Newly Generated Recognition Sequences Resulting from:
Ligation of Blunt DNA Ends (see below)
- Ligation of Protruding Compatible DNA Ends
- Fill-in of a 5'-overhang and Self-ligation
- Removal of a 3'-overhang and Self-ligation
Newly Generated Recognition Sequences Resulting from the Ligation of Blunt DNA Ends (M--Z)
A-B C-D-E F-G-H-I-K-L M-N-O-P-R-S-T-V-X-Z
Note
- Restriction enzymes that have degenerate recognition sequences (i.e., recognize more than one sequence) are indicated by an asterisk (*). Be aware that these restriction enzymes will cleave sequences in addition to the one listed.
- Enzymes produced by Fermentas are shown in green.
Single letter code: R = G or A;
Y = C or T;
W = A or T;
M = A or C;K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;B = C, G or T;
D = A, G or T;
N = G, A, T or C.
First restriction
enzymeSecond restriction
enzymeRestriction enzymes that cleave
the newly generated recognition sequenceMbiI* (CCG^CTC) AjiI* (CAC^GTC), AjiI* (GAC^GTG), FastDigest® Eco105I (TAC^GTA), Eco105I (TAC^GTA), Eco72I (CAC^GTG), FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA), Ppu21I* (YAC^GTA), Ppu21I* (YAC^GTG), ZraI (GAC^GTC) HpaII, FastDigest® MspI, MspI AluI (AG^CT), BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), CviJI* (RG^CT), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), HpyCH4V (TG^CA), MlsI (TGG^CCA) SsiI Bsh1236I (CG^CG), Bsp68I (TCG^CGA) Bsh1236I, SsiI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI Ecl136II (GAG^CTC) MbiI, SsiI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, DpnI, MboI Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT) CviJI, HpaII, FastDigest® MspI, MspI EheI (GGC^GCC) BsuRI, FastDigest® BsuRI, CviJI, HpaII, FastDigest® MspI, MspI MspA1I* (CMG^CGG) BseDI, Bsh1236I, BtgI, Cfr42I, MspA1I, SsiI MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) MspA1I, SsiI PdiI (GCC^GGC) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI SmaI (CCC^GGG), FastDigest® SmaI (CCC^GGG), SrfI (GCCC^GGGC) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BseDI, BssKI, HpaII, FastDigest® MspI, MspI MbiI* (GAG^CGG) AluI (AG^CT), CviJI* (RG^CT), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) AluI, CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MlsI (TGG^CCA) CviJI DpnI (GA^TC) FastDigest® HinfI, HinfI, PleI, SchI Ecl136II (GAG^CTC) AluI, Alw21I, CviJI, Ecl136II, Eco24I, FastDigest® SacI, SacI, SduI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, DpnI, MboI EheI (GGC^GCC) BsuRI, FastDigest® BsuRI, CviJI MlsI (TGG^CCA) AluI (AG^CT), CviJI* (RG^CT), Ecl136II (GAG^CTC), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MbiI* (CCG^CTC), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT) BsuRI, FastDigest® BsuRI, CviJI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, BspPI, DpnI, MboI EheI (GGC^GCC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI FspAI* (RTGC^GCAC) BseSI, SduI FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC) FaqI MspA1I* (CAG^CKG) AluI (AG^CT), CviJI* (RG^CT), Ecl136II (GAG^CTC), MbiI* (CCG^CTC) AluI, CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MlsI (TGG^CCA) CviJI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, DpnI, MboI EheI (GGC^GCC) BsuRI, FastDigest® BsuRI, CviJI MbiI* (GAG^CGG) MspA1I PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) AluI, CviJI, MspA1I, PvuII, FastDigest® PvuII MspA1I* (CCG^CKG) AjiI* (CAC^GTC), AjiI* (GAC^GTG), FastDigest® Eco105I (TAC^GTA), Eco105I (TAC^GTA), Eco72I (CAC^GTG), FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA), Ppu21I* (YAC^GTA), Ppu21I* (YAC^GTG), ZraI (GAC^GTC) HpaII, FastDigest® MspI, MspI AluI (AG^CT), BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), CviJI* (RG^CT), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), HpyCH4V (TG^CA), MlsI (TGG^CCA) SsiI Bsh1236I (CG^CG), Bsp68I (TCG^CGA) Bsh1236I, SsiI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MbiI, SsiI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, DpnI, MboI Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT) CviJI, HpaII, FastDigest® MspI, MspI EheI (GGC^GCC) BsuRI, FastDigest® BsuRI, CviJI, HpaII, FastDigest® MspI, MspI MbiI* (GAG^CGG) BseDI, Bsh1236I, BtgI, Cfr42I, MspA1I, SsiI PdiI (GCC^GGC) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) MspA1I, SsiI SmaI (CCC^GGG), FastDigest® SmaI (CCC^GGG), SrfI (GCCC^GGGC) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BseDI, BssKI, HpaII, FastDigest® MspI, MspI MssI (GTTT^AAAC) Bsp68I (TCG^CGA) TaqI, FastDigest® TaqI DraI (TTT^AAA), FastDigest® DraI (TTT^AAA), SmiI (ATTT^AAAT), FastDigest® SmiI (ATTT^AAAT) DraI, FastDigest® DraI, Tru1I FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) HpyCH4V FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC), PsiI (TTA^TAA) Tru1I FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT) FastDigest® Hpy8I, Hpy8I NsbI (TGC^GCA),
FastDigest® NsbI
(TGC^GCA)DraI (TTT^AAA), FastDigest® DraI (TTT^AAA), FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC), MssI (GTTT^AAAC), FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT), SmiI (ATTT^AAAT), FastDigest® SmiI (ATTT^AAAT), SspI (AAT^ATT), FastDigest® SspI (AAT^ATT) HpyCH4V Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MnlI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) HpyCH4V, LweI Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), EheI (GGC^GCC) FastDigest® HhaI, HhaI, Hin6I FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT) FastDigest® HhaI, HhaI, Hin6I, FastDigest® NsbI, NsbI MbiI* (GAG^CGG), MspA1I* (CMG^CGG) HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) SatI, TauI SrfI (GCCC^GGGC) Cac8I PdiI (GCC^GGC) DpnI (GA^TC), Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MnlI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) BccI Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), EheI (GGC^GCC), FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) SatI, SsiI, TauI MbiI* (GAG^CGG), MspA1I* (CMG^CGG), SmaI (CCC^GGG), FastDigest® SmaI (CCC^GGG), SrfI (GCCC^GGGC) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI Ppu21I* (CAC^GTR) AjiI* (CAC^GTC), ZraI (GAC^GTC) AjiI, MaeII, TaiI AjiI* (GAC^GTG), Eco72I (CAC^GTG) Eco72I, MaeII, Ppu21I, TaiI Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MnlI FastDigest® Eco105I (TAC^GTA), Eco105I (TAC^GTA) MaeII, Ppu21I, TaiI MbiI* (GAG^CGG), MspA1I* (CMG^CGG) HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) BceAI Ppu21I* (TAC^GTR) AjiI* (CAC^GTC), ZraI (GAC^GTC) MaeII, TaiI AjiI* (GAC^GTG), Eco72I (CAC^GTG) MaeII, Ppu21I, TaiI Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MnlI FastDigest® Eco105I (TAC^GTA), Eco105I (TAC^GTA) FastDigest® Eco105I, Eco105I, MaeII, Ppu21I, TaiI MbiI* (GAG^CGG), MspA1I* (CMG^CGG) HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) BceAI PsiI (TTA^TAA) DraI (TTT^AAA), FastDigest® DraI (TTT^AAA), FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC), FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC), MssI (GTTT^AAAC), FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT), SmiI (ATTT^AAAT), FastDigest® SmiI (ATTT^AAAT) Tru1I Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT) AluI, CviJI EheI (GGC^GCC) CviJI SspI (AAT^ATT), FastDigest® SspI (AAT^ATT) TasI, Tru1I PvuII (CAG^CTG),
FastDigest® PvuII
(CAG^CTG)AluI (AG^CT), CviJI* (RG^CT), Ecl136II (GAG^CTC), MbiI* (CCG^CTC) AluI, CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MlsI (TGG^CCA) CviJI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, DpnI, MboI EheI (GGC^GCC) BsuRI, FastDigest® BsuRI, CviJI MbiI* (GAG^CGG), MspA1I* (CMG^CGG) MspA1I MspA1I* (CMG^CTG) AluI, CviJI, MspA1I, PvuII, FastDigest® PvuII RsaI (GT^AC),
