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Newly Generated Recognition Sequences Resulting from:

• Ligation of Blunt DNA Ends (see below)

• Ligation of Protruding Compatible DNA Ends
• Fill-in of a 5'-overhang and Self-ligation
• Removal of a 3'-overhang and Self-ligation

Newly Generated Recognition Sequences Resulting from the Ligation of Blunt DNA Ends (F--L)

A-B    C-D-E    F-G-H-I-K-L    M-N-O-P-R-S-T-V-X-Z

Note

• Restriction enzymes that have degenerate recognition sequences (i.e., recognize more than one sequence) are indicated by an asterisk (*). Be aware that these restriction enzymes will cleave sequences in addition to the one listed.
• Enzymes produced by Fermentas are shown in green.

Single letter code:
R = G or A;  Y = C or T;  W = A or T;  M = A or C;	K = G or T;  S = C or G; H = A, C or T; V = A, C or G;	B = C, G or T; D = A, G or T; N = G, A, T or C.

First restriction enzyme	Second restriction enzyme	Restriction enzymes that cleave the newly generated recognition sequence
FspAI* (ATGC^GCAY)	DraI (TTT^AAA), FastDigest® DraI (TTT^AAA), FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC), MssI (GTTT^AAAC), FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT), SmiI (ATTT^AAAT), FastDigest® SmiI (ATTT^AAAT)	HpyCH4V
	Ecl136II (GAG^CTC), MbiI* (CCG^CTC)	MnlI
	FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC)	HpyCH4V, LweI, FastDigest® Mph1103I, Mph1103I
	Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), EheI (GGC^GCC)	FastDigest® HhaI, HhaI, Hin6I
	MbiI* (GAG^CGG), MspA1I* (CMG^CGG)	HpaII, FastDigest® MspI, MspI
	FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA)	FastDigest® HhaI, HhaI, Hin6I, FastDigest® NsbI, NsbI
	PdiI (GCC^GGC)	SatI, TauI
	SrfI (GCCC^GGGC)	Cac8I
	SspI (AAT^ATT), FastDigest® SspI (AAT^ATT)	HpyCH4V, FastDigest® Mph1103I, Mph1103I
FspAI* (GTGC^GCAY)	BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), MlsI (TGG^CCA)	BseSI, SduI
	DpnI (GA^TC)	Alw21I, SduI
	DraI (TTT^AAA), FastDigest® DraI (TTT^AAA), FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC), MssI (GTTT^AAAC), SmiI (ATTT^AAAT), FastDigest® SmiI (ATTT^AAAT), SspI (AAT^ATT), FastDigest® SspI (AAT^ATT)	HpyCH4V
	Ecl136II (GAG^CTC), MbiI* (CCG^CTC)	MnlI
	FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC)	HpyCH4V, LweI
	Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), EheI (GGC^GCC)	FastDigest® HhaI, HhaI, Hin6I
	MbiI* (GAG^CGG), MspA1I* (CMG^CGG)	HpaII, FastDigest® MspI, MspI
	FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA)	FastDigest® HhaI, HhaI, Hin6I, FastDigest® NsbI, NsbI
	PdiI (GCC^GGC)	SatI, TauI
	FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT)	Alw21I, Alw44I, BseSI, FastDigest® Hpy8I, Hpy8I, HpyCH4V, SduI
	SrfI (GCCC^GGGC)	Cac8I
HincII* (GTC^RAC), FastDigest® HincII* (GTC^RAC)	Bsp68I (TCG^CGA)	Hpy188I
	Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC)	FastDigest® Hpy8I, Hpy8I, FastDigest® XmiI, XmiI
	DpnI (GA^TC)	FastDigest® Alw26I, Alw26I
	Ecl136II (GAG^CTC), MbiI* (CCG^CTC)	MnlI
	KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC)	FastDigest® HincII, HincII, FastDigest® Hpy8I, Hpy8I
	MbiI* (GAG^CGG), MspA1I* (CMG^CGG)	HpaII, FastDigest® MspI, MspI
	FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT)	MaeIII, NmuCI, FastDigest® NmuCI
HincII* (GTT^RAC), FastDigest® HincII* (GTT^RAC)	Bsp68I (TCG^CGA)	TaqI, FastDigest® TaqI
	Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC)	FastDigest® Hpy8I, Hpy8I
	DraI (TTT^AAA), FastDigest® DraI (TTT^AAA), MssI (GTTT^AAAC), PsiI (TTA^TAA), SmiI (ATTT^AAAT), FastDigest® SmiI (ATTT^AAAT)	Tru1I
	FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA)	HpyCH4V
	KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC)	FastDigest® HincII, HincII, FastDigest® Hpy8I, Hpy8I, KspAI, FastDigest® KspAI, Tru1I
	FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT)	MaeIII
HpyCH4V (TG^CA)	Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC)	Csp6I, FastDigest® RsaI, RsaI
	FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC)	Bsp143I, DpnI, MboI
	Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT)	CviJI
	EheI (GGC^GCC)	BsuRI, FastDigest® BsuRI, CviJI
KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC)	Bsp68I (TCG^CGA)	TaqI, FastDigest® TaqI
	Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC)	FastDigest® Hpy8I, Hpy8I
	DraI (TTT^AAA), FastDigest® DraI (TTT^AAA), MssI (GTTT^AAAC), PsiI (TTA^TAA), SmiI (ATTT^AAAT), FastDigest® SmiI (ATTT^AAAT)	Tru1I
	FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA)	HpyCH4V
	FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC)	FastDigest® HincII, HincII, FastDigest® Hpy8I, Hpy8I, KspAI, FastDigest® KspAI, Tru1I
	FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC)	FastDigest® HincII, HincII, FastDigest® Hpy8I, Hpy8I
	FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT)	MaeIII

catalog@fermentas.com

Updated sausio 31, 2008 08:52