Restriction Enzymes

• Fermentas - the Leader in Restriction Enzyme Quality, flyer in pdf, 183 KB

  Products
• An Innovation from Fermentas: FastDigest® Restriction Enzymes
• Unique Restriction Enzymes
• Certificates of Analysis
• MSDS
• Support:
  • Introduction. Classification
  • Alphabetic Lists
  • Quality Guarantee & Control
  • Description of Icons
  • General Properties of Restriction Enzymes
  • Troubleshooting
  • Information on New Cleavage Sites
  • Number of Recognition Sites in DNA Molecules
• REsearch™ improved
• DoubleDigest™
• REviewer™ new
• Frequently Asked Questions

2008-2009 Catalog & Product Application Guide
FastDigest® & CONVENTIONAL RESTRICTION ENZYMES, 7.28 MB

 Support:

• Introduction. Classification of Restriction Enzymes
• Alphabetic Lists of:
  • Fermentas Conventional Restriction Enzymes and Cross-reference to FastDigest® Enzymes
  • Commercially Available Restriction Enzymes  
  • Recognition Specificities
• Commercial Restriction Enzymes Sorted by the Type of DNA Ends Generated
  • Enzymes Generating 5'-protruding Ends
  • Enzymes Generating 3'-protruding Ends
  • Enzymes Generating Blunt Ends
  • Enzymes Cleaving DNA on Both Sides of Their Recognition Sequence
• Quality Guarantee & Control
  • PureExtreme® Quality Guarantee
  • Activity Assay
  • Quality Control
  • Storage and Shipping
• Description of Icons
• General Properties of Restriction Enzymes:
  • Reaction Conditions for Restriction Enzymes
    • General Protocol for DNA Digestion
    • Five Buffer System
    • Dilution of Restriction Enzymes
    • Stability During Prolonged Incubation
    • Inactivation
    • Considerations for Partial Digestion of DNA
    • Chart: Reaction Conditions for Restriction Enzymes and poster in PDF format, 721 MB
  • How do I Perform Double Digest?
  • Activity of Mesophilic and Thermophilic Enzymes at 37°C
  • Site Preferences by Restriction Enzymes
  • Star Activity (Relaxation of Specificity)
  • Digestion of Methylated DNA
    • Effect of Dam and Dcm Methylation on DNA Cleavage by Restriction Enzymes
    • Effect of CpG Methylation on DNA Cleavage by Restriction Enzymes
    • Effect of EcoKI and EcoBI Methylation on DNA Cleavage by Restriction Enzymes
  • Cleavage of Restriction Targets Located in Close Vicinity within pUC19 Multiple Cloning Site
  • Digestion of PCR Products
    • Cleavage of PCR Products Directly After Amplification Reaction
    • Cleavage Efficiency Close to the Termini of PCR Fragments
• Fermentas Guide for Successful Digestions:
  • No digestion or incompletely digested DNA
  • Atypical cleavage pattern
  • Diffused DNA zones, gel shift
  • Low efficiency of restriction fragment ligation
• Information on New Cleavage Sites
  • Newly Generated Recognition Sequences Resulting from the Ligation of Blunt DNA Ends
  • Newly Generated Recognition Sequences Resulting from the Ligation of Protruding Compatible DNA Ends
  • Newly Generated Recognition Sequences Resulting from the Fill-in of a 5'-overhang and Self-ligation
  • Newly Generated Restriction Sequences Resulting from the Removal of a 3'-overhang and Self-ligation
• Number of Recognition Sites in DNA Molecules

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