Newly Generated Recognition Sequences Resulting from:
- Ligation of Protruding Compatible DNA Ends (see below)
- Fill-in of a 5'-overhang and Self-ligation
- Removal of a 3'-overhang and Self-ligation
Newly Generated Recognition Sequences Resulting from the Ligation of Protruding Compatible DNA Ends (A--B)
A-B C-D-E-F-G-H-I-K-L M-N-O-P-R S-T-V-X-Z
Note
- Restriction enzymes that have degenerate recognition sequences (i.e., recognize more than one sequence) are indicated by an asterisk (*). Be aware that these restriction enzymes will cleave sequences in addition to the one listed.
- Enzymes produced by Fermentas are shown in green.
Single letter code: R = G or A;
Y = C or T;
W = A or T;
M = A or C;K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;B = C, G or T;
D = A, G or T;
N = G, A, T or C.
First restriction
enzymeSecond restriction
enzymeRestriction enzymes that cleave
the newly generated recognition sequenceAatII (GACGT^C) TaiI (ACGT) MaeII, TaiI Acc65I (G^GTACC) BshNI* (G^GTACC) Acc65I, BshNI, FastDigest® BspLI, BspLI, Csp6I, KpnI, FastDigest® KpnI, FastDigest® RsaI, RsaI Bsp1407I (T^GTACA), FastDigest® Bsp1407I (T^GTACA), Pfl23II (C^GTACG), TatI* (W^GTACA), TatI* (W^GTACT) Csp6I, FastDigest® RsaI, RsaI AcoI* (C^CCGGR) BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGC), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGT), NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG), SgrAI* (CR^CCGGTG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BmeT110I, BseDI, BssKI, Cfr9I, Eco88I, FastDigest® Eco88I, HpaII, FastDigest® MspI, MspI, SmaI, FastDigest® SmaI AcoI* (T^CCGGR) BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) AcoI, BsaWI, HpaII, Hpy188III, FastDigest® Kpn2I, Kpn2I, FastDigest® MspI, MspI BsaWI* (W^CCGGT), FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGT), SgrAI* (CR^CCGGTG) BsaWI, HpaII, FastDigest® MspI, MspI FastDigest® Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGC), NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG) HpaII, FastDigest® MspI, MspI Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI AflIII* (A^CATGT) BtgI* (C^CATGG), FastDigest® Eco130I* (C^CATGG), Eco130I* (C^CATGG), FatI (^CATG), FastDigest® NcoI (C^CATGG), NcoI (C^CATGG), PagI (T^CATGA) CviAII, FatI, Hin1II PscI (A^CATGT) AflIII, CviAII, FatI, Hin1II, PscI, XceI AflIII* (A^CGCGT) BtgI* (C^CGCGG) Bsh1236I MluI (A^CGCGT), FastDigest® MluI (A^CGCGT) AflIII, Bsh1236I, MluI, FastDigest® MluI PauI (G^CGCGC), SgsI (GG^CGCGCC) Bsh1236I, FastDigest® HhaI, HhaI, Hin6I AflIII* (A^CGTGT) BtgI* (C^CGTGG) MaeII, TaiI Alw21I* (GAGCA^C) SduI* (GAGCA^C) Alw21I, SduI Alw21I* (GAGCT^C) Eco24I* (GAGCT^C), FastDigest® SacI (GAGCT^C), SacI (GAGCT^C), SduI* (GAGCT^C) AluI, Alw21I, CviJI, Ecl136II, Eco24I, FastDigest® SacI, SacI, SduI Alw21I* (GTGCA^C) BseSI* (GTGCA^C), SduI* (GTGCA^C) Alw21I, Alw44I, BseSI, FastDigest® Hpy8I, Hpy8I, HpyCH4V, SduI FastDigest® Mph1103I (ATGCA^T), Mph1103I (ATGCA^T) HpyCH4V PstI (CTGCA^G), FastDigest® PstI (CTGCA^G), FastDigest® SdaI (CCTGCA^GG), SdaI (CCTGCA^GG) BsgI, HpyCH4V Alw21I* (GTGCT^C) SduI* (GTGCT^C) Alw21I, SduI Alw44I (G^TGCAC) BfmI* (C^TGCAG) BsgI, HpyCH4V ApaI (GGGCC^C),
