Newly Generated Recognition Sequences Resulting from:
Ligation of Blunt DNA Ends (see below)
- Ligation of Protruding Compatible DNA Ends
- Fill-in of a 5'-overhang and Self-ligation
- Removal of a 3'-overhang and Self-ligation
Newly Generated Recognition Sequences Resulting from the Ligation of Blunt DNA Ends (C--E)
A-B C-D-E F-G-H-I-K-L M-N-O-P-R-S-T-V-X-Z
Note
- Restriction enzymes that have degenerate recognition sequences (i.e., recognize more than one sequence) are indicated by an asterisk (*). Be aware that these restriction enzymes will cleave sequences in addition to the one listed.
- Enzymes produced by Fermentas are shown in green.
Single letter code: R = G or A;
Y = C or T;
W = A or T;
M = A or C;K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;B = C, G or T;
D = A, G or T;
N = G, A, T or C.
First restriction
enzymeSecond restriction
enzymeRestriction enzymes that cleave
the newly generated recognition sequenceCviJI* (AG^CY) AluI (AG^CT), Ecl136II (GAG^CTC), MbiI* (CCG^CTC), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) AluI, CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MlsI (TGG^CCA) CviJI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, DpnI, MboI EheI (GGC^GCC) BsuRI, FastDigest® BsuRI, CviJI CviJI* (GG^CY) AluI (AG^CT), Ecl136II (GAG^CTC), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MbiI* (CCG^CTC), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), MlsI (TGG^CCA) BsuRI, FastDigest® BsuRI, CviJI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, BspPI, DpnI, MboI EheI (GGC^GCC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI FspAI* (RTGC^GCAC) BseSI, SduI FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC) FaqI DpnI (GA^TC) AjiI* (CAC^GTC), ZraI (GAC^GTC) FastDigest® HinfI, HinfI, PleI, SchI Ecl136II (GAG^CTC), MbiI* (CCG^CTC) FastDigest® HinfI, HinfI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) FastDigest® HinfI, HinfI, PfeI Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT) AluI, CviJI EheI (GGC^GCC) CviJI FspAI* (RTGC^GCAC) Alw21I, SduI SspI (AAT^ATT), FastDigest® SspI (AAT^ATT) TasI DraI (TTT^AAA),
FastDigest® DraI
(TTT^AAA)Bsp68I (TCG^CGA) TaqI, FastDigest® TaqI MssI (GTTT^AAAC), SmiI (ATTT^AAAT), FastDigest® SmiI (ATTT^AAAT) DraI, FastDigest® DraI, Tru1I FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) HpyCH4V FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC), PsiI (TTA^TAA) Tru1I Ecl136II (GAG^CTC) AluI (AG^CT), CviJI* (RG^CT), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) AluI, CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MlsI (TGG^CCA) CviJI DpnI (GA^TC) FastDigest® HinfI, HinfI, PleI, SchI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, DpnI, MboI EheI (GGC^GCC) BsuRI, FastDigest® BsuRI, CviJI MbiI* (CCG^CTC) AluI, Alw21I, CviJI, Ecl136II, Eco24I, FastDigest® SacI, SacI, SduI Eco105I (TAC^GTA),
FastDigest® Eco105I
(TAC^GTA)AjiI* (CAC^GTC), ZraI (GAC^GTC) MaeII, TaiI AjiI* (GAC^GTG), Eco72I (CAC^GTG), Ppu21I* (YAC^GTG) MaeII, Ppu21I, TaiI Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MnlI Ppu21I* (YAC^GTA) FastDigest® Eco105I, Eco105I, MaeII, Ppu21I, TaiI MbiI* (GAG^CGG), MspA1I* (CMG^CGG) HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) BceAI Eco147I (AGG^CCT),
FastDigest® Eco147I
(AGG^CCT)AluI (AG^CT), CviJI* (RG^CT), Ecl136II (GAG^CTC), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MbiI* (CCG^CTC), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC) Csp6I, FastDigest® RsaI, RsaI BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), MlsI (TGG^CCA) BsuRI, FastDigest® BsuRI, CviJI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) Bsp143I, BspPI, DpnI, MboI EheI (GGC^GCC) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI FspAI* (RTGC^GCAC) BseSI, SduI FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC) FaqI Eco32I (GAT^ATC),
FastDigest® Eco32I
(GAT^ATC)AluI (AG^CT), Bsh1236I (CG^CG), BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), CviJI* (RG^CT), Ecl136II (GAG^CTC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), HpyCH4V (TG^CA), MbiI* (CCG^CTC), MbiI* (GAG^CGG), MlsI (TGG^CCA), MspA1I* (CMG^CGG), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) Bsp143I, DpnI, MboI Bsp68I (TCG^CGA) Bsp143I, DpnI, MboI, TaqI, FastDigest® TaqI DpnI (GA^TC) FastDigest® HinfI, HinfI, PfeI FspAI* (RTGC^GCAC), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) HpyCH4V FspAI* (RTGC^GCAT) HpyCH4V, FastDigest® Mph1103I, Mph1103I PsiI (TTA^TAA) Tru1I Eco47III (AGC^GCT),
FastDigest® Eco47III
(AGC^GCT)AluI (AG^CT), Bsh1236I (CG^CG), Bsp68I (TCG^CGA), BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), CviJI* (RG^CT), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), HpyCH4V (TG^CA), MlsI (TGG^CCA), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC), DpnI (GA^TC), PsiI (TTA^TAA) AluI, CviJI Ecl136II (GAG^CTC), MbiI* (CCG^CTC) CviJI, MnlI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) LweI EheI (GGC^GCC) FastDigest® HhaI, HhaI, Hin6I FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) FastDigest® HhaI, HhaI, Hin6I MbiI* (GAG^CGG), MspA1I* (CMG^CGG) CviJI, HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) SatI, TauI SrfI (GCCC^GGGC) Cac8I Eco72I (CAC^GTG) AjiI* (CAC^GTC), ZraI (GAC^GTC) AjiI, MaeII, TaiI AjiI* (GAC^GTG), Ppu21I* (YAC^GTG) Eco72I, MaeII, Ppu21I, TaiI Ecl136II (GAG^CTC), MbiI* (CCG^CTC) MnlI FastDigest® Eco105I (TAC^GTA), Eco105I (TAC^GTA), Ppu21I* (YAC^GTA) MaeII, Ppu21I, TaiI MbiI* (GAG^CGG), MspA1I* (CMG^CGG) HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) BceAI EheI (GGC^GCC) AjiI* (CAC^GTC), ZraI (GAC^GTC) Hin1I AluI (AG^CT), Bsh1236I (CG^CG), Bsp68I (TCG^CGA), CviJI* (RG^CT), HpyCH4V (TG^CA), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG) BsuRI, FastDigest® BsuRI, CviJI Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC), DpnI (GA^TC), PsiI (TTA^TAA) CviJI BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), MlsI (TGG^CCA) BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI Ecl136II (GAG^CTC), MbiI* (CCG^CTC) BsuRI, FastDigest® BsuRI, CviJI, MnlI FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC) LweI Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT) FastDigest® HhaI, HhaI, Hin6I FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA) FastDigest® HhaI, HhaI, Hin6I MbiI* (GAG^CGG), MspA1I* (CMG^CGG) BsuRI, FastDigest® BsuRI, CviJI, HpaII, FastDigest® MspI, MspI PdiI (GCC^GGC) SatI, TauI SrfI (GCCC^GGGC) Cac8I
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Updated sausio 31, 2008 08:52
