Newly Generated Recognition Sequences Resulting from:
- Ligation of Protruding Compatible DNA Ends (see below)
- Fill-in of a 5'-overhang and Self-ligation
- Removal of a 3'-overhang and Self-ligation
Newly Generated Recognition Sequences Resulting from the Ligation of Protruding Compatible DNA Ends (M--R)
A-B C-D-E-F-G-H-I-K-L M-N-O-P-R S-T-V-X-Z
Note
- Restriction enzymes that have degenerate recognition sequences (i.e., recognize more than one sequence) are indicated by an asterisk (*). Be aware that these restriction enzymes will cleave sequences in addition to the one listed.
- Enzymes produced by Fermentas are shown in green.
Single letter code: R = G or A;
Y = C or T;
W = A or T;
M = A or C;K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;B = C, G or T;
D = A, G or T;
N = G, A, T or C.
First restriction
enzymeSecond restriction
enzymeRestriction enzymes that cleave
the newly generated recognition sequenceMaeII (A^CGT) Hin1I* (GR^CGTC), Psp1406I (AA^CGTT), FastDigest® Psp1406I (AA^CGTT) MaeII, TaiI MboI (^GATC) FastDigest® BamHI (G^GATCC), BamHI (G^GATCC), BclI (T^GATCA), BglII (A^GATCT), FastDigest® BglII (A^GATCT), Bsp143I (^GATC), PsuI* (R^GATCC), PsuI* (R^GATCT) Bsp143I, DpnI, MboI MluI (A^CGCGT),
FastDigest® MluI
(A^CGCGT)AflIII* (A^CGCGT) AflIII, Bsh1236I, MluI, FastDigest® MluI BtgI* (C^CGCGG) Bsh1236I PauI (G^CGCGC), SgsI (GG^CGCGCC) Bsh1236I, FastDigest® HhaI, HhaI, Hin6I Mph1103I (ATGCA^T),
FastDigest® Mph1103I
(ATGCA^T)Alw21I* (GTGCA^C), BseSI* (GTGCA^C), PstI (CTGCA^G), FastDigest® PstI (CTGCA^G), FastDigest® SdaI (CCTGCA^GG), SdaI (CCTGCA^GG), SduI* (GTGCA^C) HpyCH4V MspI (C^CGG),
FastDigest® MspI
(C^CGG)BmeT110I* (CY^CGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI Hin1I* (GR^CGCC), Hin6I (G^CGC), NarI (GG^CGCC), SsiI* (C^CGC) SsiI HpaII (C^CGG), SsiI* (G^CGG) HpaII, FastDigest® MspI, MspI MunI (C^AATTG),
FastDigest® MunI
(C^AATTG)FastDigest® EcoRI (G^AATTC), EcoRI (G^AATTC), TasI (^AATT), XapI* (R^AATTC), XapI* (R^AATTT), FastDigest® XapI* (R^AATTC), FastDigest® XapI* (R^AATTT) TasI NarI (GG^CGCC) Hin1I* (GR^CGCC) BbeI, BshNI, FastDigest® BspLI, BspLI, EheI, FastDigest® HhaI, HhaI, Hin1I, Hin6I, KasI, NarI Hin1I* (GR^CGTC) Hin1I Hin6I (G^CGC), SsiI* (C^CGC) FastDigest® HhaI, HhaI, Hin6I NcoI (C^CATGG),
FastDigest® NcoI
(C^CATGG)AflIII* (A^CATGT), FatI (^CATG), PagI (T^CATGA), PscI (A^CATGT) CviAII, FatI, Hin1II BtgI* (C^CATGG), FastDigest® Eco130I* (C^CATGG), Eco130I* (C^CATGG) BseDI, BtgI, CviAII, FastDigest® Eco130I, Eco130I, FatI, Hin1II, FastDigest® NcoI, NcoI NgoMIV (G^CCGGC) AcoI* (Y^CCGGA), BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) HpaII, FastDigest® MspI, MspI AcoI* (Y^CCGGG), Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGT), SgrAI* (CR^CCGGTG) FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI FastDigest® Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGC), SgrAI* (CR^CCGGCG) Cac8I, FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI, NgoMIV, PdiI NheI (G^CTAGC) BcuI (A^CTAGT), FastDigest® BcuI (A^CTAGT), FastDigest® Eco130I* (C^CTAGG), Eco130I* (C^CTAGG), FastDigest® XbaI (T^CTAGA), XbaI (T^CTAGA), XmaJI (C^CTAGG), FastDigest® XmaJI (C^CTAGG) FspBI, FastDigest® FspBI NotI (GC^GGCCGC),
FastDigest® NotI
(GC^GGCCGC)Bsp120I (G^GGCCC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, SatI, TauI CfrI* (Y^GGCCA), CfrI* (Y^GGCCA) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, SatI, TauI CfrI* (Y^GGCCG), CfrI* (Y^GGCCG), Eco52I (C^GGCCG) Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I, SatI, TauI PacI (TTAAT^TAA) Bsh1285I* (CGAT^CG), PvuI (CGAT^CG), FastDigest® PvuI (CGAT^CG), SgfI (GCGAT^CGC) Tru1I PaeI (GCATG^C),
FastDigest® PaeI
