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Not available from Fermentas
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in pTZ57R DNA for the following enzymes:
AarI, AatII, BaeI, BbvCI, BclI, BcuI, BglII, BoxI, BpiI, BplI, Bpu10I, Bpu1102I, BseJI, BseRI, BsgI, BshTI, Bsp119I, Bsp1407I, BspTI, Bst1107I, BstAPI, BstXI, Bsu15I, BtgI, BtrI, BveI, Cfr42I, CpoI, Eco47III, Eco52I, Eco72I, Eco81I, Eco91I, Eco105I, Eco130I, EcoO109I, EheI, Esp3I, FalI, FaqI, FseI, FspAI, Kpn2I, KspAI, MlsI, MluI, MssI, MunI, NcoI, NdeI, NheI, NotI, OliI, PacI, PauI, Pfl23II, PfoI, Psp5II, PsyI, SanDI, SexAI, SfiI, SgfI, SgrAI, SgsI, SmiI, SrfI, TaqII, TstI, Van91I, XagI, XcmI, XhoI, XmaJI.A B C D E H K M N P R S X
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 273 1178 Acc65I 627 AdeI 230 AflIII 1076 AlfI 674 AloI 279 Alw21I 621 1390 2551 2636 Alw26I 2036 2801 Alw44I 1390 2639 ApaI 661 BamHI 654 BccI 223 241 2005 2129 2416 BceAI 189 606 1562 BcgI 2485 BcnI 658 659 1455 2151 2502 BfiI 583 2014 BfmI 9 672 699 1341 1532 2210 BfuI 1285 2812 BglI 464 2083 Bme1390I 573 658 659 815 1103 1224 1237 1455 2151 2502 BpuEI 1182 1444 1721 2589 BsaAI 230 BsaWI 1282 1429 2260 BsaXI 279 929 BseDI 573 658 815 1236 BseGI 540 1949 2130 2417 BseLI 7 333 918 1092 1110 1276 1555 BseMI 2023 2205 BseMII 1351 1760 1926 2466 BseNI 316 584 610 876 1479 1492 1609 2015 2133 2176 2440 2615 BseSI 661 1390 2636 BseXI 50 473 546 900 981 999 1418 1483 1486 1692 2020 2386 BseYI 1380 Bsh1236I 3 27 47 423 634 922 924 1122 1703 2033 2526 2858 Bsh1285I 495 989 1413 2336 2485 BshNI 191 627 820 1917 Bsp68I 633 Bsp120I 661 BspPI 654 655 1643 1717 1729 1814 1827 2291 2594 2612 BssSI 1249 2633 BtsI 863 2362 2390 CaiI 1487 CfrI 607 915 2357 Cfr9I 658 Cfr10I 127 2049 Cfr13I 238 505 661 662 2011 2090 2107 2329 CseI 1 1178 1756 2506 Csp6I 628 2448 DraI 1833 1852 2544 Eam1104I 509 954 2758 Eam1105I 1964 EciI 1148 1294 2122 Ecl136II 621 Eco24I 157 621 661 Eco31I 2036 Eco32I 648 Eco47I 2107 2329 Eco57I 1603 2651 Eco57MI 1603 2054 2651 Eco88I 658 Eco147I 678 EcoRI 615 EcoRII 573 815 1103 1224 1237 FspBI 79 645 1571 1824 2159 GsuI 2054 Hin1I 2505 Hin1II 685 731 1077 1797 2288 2298 2376 2412 2805 Hin4I 648 1964 2038 HincII 667 HindIII 690 HinfI 280 302 706 911 976 1051 1447 1964 HpaII 128 659 794 1283 1430 1456 1646 2050 2084 2151 2261 2503 HphI 220 1820 2047 2443 2669 2684 Hpy8I 227 282 299 667 1390 1878 2636 Hpy99I 591 604 666 1177 1971 2234 Hpy188I 173 652 1188 1266 1619 1753 1888 2334 2345 2465 Hpy99I 588 633 644 880 1309 1443 1541 1639 1722 1796 2055 2804 HpyF3I 1351 1760 1926 2466 KpnI 627 LweI 641 1164 2216 2426 2656 MaeIII 43 55 567 587 1433 1496 1612 1895 2226 2284 2437 2625 MbiI 86 766 1007 2808 MboII 101 510 955 1574 1726 1817 2572 2650 2759 MmeI 276 1266 1450 Mph1103I 639 MslI 2217 2376 2735 MspI 128 659 794 1283 1430 1456 1646 2050 2084 2151 2261 2503 MspA1I 525 898 1416 1661 2602 Mva1269I 637 MvaI 573 815 1103 1224 1237 NlaIII 685 731 1077 1797 2288 2298 2376 2412 2805 NmuCI 55 587 2226 2437 NsbI 475 2189 PaeI 684 PagI 1796 2804 PdiI 127 PdmI 2564 PpiI 280 1767 2610 PscI 1076 PsiI 358 Psp1406I 2194 2567 PsuI 654 1717 1728 1814 1826 2594 2611 PstI 672 PvuI 495 2336 PvuII 525 898 RsaI 628 2448 SacI 621 SalI 667 SapI 953 ScaI 2447 SchI 280 302 706 976 1447 1964 SduI 157 621 661 1390 2551 2636 SmaI 658 SmlI 1182 1444 1721 2589 SmuI 31 85 503 868 925 SspI 438 2771 TaaI 256 481 1038 1109 1579 1892 2407 TaiI 121 231 274 286 593 1779 2195 2568 TaqI 197 619 668 1176 2620 TasI 416 427 453 616 757 774 849 1837 2143 2398 TatI 2447 TauI 14 28 1002 1120 1275 2359 2481 2710 TfiI 911 1051 TseI 50 473 546 900 981 999 1418 1483 1486 1692 2020 2386 VspI 846 905 2140 XapI 415 426 615 XbaI 644 XceI 684 1076 XmiI 667
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Updated liepos 16, 2007 09:23
