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S U P P O R T
 
Newly Generated Recognition Sequences Resulting from:

Newly Generated Recognition Sequences Resulting from the Ligation of Protruding Compatible DNA Ends (C--L)

A-B    C-D-E-F-G-H-I-K-L    M-N-O-P-R    S-T-V-X-Z

Note

Single letter code:
R = G or A; 
Y = C or T; 
W = A or T; 
M = A or C; 		 K = G or T; 
S = C or G;
H = A, C or T;
V = A, C or G;		 B = C, G or T;
D = A, G or T;
N = G, A, T or C.
First restriction
enzyme		 Second restriction
enzyme		 Restriction enzymes that cleave
the newly generated recognition sequence
CfrI* (C^GGCCR) Bsp120I (G^GGCCC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI
CfrI* (Y^GGCCA) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI
CfrI* (Y^GGCCG), Eco52I (C^GGCCG) Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I
FastDigest® NotI (GC^GGCCGC), NotI (GC^GGCCGC) Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I, SatI, SsiI, TauI
CfrI* (T^GGCCR) Bsp120I (G^GGCCC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI
CfrI* (Y^GGCCA) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, MlsI
CfrI* (Y^GGCCG), Eco52I (C^GGCCG) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI
FastDigest® NotI (GC^GGCCGC), NotI (GC^GGCCGC) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, SatI, SsiI, TauI
CfrI* (C^GGCCR) Bsp120I (G^GGCCC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI
CfrI* (Y^GGCCA) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI
CfrI* (Y^GGCCG), Eco52I (C^GGCCG) Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I
FastDigest® NotI (GC^GGCCGC), NotI (GC^GGCCGC) Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I, SatI, SsiI, TauI
CfrI* (T^GGCCR) Bsp120I (G^GGCCC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI
CfrI* (Y^GGCCA) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, MlsI
CfrI* (Y^GGCCG), Eco52I (C^GGCCG) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI
FastDigest® NotI (GC^GGCCGC), NotI (GC^GGCCGC) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, SatI, SsiI, TauI
Cfr9I (C^CCGGG) AcoI* (Y^CCGGA), BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI
AcoI* (Y^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BmeT110I, BseDI, BssKI, Cfr9I, Eco88I, FastDigest® Eco88I, HpaII, FastDigest® MspI, MspI, SmaI, FastDigest® SmaI
BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGC), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGT), NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG), SgrAI* (CR^CCGGTG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI
Cfr10I* (A^CCGGY),
FastDigest® Cfr10I*
(A^CCGGY)		 AcoI* (Y^CCGGA), BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) BsaWI, HpaII, FastDigest® MspI, MspI
AcoI* (Y^CCGGG), Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI
BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT),  SgrAI* (CR^CCGGTG) BsaWI, FastDigest® BshTI, BshTI, FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI
NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG) FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI
Cfr10I* (G^CCGGY),
FastDigest® Cfr10I*
(G^CCGGY)		 AcoI* (Y^CCGGA), BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) HpaII, FastDigest® MspI, MspI
AcoI* (Y^CCGGG), Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI
BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), SgrAI* (CR^CCGGTG) FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI
NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG) Cac8I, FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI, NgoMIV, PdiI
Cfr42I (CCGC^GG) Bsh1285I* (CGGC^CG) SsiI
CpoI* (CG^GACCG) Eco47I* (G^GACC), Psp5II* (RG^GACCT) BmgT120I, Cfr13I, FastDigest® Cfr13I, Eco47I
Psp5II* (RG^GACCC), SanDI* (GG^GACCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I
CpoI* (CG^GTCCG) Eco47I* (G^GTCC), Psp5II* (RG^GTCCT) BmgT120I, Cfr13I, FastDigest® Cfr13I, Eco47I
Psp5II* (RG^GTCCC), SanDI* (GG^GTCCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I
Eco130I* (C^CATGG),
FastDigest® Eco130I*
(C^CATGG)		 AflIII* (A^CATGT), FatI (^CATG), PagI (T^CATGA), PscI (A^CATGT) CviAII, FatI, Hin1II
BtgI* (C^CATGG), FastDigest® NcoI (C^CATGG), NcoI (C^CATGG) BseDI, BtgI, CviAII, FastDigest® Eco130I, Eco130I, FatI, Hin1II, FastDigest® NcoI, NcoI
Eco130I* (C^CTAGG),
FastDigest® Eco130I*
(C^CTAGG)		 BcuI (A^CTAGT), FastDigest® BcuI (A^CTAGT), NheI (G^CTAGC), FastDigest® XbaI (T^CTAGA), XbaI (T^CTAGA) FspBI, FastDigest® FspBI