(GCATG^C)Hin1II (CATG) CviAII, FatI, Hin1II XceI* (RCATG^C) Cac8I, CviAII, FatI, Hin1II, PaeI, FastDigest® PaeI, XceI XceI* (RCATG^T) CviAII, FatI, Hin1II, XceI PagI (T^CATGA) AflIII* (A^CATGT), BtgI* (C^CATGG), FastDigest® Eco130I* (C^CATGG), Eco130I* (C^CATGG), FatI (^CATG), FastDigest® NcoI (C^CATGG), NcoI (C^CATGG), PscI (A^CATGT) CviAII, FatI, Hin1II PasI* (CC^CAGGG) TseI* (G^CAGC) BseYI PauI (G^CGCGC) AflIII* (A^CGCGT), BtgI* (C^CGCGG), MluI (A^CGCGT), FastDigest® MluI (A^CGCGT) Bsh1236I, FastDigest® HhaI, HhaI, Hin6I SgsI (GG^CGCGCC) Bsh1236I, Cac8I, FastDigest® HhaI, HhaI, Hin6I, PauI Pfl23II (C^GTACG) Acc65I (G^GTACC), BshNI* (G^GTACC), Bsp1407I (T^GTACA), FastDigest® Bsp1407I (T^GTACA), TatI* (W^GTACA), TatI* (W^GTACT) Csp6I, FastDigest® RsaI, RsaI PscI (A^CATGT) AflIII* (A^CATGT) AflIII, CviAII, FatI, Hin1II, PscI, XceI BtgI* (C^CATGG), FastDigest® Eco130I* (C^CATGG), Eco130I* (C^CATGG), FatI (^CATG), FastDigest® NcoI (C^CATGG), NcoI (C^CATGG), PagI (T^CATGA) CviAII, FatI, Hin1II Psp1406I (AA^CGTT),
FastDigest® Psp1406I
(AA^CGTT)Hin1I* (GR^CGTC), MaeII (A^CGT) MaeII, TaiI Psp5II* (AG^GACCY) CpoI* (CG^GACCG), Eco47I* (G^GACC) BmgT120I, Cfr13I, FastDigest® Cfr13I, Eco47I SanDI* (GG^GACCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, EcoO109I, Psp5II Psp5II* (AG^GTCCY) CpoI* (CG^GTCCG), Eco47I* (G^GTCC) BmgT120I, Cfr13I, FastDigest® Cfr13I, Eco47I SanDI* (GG^GTCCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, EcoO109I, Psp5II Psp5II* (GG^GACCY) CpoI* (CG^GACCG), Eco47I* (G^GACC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, FaqI SanDI* (GG^GACCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, EcoO109I, FaqI, Psp5II, SanDI Psp5II* (GG^GTCCY) CpoI* (CG^GTCCG), Eco47I* (G^GTCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I SanDI* (GG^GTCCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I, EcoO109I, Psp5II, SanDI PspXI* (AC^TCGAGB) Eco88I* (C^TCGAG), FastDigest® Eco88I* (C^TCGAG), SmoI* (C^TCGAG), FastDigest® XhoI (C^TCGAG), XhoI (C^TCGAG) BmeT110I, Eco88I, FastDigest® Eco88I, SmoI, TaqI, FastDigest® TaqI, FastDigest® XhoI, XhoI SalI (G^TCGAC), FastDigest® SalI (G^TCGAC) TaqI, FastDigest® TaqI PspXI* (CC^TCGAGB) Eco88I* (C^TCGAG), FastDigest® Eco88I* (C^TCGAG), SmoI* (C^TCGAG), FastDigest® XhoI (C^TCGAG), XhoI (C^TCGAG) BmeT110I, Eco88I, FastDigest® Eco88I, MnlI, SmoI, TaqI, FastDigest® TaqI, FastDigest® XhoI, XhoI SalI (G^TCGAC), FastDigest® SalI (G^TCGAC) MnlI, TaqI, FastDigest® TaqI PspXI* (GC^TCGAGB) Eco88I* (C^TCGAG), FastDigest® Eco88I* (C^TCGAG), SmoI* (C^TCGAG), FastDigest® XhoI (C^TCGAG), XhoI (C^TCGAG) BmeT110I, Eco88I, FastDigest® Eco88I, SmoI, TaqI, FastDigest® TaqI, FastDigest® XhoI, XhoI SalI (G^TCGAC), FastDigest® SalI (G^TCGAC) TaqI, FastDigest® TaqI PstI (CTGCA^G),
FastDigest® PstI
(CTGCA^G)Alw21I* (GTGCA^C), BseSI* (GTGCA^C), FastDigest® Mph1103I (ATGCA^T), Mph1103I (ATGCA^T), SduI* (GTGCA^C) HpyCH4V FastDigest® SdaI (CCTGCA^GG), SdaI (CCTGCA^GG) BfmI, HpyCH4V, PstI, FastDigest® PstI PsuI* (A^GATCY) FastDigest® BamHI (G^GATCC), BamHI (G^GATCC) Bsp143I, DpnI, MboI, PsuI BclI (T^GATCA), Bsp143I (^GATC), MboI (^GATC) Bsp143I, DpnI, MboI BglII (A^GATCT), FastDigest® BglII (A^GATCT) BglII, FastDigest® BglII, Bsp143I, DpnI, MboI, PsuI PsuI* (G^GATCY) FastDigest® BamHI (G^GATCC), BamHI (G^GATCC) FastDigest® BamHI, BamHI, Bsp143I, FastDigest® BspLI, BspLI, BspPI, DpnI, MboI, PsuI BclI (T^GATCA), Bsp143I (^GATC), MboI (^GATC) Bsp143I, BspPI, DpnI, MboI BglII (A^GATCT), FastDigest® BglII (A^GATCT) Bsp143I, BspPI, DpnI, MboI, PsuI PvuI (CGAT^CG),
FastDigest® PvuI
(CGAT^CG)Bsh1285I* (CGAT^CG), SgfI (GCGAT^CGC) Bsh1285I, Bsp143I, DpnI, MboI, PvuI, FastDigest® PvuI PacI (TTAAT^TAA) Tru1I
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Updated sausio 31, 2008 08:51
