Newly Generated Recognition Sequences Resulting from:
- Ligation of Protruding Compatible DNA Ends (see below)
- Fill-in of a 5'-overhang and Self-ligation
- Removal of a 3'-overhang and Self-ligation
Newly Generated Recognition Sequences Resulting from the Ligation of Protruding Compatible DNA Ends (S--Z)
A-B C-D-E-F-G-H-I-K-L M-N-O-P-R S-T-V-X-Z
Note
- Restriction enzymes that have degenerate recognition sequences (i.e., recognize more than one sequence) are indicated by an asterisk (*). Be aware that these restriction enzymes will cleave sequences in addition to the one listed.
- Enzymes produced by Fermentas are shown in green.
Single letter code: R = G or A;
Y = C or T;
W = A or T;
M = A or C;K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;B = C, G or T;
D = A, G or T;
N = G, A, T or C.
First restriction
enzymeSecond restriction
enzymeRestriction enzymes that cleave
the newly generated recognition sequenceSacI (GAGCT^C),
FastDigest® SacI
(GAGCT^C)Alw21I* (GAGCT^C), Eco24I* (GAGCT^C), SduI* (GAGCT^C) AluI, Alw21I, CviJI, Ecl136II, Eco24I, FastDigest® SacI, SacI, SduI SalI (G^TCGAC),
FastDigest® SalI
(G^TCGAC)Eco88I* (C^TCGAG), FastDigest® Eco88I* (C^TCGAG), PspXI* (VC^TCGAGC), PspXI* (VC^TCGAGG), PspXI* (VC^TCGAGT), SmoI* (C^TCGAG), FastDigest® XhoI (C^TCGAG), XhoI (C^TCGAG) TaqI, FastDigest® TaqI SanDI* (GG^GACCC) CpoI* (CG^GACCG), Eco47I* (G^GACC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, FaqI Psp5II* (RG^GACCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, EcoO109I, FaqI, Psp5II, SanDI Psp5II* (RG^GACCT) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, EcoO109I, FaqI, Psp5II SanDI* (GG^GTCCC) CpoI* (CG^GTCCG), Eco47I* (G^GTCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I Psp5II* (RG^GTCCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, EcoO109I, Psp5II, SanDI Psp5II* (RG^GTCCT) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, EcoO109I, Psp5II SdaI (CCTGCA^GG),
FastDigest® SdaI
(CCTGCA^GG)Alw21I* (GTGCA^C), BseSI* (GTGCA^C), FastDigest® Mph1103I (ATGCA^T), Mph1103I (ATGCA^T), SduI* (GTGCA^C) HpyCH4V PstI (CTGCA^G), FastDigest® PstI (CTGCA^G) BfmI, HpyCH4V, PstI, FastDigest® PstI SduI* (GAGCA^C) Alw21I* (GAGCA^C) Alw21I, SduI SduI* (GAGCC^C) Eco24I* (GAGCC^C) CviJI, Eco24I, SduI SduI* (GAGCT^C) Alw21I* (GAGCT^C), Eco24I* (GAGCT^C), FastDigest® SacI (GAGCT^C), SacI (GAGCT^C) AluI, Alw21I, CviJI, Ecl136II, Eco24I, FastDigest® SacI, SacI, SduI SduI* (GGGCA^C) BseSI* (GGGCA^C) BseSI, SduI SduI* (GGGCC^C) ApaI (GGGCC^C), FastDigest® ApaI (GGGCC^C), BseSI* (GGGCC^C), Eco24I* (GGGCC^C) ApaI, FastDigest® ApaI, BmgT120I, BseSI, Bsp120I, FastDigest® BspLI, BspLI, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, Eco24I, SduI