XmaJI (C^CTAGG), FastDigest® XmaJI (C^CTAGG) BseDI, FastDigest® Eco130I, Eco130I, FspBI, FastDigest® FspBI, XmaJI, FastDigest® XmaJI
Eco24I* (GAGCC^C) SduI* (GAGCC^C) CviJI, Eco24I, SduI
Eco24I* (GAGCT^C) Alw21I* (GAGCT^C), FastDigest® SacI (GAGCT^C), SacI (GAGCT^C), SduI* (GAGCT^C) AluI, Alw21I, CviJI, Ecl136II, Eco24I, FastDigest® SacI, SacI, SduI
Eco24I* (GGGCC^C) ApaI (GGGCC^C), FastDigest® ApaI (GGGCC^C), BseSI* (GGGCC^C), SduI* (GGGCC^C) ApaI, FastDigest® ApaI, BmgT120I, BseSI, Bsp120I, FastDigest® BspLI, BspLI, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, Eco24I, SduI
Eco24I* (GGGCT^C) SduI* (GGGCT^C) CviJI, Eco24I, SduI
Eco47I* (G^GACC) CpoI* (CG^GACCG), Psp5II* (RG^GACCT) BmgT120I, Cfr13I, FastDigest® Cfr13I, Eco47I
Psp5II* (RG^GACCC), SanDI* (GG^GACCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I
Eco47I* (G^GTCC) CpoI* (CG^GTCCG), Psp5II* (RG^GTCCT) BmgT120I, Cfr13I, FastDigest® Cfr13I, Eco47I
Psp5II* (RG^GTCCC), SanDI* (GG^GTCCC) BmgT120I, FastDigest® BspLI, BspLI, Cfr13I, FastDigest® Cfr13I, Eco47I
Eco52I (C^GGCCG) Bsp120I (G^GGCCC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI
CfrI* (Y^GGCCA), CfrI* (Y^GGCCA) BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI
CfrI* (Y^GGCCG), CfrI* (Y^GGCCG) Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I
FastDigest® NotI (GC^GGCCGC), NotI (GC^GGCCGC) Bsh1285I, BsuRI, FastDigest® BsuRI, CfrI, CfrI, CviJI, Eco52I, SatI, SsiI, TauI
Eco88I* (C^CCGGG),
FastDigest® Eco88I*
(C^CCGGG)		 AcoI* (Y^CCGGA), BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI
AcoI* (Y^CCGGG), Cfr9I (C^CCGGG) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BmeT110I, BseDI, BssKI, Cfr9I, Eco88I, FastDigest® Eco88I, HpaII, FastDigest® MspI, MspI, SmaI, FastDigest® SmaI
BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGC), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGT), NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG), SgrAI* (CR^CCGGTG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI
Eco88I* (C^TCGAG),
FastDigest® Eco88I*
(C^TCGAG)		 PspXI* (VC^TCGAGC), PspXI* (VC^TCGAGG), PspXI* (VC^TCGAGT), SmoI* (C^TCGAG), FastDigest® XhoI (C^TCGAG), XhoI (C^TCGAG) BmeT110I, Eco88I, FastDigest® Eco88I, SmoI, TaqI, FastDigest® TaqI, FastDigest® XhoI, XhoI
SalI (G^TCGAC), FastDigest® SalI (G^TCGAC) TaqI, FastDigest® TaqI
EcoRI (G^AATTC),
FastDigest® EcoRI
(G^AATTC)		 XapI* (R^AATTC), FastDigest® XapI* (R^AATTC) FastDigest® EcoRI, EcoRI, TasI, XapI, FastDigest® XapI
FastDigest® MunI (C^AATTG), MunI (C^AATTG), TasI (^AATT) TasI
XapI* (R^AATTT), FastDigest® XapI* (R^AATTT) TasI, XapI, FastDigest® XapI
EcoRII* (^CCAGG) SexAI* (A^CCAGGT) Bme1390I, FastDigest® Bme1390I, BssKI, EcoRII, FastDigest® MvaI, MvaI
EcoRII* (^CCTGG) SexAI* (A^CCTGGT) Bme1390I, FastDigest® Bme1390I, BssKI, EcoRII, FastDigest® MvaI, MvaI
FatI (^CATG) AflIII* (A^CATGT), BtgI* (C^CATGG), FastDigest® Eco130I* (C^CATGG), Eco130I* (C^CATGG), FastDigest® NcoI (C^CATGG), NcoI (C^CATGG), PagI (T^CATGA), PscI (A^CATGT) CviAII, FatI, Hin1II
Hin1I* (GA^CGYC) Hin6I (G^CGC), SsiI* (C^CGC) CseI
MaeII (A^CGT), Psp1406I (AA^CGTT), FastDigest® Psp1406I (AA^CGTT) MaeII, TaiI
NarI (GG^CGCC) CseI, Hin1I
Hin1I* (GG^CGYC) Hin6I (G^CGC), SsiI* (C^CGC) FastDigest® HhaI, HhaI, Hin6I
NarI (GG^CGCC) BbeI, BshNI, FastDigest® BspLI, BspLI, EheI, FastDigest® HhaI, HhaI, Hin1I, Hin6I, KasI, NarI
Hin1II (CATG) PaeI (GCATG^C), FastDigest® PaeI (GCATG^C), XceI* (RCATG^C), XceI* (RCATG^T) CviAII, FatI, Hin1II
Hin6I (G^CGC) Hin1I* (GR^CGCC), NarI (GG^CGCC), SsiI* (C^CGC) FastDigest® HhaI, HhaI, Hin6I
HpaII (C^CGG) BmeT110I* (CY^CGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI
Hin1I* (GR^CGCC), Hin6I (G^CGC), NarI (GG^CGCC), SsiI* (C^CGC) SsiI
FastDigest® MspI (C^CGG), MspI (C^CGG), SsiI* (G^CGG) HpaII, FastDigest® MspI, MspI
KasI (G^GCGCC) BshNI* (G^GCGCC) BbeI, BshNI, FastDigest® BspLI, BspLI, EheI, FastDigest® HhaI, HhaI, Hin1I, Hin6I, KasI, NarI
Kpn2I (T^CCGGA),
FastDigest® Kpn2I
(T^CCGGA)		 AcoI* (Y^CCGGA), BsaWI* (W^CCGGA) AcoI, BsaWI, HpaII, Hpy188III, FastDigest® Kpn2I, Kpn2I, FastDigest® MspI, MspI
AcoI* (Y^CCGGG), Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI
BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGT), SgrAI* (CR^CCGGTG) BsaWI, HpaII, FastDigest® MspI, MspI
FastDigest® Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGC), NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG) HpaII, FastDigest® MspI, MspI

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Updated sausio 31, 2008 08:52