FastDigest® ApaI
(GGGCC^C)BseSI* (GGGCC^C), Eco24I* (GGGCC^C), SduI* (GGGCC^C) ApaI, FastDigest® ApaI, BmgT120I, BseSI, Bsp120I, FastDigest® BspLI, BspLI, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, Eco24I, SduI BamHI (G^GATCC),
FastDigest® BamHI
(G^GATCC)PsuI* (R^GATCC) FastDigest® BamHI, BamHI, Bsp143I, FastDigest® BspLI, BspLI, BspPI, DpnI, MboI, PsuI BclI (T^GATCA), Bsp143I (^GATC), MboI (^GATC) Bsp143I, BspPI, DpnI, MboI BglII (A^GATCT), FastDigest® BglII (A^GATCT), PsuI* (R^GATCT) Bsp143I, BspPI, DpnI, MboI, PsuI BclI (T^GATCA) FastDigest® BamHI (G^GATCC), BamHI (G^GATCC), BglII (A^GATCT), FastDigest® BglII (A^GATCT), Bsp143I (^GATC), MboI (^GATC), PsuI* (R^GATCC), PsuI* (R^GATCT) Bsp143I, DpnI, MboI BcuI (A^CTAGT),
FastDigest® BcuI
(A^CTAGT)FastDigest® Eco130I* (C^CTAGG), Eco130I* (C^CTAGG), NheI (G^CTAGC), FastDigest® XbaI (T^CTAGA), XbaI (T^CTAGA), XmaJI (C^CTAGG), FastDigest® XmaJI (C^CTAGG) FspBI, FastDigest® FspBI BfmI* (C^TGCAG) Alw44I (G^TGCAC) HpyCH4V BglII (A^GATCT),
FastDigest® BglII
(A^GATCT)FastDigest® BamHI (G^GATCC), BamHI (G^GATCC), PsuI* (R^GATCC) Bsp143I, DpnI, MboI, PsuI BclI (T^GATCA), Bsp143I (^GATC), MboI (^GATC) Bsp143I, DpnI, MboI PsuI* (R^GATCT) BglII, FastDigest® BglII, Bsp143I, DpnI, MboI, PsuI BmeT110I* (CC^CGRG) Hin1I* (GR^CGCC), Hin6I (G^CGC), NarI (GG^CGCC), SsiI* (C^CGC) SmuI, SsiI HpaII (C^CGG), FastDigest® MspI (C^CGG), MspI (C^CGG), SsiI* (G^CGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI BmeT110I* (CT^CGRG) Bsp119I (TT^CGAA), FastDigest® Bsp119I (TT^CGAA), FastDigest® Bsu15I (AT^CGAT), Bsu15I (AT^CGAT), TaqI (T^CGA), FastDigest® TaqI (T^CGA), FastDigest® XmiI* (GT^CGAC), XmiI* (GT^CGAC) TaqI, FastDigest® TaqI BsaWI* (A^CCGGW) AcoI* (Y^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) BsaWI, HpaII, FastDigest® MspI, MspI AcoI* (Y^CCGGG), Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGT), SgrAI* (CR^CCGGTG) BsaWI, FastDigest® BshTI, BshTI, FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI FastDigest® Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGC), NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG) FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI BsaWI* (T^CCGGW) AcoI* (Y^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) AcoI, BsaWI, HpaII, Hpy188III, FastDigest® Kpn2I, Kpn2I, FastDigest® MspI, MspI AcoI* (Y^CCGGG), Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI FastDigest® BshTI (A^CCGGT), BshTI (A^CCGGT), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGT), SgrAI* (CR^CCGGTG) BsaWI, HpaII, FastDigest® MspI, MspI FastDigest® Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGC), NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG) HpaII, FastDigest® MspI, MspI BseSI* (GGGCA^C) SduI* (GGGCA^C) BseSI, SduI BseSI* (GGGCC^C) ApaI (GGGCC^C), FastDigest® ApaI (GGGCC^C), Eco24I* (GGGCC^C), SduI* (GGGCC^C) ApaI, FastDigest® ApaI, BmgT120I, BseSI, Bsp120I, FastDigest® BspLI, BspLI, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, Eco24I, SduI BseSI* (GTGCA^C) Alw21I* (GTGCA^C), SduI* (GTGCA^C) Alw21I, Alw44I, BseSI, FastDigest® Hpy8I, Hpy8I, HpyCH4V, SduI FastDigest® Mph1103I (ATGCA^T), Mph1103I (ATGCA^T) HpyCH4V PstI (CTGCA^G), FastDigest® PstI (CTGCA^G), FastDigest® SdaI (CCTGCA^GG), SdaI (CCTGCA^GG) BsgI, HpyCH4V BseSI* (GTGCC^C) SduI* (GTGCC^C) BseSI, SduI Bsh1285I* (CGAT^CG) PacI (TTAAT^TAA) Tru1I PvuI (CGAT^CG), FastDigest® PvuI (CGAT^CG), SgfI (GCGAT^CGC) Bsh1285I, Bsp143I, DpnI, MboI, PvuI, FastDigest® PvuI BshNI* (G^GCGCC) KasI (G^GCGCC) BbeI, BshNI, FastDigest® BspLI, BspLI, EheI, FastDigest® HhaI, HhaI, Hin1I, Hin6I, KasI, NarI BshNI* (G^GTACC) Acc65I (G^GTACC) Acc65I, BshNI, FastDigest® BspLI, BspLI, Csp6I, KpnI, FastDigest® KpnI, FastDigest® RsaI, RsaI Bsp1407I (T^GTACA), FastDigest® Bsp1407I (T^GTACA), Pfl23II (C^GTACG), TatI* (W^GTACA), TatI* (W^GTACT) Csp6I, FastDigest® RsaI, RsaI BshTI (A^CCGGT),
FastDigest® BshTI
(A^CCGGT)AcoI* (Y^CCGGA), BsaWI* (W^CCGGA), FastDigest® Kpn2I (T^CCGGA), Kpn2I (T^CCGGA) BsaWI, HpaII, FastDigest® MspI, MspI AcoI* (Y^CCGGG), Cfr9I (C^CCGGG), Eco88I* (C^CCGGG), FastDigest® Eco88I* (C^CCGGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI BsaWI* (W^CCGGT), FastDigest® Cfr10I* (R^CCGGT), Cfr10I* (R^CCGGT), SgrAI* (CR^CCGGTG) BsaWI, FastDigest® BshTI, BshTI, FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI FastDigest® Cfr10I* (R^CCGGC), Cfr10I* (R^CCGGC), NgoMIV (G^CCGGC), SgrAI* (CR^CCGGCG) FastDigest® Cfr10I, Cfr10I, HpaII, FastDigest® MspI, MspI Bsp119I (TT^CGAA),
FastDigest® Bsp119I
(TT^CGAA)BmeT110I* (CY^CGAG), FastDigest® Bsu15I (AT^CGAT), Bsu15I (AT^CGAT), TaqI (T^CGA), FastDigest® TaqI (T^CGA), FastDigest® XmiI* (GT^CGAC), XmiI* (GT^CGAC) TaqI, FastDigest® TaqI Bsp120I (G^GGCCC) CfrI* (Y^GGCCA), CfrI* (Y^GGCCG), CfrI* (Y^GGCCA), CfrI* (Y^GGCCG), Eco52I (C^GGCCG) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI FastDigest® NotI (GC^GGCCGC), NotI (GC^GGCCGC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI, SatI, SsiI, TauI Bsp1407I (T^GTACA),
FastDigest® Bsp1407I
(T^GTACA)Acc65I (G^GTACC), BshNI* (G^GTACC), Pfl23II (C^GTACG) Csp6I, FastDigest® RsaI, RsaI TatI* (W^GTACA) Bsp1407I, FastDigest® Bsp1407I, Csp6I, FastDigest® RsaI, RsaI, TatI TatI* (W^GTACT) Csp6I, FastDigest® RsaI, RsaI, TatI Bsp143I (^GATC) FastDigest® BamHI (G^GATCC), BamHI (G^GATCC), BclI (T^GATCA), BglII (A^GATCT), FastDigest® BglII (A^GATCT), MboI (^GATC), PsuI* (R^GATCC), PsuI* (R^GATCT) Bsp143I, DpnI, MboI BspTI (C^TTAAG) SmoI* (C^TTAAG) BspTI, SmoI, Tru1I Bsu15I (AT^CGAT),
FastDigest® Bsu15I
(AT^CGAT)BmeT110I* (CY^CGAG), Bsp119I (TT^CGAA), FastDigest® Bsp119I (TT^CGAA), TaqI (T^CGA), FastDigest® TaqI (T^CGA), FastDigest® XmiI* (GT^CGAC), XmiI* (GT^CGAC) TaqI, FastDigest® TaqI BtgI* (C^CACGG) AflIII* (A^CACGT) MaeII, TaiI BtgI* (C^CATGG) AflIII* (A^CATGT), FatI (^CATG), PagI (T^CATGA), PscI (A^CATGT) CviAII, FatI, Hin1II FastDigest® Eco130I* (C^CATGG), Eco130I* (C^CATGG), FastDigest® NcoI (C^CATGG), NcoI (C^CATGG) BseDI, BtgI, CviAII, FastDigest® Eco130I, Eco130I, FatI, Hin1II, FastDigest® NcoI, NcoI BtgI* (C^CGCGG) AflIII* (A^CGCGT), MluI (A^CGCGT), FastDigest® MluI (A^CGCGT) Bsh1236I, SsiI PauI (G^CGCGC), SgsI (GG^CGCGCC) Bsh1236I, FastDigest® HhaI, HhaI, Hin6I, SsiI
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Updated sausio 31, 2008 08:52
