pJET1 DNA Restriction Sites
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in pJET1 DNA for the following enzymes:
AarI, Acc65I, AjiI, AjuI, AlfI, BaeI, BclI, BcuI, BoxI, BplI, Bpu1102I, BseJI, BseRI, BsgI, BshTI, Bsp68I, Bsp1407I, Bst1107I, BstAPI, BtgZI, Cfr42I, CpoI, CspCI, Eco81I, Eco91I, Eco105I, Eco147I, EheI, FalI, FseI, FspAI, KpnI, KspAI, MlsI, MluI, Mph1103I, NdeI, OliI, PacI, PasI, PauI, PdiI, Pfl23II, PsiI, Psp5II, PsrI, PsyI, SanDI, SdaI, SexAI, SfiI, SgfI, SgrAI, SgsI, SrfI, TstI, Van91I, XagI, XcmI, XmaJI.A B C D E F G H K LM N P R STX
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 91 1350 AatII 863 3059 AdeI 291 AflIII 172 876 1248 AloI 455 Alw21I 162 1562 2723 2808 Alw26I 51 765 866 2208 2973 3126 Alw44I 1562 2808 ApaI 1030 BamHI 1023 BauI 1421 2805 3112 BbvCI 843 BccI 466 473 2177 2301 2588 BceAI 1734 BcgI 2657 BcnI 47 82 1027 1028 1627 2323 2674 BdaI 422 982 1330 BfiI 2186 BfmI 1036 1513 1704 2382 BfuI 1457 2984 BglI 2255 BglII 509 Bme1390I 47 82 964 1027 1028 1275 1396 1409 1627 2323 2674 BpiI 229 Bpu10I 843 BpuEI 565 1354 1616 1893 2761 BsaWI 469 1454 1601 2432 BsaXI 1101 BseDI 534 964 1027 1408 BseGI 77 467 472 819 2121 2302 2589 BseLI 47 1090 1264 1282 1448 1727 BseMI 2195 2377 BseMII 526 763 844 1523 1932 2098 2638 BseNI 1048 1651 1664 1781 2187 2305 2348 2612 2787 BseSI 1030 1562 2808 BseXI 41 530 1038 1072 1153 1171 1590 1655 1658 1864 2192 2558 BseYI 1552 Bsh1236I 2 4 107 159 1094 1096 1294 1875 2205 2698 3030 Bsh1285I 155 1161 1585 2508 2657 BshNI 969 2089 Bsp119I 181 Bsp120I 1030 BspPI 1023 1024 1815 1889 1901 1986 1999 2463 2766 2784 BspTI 141 BstXI 466 Bsu15I 543 BsuRI 156 1031 1088 1262 1273 1291 1725 2183 2263 2530 3117 BtgI 534 BtsI 1012 2534 2562 BveI 390 Cac8I 136 146 150 160 748 1068 1177 1263 1300 1860 2251 CaiI 1659 CfrI 155 1087 2529 Cfr9I 1027 Cfr10I 2221 Cfr13I 1030 1031 2183 2262 2279 2501 3117 CseI 108 1350 1928 2678 Csp6I 2620 DraI 239 787 881 888 2005 2024 2716 Eam1104I 1126 2930 Eam1105I 2136 EciI 1320 1466 2294 Ecl136II 162 Eco24I 162 1030 Eco31I 2208 Eco32I 495 Eco47I 2279 2501 Eco47III 1040 Eco52I 155 Eco57I 224 753 1775 2823 Eco57MI 224 753 1775 2226 2823 Eco72I 173 Eco88I 478 1027 Eco130I 534 EcoO109I 1030 3116 EcoP15I 1656 1772 1865 EcoRI 178 EcoRII 964 1275 1396 1409 Esp3I 51 865 3125 FaqI 274 FspBI 147 504 1743 1996 2331 GsuI 2226 Hin1I 863 2677 3059 Hin4I 254 2136 2210 HincII 168 HindIII 750 HinfI 663 1083 1148 1223 1619 2136 HphI 12 21 1992 2219 2615 2841 2856 Hpy8I 168 1562 2050 2808 Hpy99I 167 1349 2143 2406 Hpy188I 31 223 1360 1438 1791 1925 2060 2506 2517 2637 HpyCH4V 40 722 835 1037 1074 1563 1859 2194 2284 2477 2565 2809 HpyF3I 247 526 763 844 1523 1932 2098 2638 3064 HpyF10VI 963 1007 1032 1114 1181 1295 1867 2255 Kpn2I 469 LguI 1125 LweI 78 287 818 1336 2388 2598 2828 MaeIII 57 1605 1668 1784 2067 2398 2456 2609 2797 MbiI 915 1179 2980 MboII 230 233 507 551 797 1127 1898 1989 2744 2822 2931 MmeI 1438 1622 MslI 414 467 2389 2548 2907 MspA1I 112 528 1070 1588 1833 2774 MssI 887 MunI 1018 MvaI 964 1275 1396 1409 Mva1269I 848 NcoI 534 NheI 146 NmuCI 57 2398 2609 NotI 154 NsbI 2361 PaeI 150 PagI 668 1968 2976 3081 PdmI 2736 PfeI 1083 1223 PfoI 46 PpiI 1939 2782 Ppu21I 173 PscI 876 1248 Psp1406I 2366 2739 PspXI 477 PstI 1036 PsuI 509 1023 1889 1900 1986 1998 2766 2783 PvuI 2508 PvuII 528 1070 RsaI 2620 SacI 162 SalI 168 ScaI 2619 SchI 663 1148 1619 2136 SduI 162 1030 1562 2723 2808 SmaI 1027 SmiI 238 786 SmoI 141 478 565 1354 1616 1893 2761 SmuI 114 124 1097 SspI 520 687 2943 TaaI 19 54 629 1210 1281 1751 2064 2579 TaiI 174 864 1951 2367 2740 3060 TaqI 169 182 351 479 544 661 1348 2792 TatI 2619 TauI 154 157 1174 1292 1447 2531 2653 2882 TseI 41 530 1038 1072 1153 1171 1590 1655 1658 1864 2192 2558 TsoI 612 2550 TspDTI 235 259 400 412 670 1081 2017 2119 2422 TspGWI 2591 2908 TspRI 1012 1144 1650 1663 1934 2083 2188 2535 2562 VspI 995 1077 2312 XapI 178 242 772 784 790 XbaI 503 XceI 37 150 876 1248 XhoI 478 XmiI 168
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Updated liepos 16, 2007 09:24