SduI* (GGGCT^C) Eco24I* (GGGCT^C) CviJI, Eco24I, SduI SduI* (GTGCA^C) Alw21I* (GTGCA^C), BseSI* (GTGCA^C) Alw21I, Alw44I, BseSI, FastDigest® Hpy8I, Hpy8I, HpyCH4V, SduI FastDigest® Mph1103I (ATGCA^T), Mph1103I (ATGCA^T) HpyCH4V PstI (CTGCA^G), FastDigest® PstI (CTGCA^G), FastDigest® SdaI (CCTGCA^GG), SdaI (CCTGCA^GG) BsgI, HpyCH4V SduI* (GTGCC^C) BseSI* (GTGCC^C) BseSI, SduI SduI* (GTGCT^C) Alw21I* (GTGCT^C) Alw21I, SduI SexAI* (A^CCAGGT) EcoRII* (^CCAGG) Bme1390I, FastDigest® Bme1390I, BssKI, EcoRII, FastDigest® MvaI, MvaI SexAI* (A^CCTGGT) EcoRII* (^CCTGG) Bme1390I, FastDigest® Bme1390I, BssKI, EcoRII, FastDigest® MvaI, MvaI SgfI (GCGAT^CGC) Bsh1285I* (CGAT^CG), PvuI (CGAT^CG), FastDigest® PvuI (CGAT^CG) Bsh1285I, Bsp143I, DpnI, MboI, PvuI, FastDigest® PvuI PacI (TTAAT^TAA) Tru1I SgrAI* (CA^CCGGYG) AcoI* (Y^CCGGA), BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) BsaWI, HpaII, FastDigest® MspI, MspI AcoI* (Y^CCGGG), Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGT) BsaWI, FastDigest® BshTI, BshTI, FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI FastDigest® Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGC), NgoMIV (G^CCGGC) FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI SgrAI* (CG^CCGGYG) AcoI* (Y^CCGGA), BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) HpaII, FastDigest® MspI, MspI AcoI* (Y^CCGGG), Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGT) FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI FastDigest® Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGC), NgoMIV (G^CCGGC) Cac8I, FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI, NgoMIV, PdiI SgsI (GG^CGCGCC) AflIII* (A^CGCGT), BtgI* (C^CGCGG), MluI (A^CGCGT), FastDigest® MluI (A^CGCGT) Bsh1236I, FastDigest® HhaI, HhaI, Hin6I PauI (G^CGCGC) Bsh1236I, Cac8I, FastDigest® HhaI, HhaI, Hin6I, PauI SmoI* (C^TCGAG) Eco88I* (C^TCGAG), FastDigest® Eco88I* (C^TCGAG), PspXI* (VC^TCGAGC), PspXI* (VC^TCGAGG), PspXI* (VC^TCGAGT), FastDigest® XhoI (C^TCGAG), XhoI (C^TCGAG) BmeT110I, Eco88I, FastDigest® Eco88I, SmoI, TaqI, FastDigest® TaqI, FastDigest® XhoI, XhoI SalI (G^TCGAC), FastDigest® SalI (G^TCGAC) TaqI, FastDigest® TaqI SmoI* (C^TTAAG) BspTI (C^TTAAG) BspTI, SmoI, Tru1I SsiI* (C^CGC) BmeT110I* (CY^CGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI Hin1I* (GR^CGCC), Hin6I (G^CGC), NarI (GG^CGCC) SsiI HpaII (C^CGG), FastDigest® MspI (C^CGG), MspI (C^CGG) HpaII, FastDigest® MspI, MspI SsiI* (G^CGG) Hin1I* (GR^CGCC), Hin6I (G^CGC), NarI (GG^CGCC) FastDigest® HhaI, HhaI, Hin6I TaiI (ACGT) AatII (GACGT^C) MaeII, TaiI TaqI (T^CGA),
FastDigest® TaqI
