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Newly Generated Recognition Sequences Resulting from:

• Ligation of Blunt DNA Ends (see below)

• Ligation of Protruding Compatible DNA Ends
• Fill-in of a 5'-overhang and Self-ligation
• Removal of a 3'-overhang and Self-ligation

Newly Generated Recognition Sequences Resulting from the Ligation of Blunt DNA Ends (A-B)

A-B    C-D-E    F-G-H-I-K-L    M-N-O-P-R-S-T-V-X-Z

Note

• Restriction enzymes that have degenerate recognition sequences (i.e., recognize more than one sequence) are indicated by an asterisk (*). Be aware that these restriction enzymes will cleave sequences in addition to the one listed.
• Enzymes produced by Fermentas are shown in green.

Single letter code:
R = G or A;  Y = C or T;  W = A or T;  M = A or C;	K = G or T;  S = C or G; H = A, C or T; V = A, C or G;	B = C, G or T; D = A, G or T; N = G, A, T or C.

First restriction enzyme	Second restriction enzyme	Restriction enzymes that cleave the newly generated recognition sequence
AjiI* (CAC^GTC)	Ecl136II (GAG^CTC), MbiI* (CCG^CTC)	MnlI
	FastDigest® Eco105I (TAC^GTA), Eco105I (TAC^GTA), Ppu21I* (YAC^GTA)	MaeII, Ppu21I, TaiI
	Eco72I (CAC^GTG), Ppu21I* (YAC^GTG)	Eco72I, MaeII, Ppu21I, TaiI
	MbiI* (GAG^CGG), MspA1I* (CMG^CGG)	HpaII, FastDigest® MspI, MspI
	PdiI (GCC^GGC)	BceAI
	ZraI (GAC^GTC)	AjiI, MaeII, TaiI
AjiI* (GAC^GTG)	Bsp68I (TCG^CGA)	TaqII
	DpnI (GA^TC)	FastDigest® HinfI, HinfI
	Ecl136II (GAG^CTC), MbiI* (CCG^CTC)	MnlI
	FastDigest® Eco105I (TAC^GTA), Eco105I (TAC^GTA), Eco72I (CAC^GTG), Ppu21I* (YAC^GTA), Ppu21I* (YAC^GTG)	MaeII, TaiI
	Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), FspAI* (RTGC^GCAC), FspAI* (RTGC^GCAT), FastDigest® NsbI (TGC^GCA), NsbI (TGC^GCA)	CseI
	EheI (GGC^GCC)	CseI, Hin1I
	MbiI* (GAG^CGG), MspA1I* (CMG^CGG)	HpaII, FastDigest® MspI, MspI
	PdiI (GCC^GGC)	BceAI
	ZraI (GAC^GTC)	AatII, Hin1I, MaeII, TaiI, ZraI
AluI (AG^CT)	Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC)	Csp6I, FastDigest® RsaI, RsaI
	BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MlsI (TGG^CCA)	CviJI
	CviJI* (RG^CT), Ecl136II (GAG^CTC), MbiI* (CCG^CTC), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG)	AluI, CviJI
	FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC)	Bsp143I, DpnI, MboI
	EheI (GGC^GCC)	BsuRI, FastDigest® BsuRI, CviJI
Bsh1236I (CG^CG)	Bsp68I (TCG^CGA), MbiI* (GAG^CGG), MspA1I* (CMG^CGG)	Bsh1236I
	Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC)	Csp6I, FastDigest® RsaI, RsaI
	FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC)	Bsp143I, DpnI, MboI
	Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT)	CviJI
	EheI (GGC^GCC)	BsuRI, FastDigest® BsuRI, CviJI
Bsp68I (TCG^CGA)	Bsh1236I (CG^CG), MbiI* (GAG^CGG), MspA1I* (CMG^CGG)	Bsh1236I
	Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC)	Csp6I, FastDigest® RsaI, RsaI
	DraI (TTT^AAA), FastDigest® DraI (TTT^AAA), FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC), MssI (GTTT^AAAC), FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT), SmiI (ATTT^AAAT), FastDigest® SmiI (ATTT^AAAT), SspI (AAT^ATT), FastDigest® SspI (AAT^ATT)	TaqI, FastDigest® TaqI
	FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC)	Bsp143I, DpnI, MboI, TaqI, FastDigest® TaqI
	Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT)	CviJI
	EheI (GGC^GCC)	BsuRI, FastDigest® BsuRI, CviJI
	FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC)	Hpy188I
Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC)	AluI (AG^CT), Bsh1236I (CG^CG), Bsp68I (TCG^CGA), BsuRI (GG^CC), FastDigest® BsuRI (GG^CC), CviJI* (RG^CC), CviJI* (RG^CT), Ecl136II (GAG^CTC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), HpyCH4V (TG^CA), MbiI* (CCG^CTC), MbiI* (GAG^CGG), MlsI (TGG^CCA), MspA1I* (CMG^CGG), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG)	Csp6I, FastDigest® RsaI, RsaI
	Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT)	AluI, CviJI
	EheI (GGC^GCC)	CviJI
	FastDigest® HincII* (GTY^AAC), HincII* (GTY^AAC), KspAI (GTT^AAC), FastDigest® KspAI (GTT^AAC)	FastDigest® Hpy8I, Hpy8I
	FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC)	FastDigest® Hpy8I, Hpy8I, FastDigest® XmiI, XmiI
	FastDigest® RsaI (GT^AC), RsaI (GT^AC), ScaI (AGT^ACT)	MaeIII
	SspI (AAT^ATT), FastDigest® SspI (AAT^ATT)	TasI
BsuRI (GG^CC), FastDigest® BsuRI (GG^CC)	AluI (AG^CT), CviJI* (RG^CT), Ecl136II (GAG^CTC), Eco47III (AGC^GCT), FastDigest® Eco47III (AGC^GCT), MbiI* (CCG^CTC), MspA1I* (CMG^CTG), PvuII (CAG^CTG), FastDigest® PvuII (CAG^CTG)	CviJI
	Bst1107I (GTA^TAC), FastDigest® Bst1107I (GTA^TAC)	Csp6I, FastDigest® RsaI, RsaI
	CviJI* (RG^CC), Eco147I (AGG^CCT), FastDigest® Eco147I (AGG^CCT), MlsI (TGG^CCA)	BsuRI, FastDigest® BsuRI, CviJI
	FastDigest® Eco32I (GAT^ATC), Eco32I (GAT^ATC)	Bsp143I, BspPI, DpnI, MboI
	EheI (GGC^GCC)	BmgT120I, BsuRI, FastDigest® BsuRI, Cfr13I, FastDigest® Cfr13I, CviJI
	FspAI* (RTGC^GCAC)	BseSI, SduI
	FastDigest® HincII* (GTY^GAC), HincII* (GTY^GAC)	FaqI

catalog@fermentas.com

Updated sausio 31, 2008 08:52