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pTZ19R
GenBank/EMBL accession number Y14835.pTZ19U
GenBank/EMBL accession number Y14836.Not available from Fermentas
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in pTZ19R DNA for the following enzymes:
AarI, AatII, AlfI, ApaI, BaeI, BbvCI, BclI, BcuI, BglII, BoxI, BpiI, BplI, Bpu10I, Bpu1102I, BseJI, BseRI, BsgI, BshTI, Bsp68I, Bsp119I, Bsp120I, Bsp1407I, BspTI, Bst1107I, BstAPI, BstXI, Bsu15I, BtgI, BtrI, Cfr42I, CpoI, Eco32I, Eco47III, Eco52I, Eco72I, Eco81I, Eco91I, Eco105I, Eco130I, Eco147I, EcoO109I, EheI, Esp3I, FalI, FaqI, FseI, FspAI, Kpn2I, KspAI, MlsI, MluI, Mph1103I, MssI, MunI, Mva1269I, NcoI, NdeI, NheI, NotI, OliI, PacI, PauI, Pfl23II, PfoI, Psp5II, PsrI, PsyI, SanDI, SexAI, SfiI, SgfI, SgrAI, SgsI, SmiI, SrfI, TaqII, TstI, Van91I, XagI, XcmI, XhoI, XmaJI.
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 273 1154 Acc65I 627 AdeI 230 AflIII 1052 AloI 279 Alw21I 621 1366 2527 2612 Alw26I 2012 2777 Alw44I 1366 2612 BamHI 636 BccI 223 241 1981 2105 2392 BceAI 189 606 1538 BcgI 2461 BcnI 631 632 1431 2127 2478 BfiI 583 1990 BfmI 9 654 675 1317 1508 2186 BfuI 1261 2788 BglI 464 2059 Bme1390I 573 631 632 791 1079 1200 1213 1431 2127 2478 BpuEI 1158 1420 1697 2565 BsaAI 230 BsaWI 1258 1405 2236 BsaXI 279 905 BseDI 573 631 632 791 1212 BseGI 540 1925 2106 2393 BseLI 7 333 894 1068 1086 1252 1531 BseMI 1999 2181 BseMII 1327 1736 1902 2442 BseNI 316 584 610 852 1455 1468 1585 1991 2109 2152 2416 2591 BseSI 1366 2612 BseXI 50 473 546 876 957 975 1394 1459 1462 1668 1996 2362 BseYI 1356 Bsh1236I 3 27 47 423 898 900 1098 1679 2009 2502 2834 Bsh1285I 495 965 1389 2312 2461 BshNI 191 627 796 1893 BspPI 636 637 1619 1693 1705 1790 1803 2267 2570 2588 BssSI 1225 2609 BsuRI 239 381 506 608 892 1066 1077 1095 1529 1987 2067 2334 BtsI 839 2338 2366 BveI 652 CaiI 1463 CfrI 607 891 2333 Cfr9I 631 Cfr10I 127 2025 Cfr13I 238 505 1987 2066 2083 2305 CseI 1 1154 1732 2482 Csp6I 628 2424 CviRI 548 655 663 878 1367 1663 1998 2088 2281 2369 2613 DraI 1809 1828 2520 Eam1104I 509 930 2734 Eam1105I 1940 EciI 1124 1270 2098 Ecl136II 621 Eco24I 157 621 Eco31I 2012 Eco47I 2083 2305 Eco57I 1579 2627 Eco57MI 1579 2030 2627 Eco88I 631 EcoRI 615 EcoRII 573 791 1079 1200 1213 FspBI 79 643 1547 1800 2135 GsuI 2030 Hin1I 2481 Hin1II 661 707 1053 1773 2264 2274 2352 2388 2781 Hin4I 1940 2014 HincII 648 HindIII 666 HinfI 280 302 646 682 887 952 1027 1423 1940 HpaII 128 632 770 1259 1406 1432 1622 2026 2060 2127 2237 2479 HphI 220 1796 2023 2419 2645 2660 Hpy8I 227 282 299 648 1366 1854 2612 Hpy99I 591 604 1153 1947 2210 Hpy188I 173 1164 1242 1595 1729 1864 2310 2321 2441 Hpy188III 588 642 856 1285 1419 1517 1615 1698 1772 2031 2780 HpyF3I 1327 1736 1902 2442 KpnI 627 LweI 1140 2192 2402 2632 MaeIII 43 55 567 587 1409 1472 1588 1871 2202 2260 2413 2601 MbiI 86 742 983 2784 MboII 101 510 931 1702 1793 2548 2626 2735 MmeI 276 1242 1426 MnlI 195 508 640 908 941 1167 1224 1491 1891 1972 2102 2308 MslI 2193 2352 2711 MspI 128 632 770 1259 1406 1432 1622 2026 2060 2127 2237 2479 MspAI 525 874 1392 1637 2578 MvaI 573 791 1079 1200 1213 NmuCI 55 587 2202 2413 NsbI 475 2165 PaeI 660 PagI 1772 2780 PdiI 127 PdmI 2540 PfeI 887 1027 PpiI 280 1743 2586 PscI 1052 PsiI 358 Psp1406I 2170 2543 PsuI 636 1693 1704 1790 1802 2570 2587 PstI 654 PvuI 495 2312 PvuII 525 874 RsaI 628 2424 SacI 621 SalI 648 SapI 929 ScaI 2423 SchI 280 302 646 682 952 1423 1940 SdaI 653 SduI 157 621 1366 2527 2612 SmaI 631 SmlI 1158 1420 1697 2565 SmuI 31 85 503 844 901 SspI 438 2747 TaaI 256 481 1014 1085 1555 1868 2383 TaiI 121 231 274 286 593 1755 2171 2544 TaqI 197 619 649 1152 2596 TasI 416 427 453 616 733 750 825 1813 2119 2374 TatI 2423 TauI 14 28 978 1096 1251 2335 2457 2686 TseI 50 473 546 876 957 975 1394 1459 1462 1668 1996 2362 TspDTI 885 1821 1923 2226 TspGWI 2395 2712 VspI 822 881 2116 XapI 415 426 615 XbaI 642 XceI 660 1052 XmiI 648
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Updated liepos 16, 2007 09:24