(T^CGA)BmeT110I* (CY^CGAG), Bsp119I (TT^CGAA), FastDigest® Bsp119I (TT^CGAA), FastDigest® Bsu15I (AT^CGAT), Bsu15I (AT^CGAT), FastDigest® XmiI* (GT^CGAC), XmiI* (GT^CGAC) TaqI, FastDigest® TaqI TasI (^AATT) FastDigest® EcoRI (G^AATTC), EcoRI (G^AATTC), FastDigest® MunI (C^AATTG), MunI (C^AATTG), XapI* (R^AATTC), XapI* (R^AATTT), FastDigest® XapI* (R^AATTC), FastDigest® XapI* (R^AATTT) TasI TatI* (A^GTACW) Acc65I (G^GTACC), BshNI* (G^GTACC), Pfl23II (C^GTACG) Csp6I, FastDigest® RsaI, RsaI Bsp1407I (T^GTACA), FastDigest® Bsp1407I (T^GTACA) Csp6I, FastDigest® RsaI, RsaI, TatI TatI* (T^GTACW) Acc65I (G^GTACC), BshNI* (G^GTACC), Pfl23II (C^GTACG) Csp6I, FastDigest® RsaI, RsaI Bsp1407I (T^GTACA), FastDigest® Bsp1407I (T^GTACA) Bsp1407I, FastDigest® Bsp1407I, Csp6I, FastDigest® RsaI, RsaI, TatI Tru1I (T^TAA) FastDigest® VspI (AT^TAAT), VspI (AT^TAAT) Tru1I VspI (AT^TAAT),
FastDigest® VspI
(AT^TAAT)Tru1I (T^TAA) Tru1I XapI* (A^AATTY),
FastDigest® XapI*
(A^AATTY)FastDigest® EcoRI (G^AATTC), EcoRI (G^AATTC) TasI, XapI, FastDigest® XapI FastDigest® MunI (C^AATTG), MunI (C^AATTG), TasI (^AATT) TasI XapI* (G^AATTY),
FastDigest® XapI*
(G^AATTY)FastDigest® EcoRI (G^AATTC), EcoRI (G^AATTC) FastDigest® EcoRI, EcoRI, TasI, XapI, FastDigest® XapI FastDigest® MunI (C^AATTG), MunI (C^AATTG), TasI (^AATT) TasI XbaI (T^CTAGA),
FastDigest® XbaI
(T^CTAGA)BcuI (A^CTAGT), FastDigest® BcuI (A^CTAGT), FastDigest® Eco130I* (C^CTAGG), Eco130I* (C^CTAGG), NheI (G^CTAGC), XmaJI (C^CTAGG), FastDigest® XmaJI (C^CTAGG) FspBI, FastDigest® FspBI XceI* (ACATG^Y) Hin1II (CATG) CviAII, FatI, Hin1II PaeI (GCATG^C), FastDigest® PaeI (GCATG^C) CviAII, FatI, Hin1II, XceI XceI* (GCATG^Y) Hin1II (CATG) CviAII, FatI, Hin1II PaeI (GCATG^C), FastDigest® PaeI (GCATG^C) Cac8I, CviAII, FatI, Hin1II, PaeI, FastDigest® PaeI, XceI XhoI (C^TCGAG),
FastDigest® XhoI
(C^TCGAG)Eco88I* (C^TCGAG), FastDigest® Eco88I* (C^TCGAG), PspXI* (VC^TCGAGC), PspXI* (VC^TCGAGG), PspXI* (VC^TCGAGT), SmoI* (C^TCGAG) BmeT110I, Eco88I, FastDigest® Eco88I, SmoI, TaqI, FastDigest® TaqI, FastDigest® XhoI, XhoI SalI (G^TCGAC), FastDigest® SalI (G^TCGAC) TaqI, FastDigest® TaqI XmaJI (C^CTAGG),
FastDigest® XmaJI
(C^CTAGG)BcuI (A^CTAGT), FastDigest® BcuI (A^CTAGT), NheI (G^CTAGC), FastDigest® XbaI (T^CTAGA), XbaI (T^CTAGA) FspBI, FastDigest® FspBI FastDigest® Eco130I* (C^CTAGG), Eco130I* (C^CTAGG) BseDI, FastDigest® Eco130I, Eco130I, FspBI, FastDigest® FspBI, XmaJI, FastDigest® XmaJI XmiI* (GT^CGAC),
FastDigest® XmiI*
(GT^CGAC)BmeT110I* (CY^CGAG), Bsp119I (TT^CGAA), FastDigest® Bsp119I (TT^CGAA), FastDigest® Bsu15I (AT^CGAT), Bsu15I (AT^CGAT), TaqI (T^CGA), FastDigest® TaqI (T^CGA) TaqI, FastDigest® TaqI
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Updated sausio 31, 2008 08:51
