigest® Eco88I*	C^CCGAG	CCCGACCGAG	Bsh1285I, TaqII
Eco88I*, FastDigest® Eco88I*	C^CCGGG	CCCGGCCGGG	[FastDigest® BcnI], [BcnI], [Bme1390I], [FastDigest® Bme1390I], Bsh1285I, [BssKI], BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I, [HpaII], [FastDigest® MspI], [MspI]
Eco88I*, FastDigest® Eco88I*	C^TCGAG	CTCGATCGAG	Bsh1285I, Bsp143I, DpnI, MboI, PvuI, FastDigest® PvuI, [TaqI], [FastDigest® TaqI]
Eco88I*, FastDigest® Eco88I*	C^TCGGG	CTCGGTCGGG	Bsh1285I
Eco91I	G^GTNACC	GGTNACGTNACC	MaeII, [MaeIII], TaiI
Eco91I*	G^GTAACC	GGTAACGTAACC	MaeII, [MaeIII], TaiI
Eco91I*	G^GTCACC	GGTCACGTCACC	AjiI, MaeII, [MaeIII], [NmuCI], [FastDigest® NmuCI], TaiI
Eco91I*	G^GTGACC	GGTGACGTGACC	[HphI], MaeII, [MaeIII], [NmuCI], [FastDigest® NmuCI], TaiI
Eco91I*	G^GTTACC	GGTTACGTTACC	MaeII, [MaeIII], TaiI
EcoO109I*	RG^GCCCY	RGGCCGCCCY	[BsuRI], [FastDigest® BsuRI], [CviJI], SatI, SsiI, TauI
EcoO109I*	RG^GGCCY	RGGGCGGCCY	[BsuRI], [FastDigest® BsuRI], [CviJI], SatI, TauI
EcoRI, FastDigest® EcoRI	G^AATTC	GAATTAATTC	PdmI, [TasI], Tru1I, FastDigest® VspI, VspI
EcoRII	^CCWGG	CCWGGCCWGG	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
EcoRII*	^CCAGG	CCAGGCCAGG	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
EcoRII*	^CCTGG	CCTGGCCTGG	[Bme1390I], [FastDigest® Bme1390I], [BssKI], BsuRI, FastDigest® BsuRI, CviJI, [EcoRII], [FastDigest® MvaI], [MvaI]
FatI	^CATG	CATGCATG	[CviAII], [FatI], [Hin1II], HpyCH4V, FastDigest® Mph1103I, Mph1103I
FspBI, FastDigest® FspBI	C^TAG	CTATAG	BfmI
Hin1I	GR^CGYC	GRCGCGYC	Bsh1236I
Hin6I	G^CGC	GCGCGC	Bsh1236I, Cac8I, [FastDigest® HhaI], [HhaI], [Hin6I], PauI
HindIII, FastDigest® HindIII	A^AGCTT	AAGCTAGCTT	[AluI], BmtI, Cac8I, [CviJI], FspBI, FastDigest® FspBI, NheI
HpaII	C^CGG	CCGCGG	BseDI, Bsh1236I, BtgI, Cfr42I, MspA1I, SsiI
KasI	G^GCGCC	GGCGCGCGCC	Bsh1236I, Cac8I, [FastDigest® HhaI], [HhaI], [Hin6I], PauI
Kpn2I, FastDigest® Kpn2I	T^CCGGA	TCCGGCCGGA	Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I, [HpaII], [FastDigest® MspI], [MspI]

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Updated sausio 31, 2008 08:51