FastDigest® RsaI
(GT^AC)Bsp68I (TCG^CGA) TaqI, FastDigest® TaqI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC), FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC) MaeIII Ecl136II (GAG^CTC), MbiI* (CCG^CTC) FastDigest® Alw26I, Alw26I FspAI* (RTGC^GCAC) Alw21I, Alw44I, BseSI, FastDigest® Hpy8I, Hpy8I, HpyCH4V, SduI FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) HpyCH4V FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC) MaeIII, NmuCI, FastDigest® NmuCI MssI (GTTT^AAAC) FastDigest® Hpy8I, Hpy8I PsiI (TTA^TAA) Tru1I ScaI (AGT^ACT) Csp6I, FastDigest® RsaI, RsaI ScaI (AGT^ACT) Bsp68I (TCG^CGA) TaqI, FastDigest® TaqI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC), FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC) MaeIII Ecl136II (GAG^CTC), MbiI* (CCG^CTC) FastDigest® Alw26I, Alw26I FspAI* (RTGC^GCAC) Alw21I, Alw44I, BseSI, FastDigest® Hpy8I, Hpy8I, HpyCH4V, SduI FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) HpyCH4V FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC) MaeIII, NmuCI, FastDigest® NmuCI MssI (GTTT^AAAC) FastDigest® Hpy8I, Hpy8I PsiI (TTA^TAA) Tru1I FastDigest® RsaI (GT^AC), RsaI (GT^AC) Csp6I, FastDigest® RsaI, RsaI SmaI (CCC^GGG),
FastDigest® SmaI
(CCC^GGG)DpnI (GA^TC), Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MnlI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) BccI Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), EheI (GGC^GCC), FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) SmuI, SsiI MbiI* (GAG^CGG), MspA1I* (CMG^CGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BseDI, BssKI, HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI SrfI (GCCC^GGGC) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BmeT110I, BseDI, BssKI, Cfr9I, Eco88I, FastDigest® Eco88I, HpaII, FastDigest® MspI, MspI, SmaI, FastDigest® SmaI SmiI (ATTT^AAAT),
FastDigest® SmiI
(ATTT^AAAT)Bsp68I (TCG^CGA) TaqI, FastDigest® TaqI DraI (TTT^AAA), FastDigest® DraI (TTT^AAA), MssI (GTTT^AAAC) DraI, FastDigest® DraI, Tru1I FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) HpyCH4V FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC), PsiI (TTA^TAA) Tru1I SrfI (GCCC^GGGC) DpnI (GA^TC), Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MnlI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) BccI Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), EheI (GGC^GCC), FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) Cac8I, SmuI, SsiI MbiI* (GAG^CGG), MspA1I* (CMG^CGG) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BseDI, BssKI, HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BssKI, HpaII, FastDigest® MspI, MspI SmaI (CCC^GGG), FastDigest® SmaI (CCC^GGG) AcoI, FastDigest® BcnI, BcnI, Bme1390I, FastDigest® Bme1390I, BmeT110I, BseDI, BssKI, Cfr9I, Eco88I, FastDigest® Eco88I, HpaII, FastDigest® MspI, MspI, SmaI, FastDigest® SmaI SspI (AAT^ATT),
FastDigest® SspI
(AAT^ATT)Bsp68I (TCG^CGA) TaqI, FastDigest® TaqI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC), DpnI (GA^TC) TasI FspAI* (RTGC^GCAC), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) HpyCH4V FspAI* (RTGC^GCAT) HpyCH4V, FastDigest® Mph1103I, Mph1103I PsiI (TTA^TAA) TasI, Tru1I ZraI (GAC^GTC) AjiI* (CAC^GTC) AatII, Hin1I, MaeII, TaiI, ZraI AjiI* (GAC^GTG), FastDigest® Eco105I (TAC^GTA), Eco105I (TAC^GTA), Eco72I (CAC^GTG), Ppu21I* (YAC^GTA), Ppu21I* (YAC^GTG) MaeII, TaiI Bsp68I (TCG^CGA) TaqII DpnI (GA^TC) FastDigest® HinfI, HinfI Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MnlI Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) CseI EheI (GGC^GCC) CseI, Hin1I MbiI* (GAG^CGG), MspA1I* (CMG^CGG) HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) BceAI
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Updated sausio 31, 2008 08:52
