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GenBank/EMBL accession numbers J01749, K00005, L08654, M10282, M10283, M10286, M10356, M10784, M10785, M10786, M33694, V01119.
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in pBR322 DNA for the following enzymes:
AarI, Acc65I, AdeI, AloI, ApaI, BaeI, BbvCI, BclI, BcuI, BglII, BplI, Bpu1102I, BsaXI, BseRI, BshTI, Bsp119I, Bsp120I, Bsp1407I, BspTI, BstXI, BtrI, Cfr9I, Cfr42I, CpoI, Ecl136II, Eco72I, Eco81I, Eco91I, Eco105I, Eco147I, FalI, FseI, KpnI, KspAI, MluI, Mph1103I, MssI, MunI, NcoI, NotI, OliI, PacI, PauI, Pfl23II, PsiI, PsrI, SacI, SanDI, SdaI, SexAI, SfiI, SgfI, SgsI, SmaI, SmiI, SrfI, TaqII, XbaI, XcmI, XhoI, XmaJI.
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 2164 2575 AatII 4284 AflIII 2473 AlfI 1026 1457 1678 Alw21I 276 587 1174 1465 2289 2787 3948 4033 Alw26I 2122 3433 4198 Alw44I 2289 2787 4033 BamHI 375 BccI 458 552 988 1078 1385 1397 3402 3526 3813 BceAI 594 1151 2959 BcgI 708 2063 3882 BcnI 170 534 1258 1484 1812 2118 2153 2852 3548 3899 BfiI 304 610 679 2219 3411 BfmI 138 2738 2929 3607 BfuI 2682 4209 BglI 929 1163 3480 BoxI 712 BpiI 737 1600 4351 Bpu10I 1580 BpuEI 664 870 2579 2841 3118 3986 BsaAI 2225 BsaWI 693 1664 2679 2826 3657 BseDI 115 129 528 534 1167 1369 1447 2633 BseGI 112 133 987 1032 1681 1770 1848 2007 2148 3346 3527 3814 BseJI 1668 BseMI 3420 3602 BseMII 1581 1743 2283 2748 3157 3323 3863 BseSI 2289 2787 4033 BseYI 949 2777 BsgI 1650 Bsh1285I 286 653 939 2386 2810 3733 3882 BshNI 76 119 413 434 548 766 1205 1289 3314 Bsp68I 972 BspPI 375 376 1097 1667 3040 3114 3126 3211 3224 3688 3991 4009 BssSI 2646 4030 4337 Bst1107I 2244 BstAPI 1045 2291 Bsu15I 23 BtgI 528 1447 BtsI 3759 3787 BveI 1063 CaiI 2884 CfrI 295 399 531 939 1444 3754 Cfr10I 160 401 410 769 929 1283 3446 CseI 390 649 944 976 1240 1390 2002 2179 2575 3153 3903 Csp6I 164 2280 3845 DraI 3230 3249 3941 Eam1104I 2351 4155 Eam1105I 3361 EciI 1393 2545 2691 3519 Eco24I 471 485 Eco31I 3433 Eco32I 185 Eco47I 799 887 1136 1439 1481 1760 3504 3726 Eco47III 232 494 775 1727 Eco52I 939 Eco57I 3000 4048 Eco57MI 811 1401 1981 3000 3451 4048 Eco88I 1425 Eco130I 1369 EcoO109I 523 1438 1480 4341 EcoRI 4359 EcoRII 130 1059 1442 2500 2621 2634 EheI 413 434 548 1205 Esp3I 2122 FaqI 538 888 1084 1761 FspAI 259 1453 FspBI 230 1489 2968 3221 3556 GsuI 811 1401 1981 3451 Hin1I 413 434 548 1205 3902 4284 Hin4I 12 330 1137 3361 3435 HincII 651 3905 HindIII 29 HinfI 632 852 1006 1304 1525 2029 2373 2448 2844 3361 HphI 126 408 453 1307 1528 2083 2092 3217 3444 3840 4066 4081 Hpy8I 651 1787 2244 2289 2787 3275 3905 4033 Hpy99I 650 718 819 943 975 1241 2574 3368 3631 HpyF3I 1581 1743 2283 2748 3157 3323 3863 4289 Kpn2I 1664 MbiI 2404 4205 MboII 464 738 1009 1601 2352 3123 3214 3969 4047 4156 4352 MlsI 1444 MmeI 197 284 2663 2847 MslI 1027 1458 1653 2044 3614 3773 4132 MspA1I 1139 2064 2183 2813 3058 3999 MvaI 130 1059 1442 2500 2621 2634 Mva1269I 1353 NdeI 2295 NheI 229 NmuCI 125 213 881 1148 1915 2128 2223 3623 3834 NsbI 260 1356 1454 3586 PaeI 562 PagI 489 3193 4201 4306 PdiI 401 769 929 1283 PdmI 2029 3961 PfeI 852 1006 1304 1525 2029 2448 PfoI 2117 PpiI 3164 4007 PscI 2473 Psp5II 1438 1480 Psp1406I 900 1799 3591 3964 PsuI 375 1667 3114 3125 3211 3223 3991 4008 PstI 3607 PsyI 2217 PvuI 3733 PvuII 2064 RsaI 164 2280 3845 SalI 651 SapI 2350 ScaI 3844 SchI 632 2373 2844 3361 SduI 276 471 485 587 1174 1465 2289 2787 3948 4033 SgrAI 409 SmlI 664 870 2579 2841 3118 3986 SmuI 181 703 890 1037 1232 1497 1763 1949 2185 2195 SspI 4168 TaiI 901 957 1546 1570 1800 2226 3176 3592 3965 4285 TaqI 24 339 652 1127 1268 2573 4017 TasI 59 252 1320 1334 3234 3540 3795 4360 TatI 2279 3844 TspDTI 86 1523 1593 1821 1861 1882 2009 3242 3344 3647 TspGWI 1301 1610 1903 3816 4133 TstI 389 Van91I 1315 1364 VspI 3537 XagI 622 XapI 4359 XceI 562 1816 2108 2473 XmiI 651 2244
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Updated liepos 16, 2007 09:24
