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GenBank/EMBL accession number Y14837.
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in pUC57 DNA for the following enzymes:
AarI, AdeI, AloI, AscI, BaeI, BbvCI, BclI, BcuI, BglII, BoxI, BpiI, BplI, Bpu10I, Bpu1102I, BsaAI, BseJI, BseRI, BsgI, BshTI, Bsp119I, Bsp1407I, BspTI, Bst1107I, BstXI, Bsu15I, BtgI, BtrI, BveI, Cfr42I, CpoI, Eco47III, Eco52I, Eco72I, Eco81I, Eco91I, Eco105I, Eco130I, FalI, FaqI, FseI, FspAI, Kpn2I, KspAI, MlsI, MluI, MssI, MunI, NcoI, NheI, NotI, OliI, PacI, PauI, PdiI, Pfl23II, PsiI, Psp5II, PsrI, PsyI, SanDI, SdaI, SexAI, SfiI, SgfI, SgrAI, SmiI, SrfI, TaqII, Van91I, XagI, XcmI, XhoI, XmaJI.A B C D E H K M N P R S X
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 91 932 AatII 2641 Acc65I 408 AflIII 830 AlfI 455 Alw21I 177 402 1144 2305 2390 Alw26I 51 1790 2555 2708 Alw44I 177 1144 2390 ApaI 442 BamHI 435 BccI 1759 1883 2170 BceAI 387 1316 BcgI 2239 BcnI 47 82 439 440 1209 1905 2256 BfiI 364 1768 BfmI 453 1095 1286 1964 BfuI 1039 2566 BglI 245 1837 Bme1390I 47 82 354 439 440 569 857 978 991 1209 1905 2256 BpuEI 936 1198 1475 2343 BsaWI 1036 1183 2014 BsaXI 683 BseDI 354 439 569 990 BseGI 77 321 1703 1884 2171 BseLI 47 672 846 864 1030 1309 BseMI 1777 1959 BseMII 171 1105 1514 1680 2220 BseNI 365 391 630 1233 1246 1363 1769 1887 1930 2194 2369 BseSI 177 442 1144 2390 BseXI 41 254 327 654 735 753 1172 1237 1240 1446 1774 2140 BseYI 1134 Bsh1236I 2 4 107 415 676 678 876 1457 1787 2280 2612 Bsh1285I 276 743 1167 2090 2239 BshNI 235 408 574 1671 Bsp68I 414 Bsp120I 442 BspLI 235 408 435 442 574 860 899 1671 1765 1806 2017 2607 BspLU11I 830 BspPI 435 436 1397 1471 1483 1568 1581 2045 2348 2366 BssSI 1003 2387 2694 BstAPI 179 BtsI 617 2116 2144 CaiI 1241 CfrI 388 669 2111 Cfr9I 439 Cfr10I 1803 Cfr13I 286 442 443 1765 1844 1861 2083 2699 Csp6I 168 409 2202 DdeI 171 1105 1514 1680 2220 2646 DraI 1587 1606 2298 DrdI 91 932 Eam1104I 290 708 2512 Eam1105I 1718 EciI 902 1048 1876 Ecl136II 402 Eco24I 402 442 Eco31I 1790 Eco32I 429 Eco47I 1861 2083 Eco57I 1357 2405 Eco57MI 1357 1808 2405 Eco88I 439 Eco147I 459 EcoO109I 2698 EcoRI 396 EheI 235 Esp3I 51 2707 FspBI 426 1325 1578 1913 GsuI 1808 HgaI 108 932 1510 2260 Hin1I 235 2259 2641 Hin1II 38 466 485 831 1551 2042 2052 2130 2166 2559 2664 Hin4I 429 1718 1792 HincII 448 HindIII 471 HinfI 665 730 805 1201 1718 HphI 12 21 1574 1801 2197 2423 2438 Hpy8I 177 448 1144 1632 2390 Hpy99I 372 385 447 931 1725 1988 Hpy188I 31 156 433 942 1020 1373 1507 1642 2088 2099 2219 KpnI 408 LweI 78 153 208 229 422 918 1970 2180 2410 MaeIII 57 348 368 1187 1250 1366 1649 1980 2038 2191 2379 MbiI 520 761 2562 MboII 291 709 1328 1480 1571 2326 2404 2513 MmeI 1020 1204 Mph1103I 420 MslI 1971 2130 2489 MspA1I 112 306 652 1170 1415 2356 MvaI 354 569 857 978 991 Mva1269I 418 NdeI 183 NmuCI 57 368 1980 2191 NsbI 256 1943 PaeI 465 PagI 1550 2558 2663 PdmI 2318 PfeI 665 805 PfoI 46 PpiI 1521 2364 Psp1406I 1948 2321 PsuI 435 1471 1482 1568 1580 2348 2365 PstI 453 PvuI 276 2090 PvuII 306 652 RsaI 168 409 2202 SacI 402 SalI 448 SapI 707 ScaI 2201 SchI 730 1201 1718 SduI 177 402 442 1144 2305 2390 SmaI 439 SmlI 936 1198 1475 2343 SmuI 114 124 284 622 679 SspI 2525 TaaI 19 54 262 792 863 1333 1646 2161 TaiI 374 1533 1949 2322 2642 TaqI 400 449 930 2374 TasI 397 511 528 603 1591 1897 2152 TatI 167 2201 TauI 150 756 874 1029 2113 2235 2464 TseI 41 254 327 654 735 753 1172 1237 1240 1446 1774 2140 TspDTI 663 1599 1701 2004 TspGWI 2173 2490 VspI 600 659 1894 XapI 396 XbaI 425 XceI 37 65 830 XmiI 448
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Updated liepos 16, 2007 09:23
