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pBluescript II SK(-)
GenBank/EMBL accession number X52330.pBluescript II SK(+)
GenBank/EMBL accession number X52328.Not available from Fermentas
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in pBluescript II SK(-) DNA for the following enzymes:
AarI, AatII, AlfI, BaeI, BbvCI, BclI, BglII, BoxI, BpiI, BplI, Bpu10I, Bpu1102I, BseJI, BseRI, BsgI, BshTI, Bsp68I, Bsp119I, Bsp1407I, BspTI, Bst1107I, BstAPI, BtrI, BveI, CpoI, Eco47III, Eco72I, Eco81I, Eco91I, Eco105I, Eco130I, Eco147I, EheI, Esp3I, FalI, FaqI, FseI, FspAI, Kpn2I, KspAI, MlsI, MluI, Mph1103I, MssI, MunI, Mva1269I, NcoI, NdeI, NheI, PacI, PaeI, Pfl23II, PfoI, Psp5II, PsrI, PsyI, SanDI, SdaI, SexAI, SfiI, SgfI, SgrAI, SgsI, SmiI, SrfI, TaqII, TstI, Van91I, XagI, XcmI, XmaJI.
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 275 1255 Acc65I 653 AdeI 232 AflIII 1153 AloI 281 Alw21I 755 1467 2628 2713 Alw26I 2113 2878 Alw44I 1467 2713 ApaI 659 BamHI 719 BccI 225 243 2082 2206 2493 BceAI 191 608 1639 BcgI 2562 BcnI 657 713 714 1532 2228 2579 BcuI 725 BfiI 585 2091 BfmI 11 638 707 1418 1609 2287 BfuI 1362 2889 BglI 466 2160 Bme1390I 575 657 713 714 892 1180 1301 1314 1532 2228 2579 BpuEI 1259 1521 1798 2666 BsaAI 232 BsaWI 1359 1506 2337 BsaXI 281 1006 BseDI 575 713 714 747 892 1313 BseGI 542 2026 2207 2494 BseLI 9 335 664 995 1169 1187 1353 1632 BseMI 2100 2282 BseMII 1428 1837 2003 2543 BseNI 318 586 612 953 1556 1569 1686 2092 2210 2253 2517 2692 BseSI 659 1467 2713 BseYI 1457 Bsh1285I 497 738 1066 1490 2413 2562 BshNI 193 653 897 1994 Bsp120I 659 BspPI 719 720 1720 1794 1806 1891 1904 2368 2671 2689 BssSI 1326 2710 BstXI 744 Bsu15I 683 BtgI 747 BtsI 940 2439 2467 CaiI 1564 CfrI 609 738 992 2434 Cfr9I 713 Cfr10I 129 2126 Cfr13I 240 507 659 660 2088 2167 2184 2406 Cfr42I 747 CseI 3 1255 1833 2583 Csp6I 654 2525 CviRI 550 708 979 1468 1764 2099 2189 2382 2470 2714 DraI 1910 1929 2621 Eam1104I 511 1031 2835 Eam1105I 2041 EciI 1225 1371 2199 Ecl136II 755 Eco24I 159 659 755 Eco31I 2113 Eco32I 695 Eco47I 2184 2406 Eco52I 738 Eco57I 1680 2728 Eco57MI 758 1680 2131 2728 Eco88I 668 713 EcoO109I 659 EcoRI 701 EcoRII 575 892 1180 1301 1314 FspBI 81 726 732 1648 1901 2236 GsuI 758 2131 Hin1I 2582 Hin1II 808 1154 1874 2365 2375 2453 2489 2882 Hin4I 2041 2115 HincII 674 HindIII 689 HinfI 282 304 632 988 1053 1128 1524 2041 HphI 222 1897 2124 2520 2746 2761 Hpy8I 229 284 301 674 1467 1955 2713 Hpy99I 593 606 676 1254 2048 2311 Hpy188I 175 1265 1343 1696 1830 1965 2411 2422 2542 Hpy188III 590 731 957 1386 1520 1618 1716 1799 1873 2132 2881 HpyF3I 1428 1837 2003 2543 KpnI 653 LweI 1241 2293 2503 2733 MaeIII 45 57 569 589 1510 1573 1689 1972 2303 2361 2514 2702 MbiI 88 735 843 1084 2885 MboII 103 512 1032 1803 1894 2649 2727 2836 MmeI 278 1343 1527 MslI 745 2294 2453 2812 MspA1I 527 747 975 1493 1738 2679 MvaI 575 892 1180 1301 1314 NmuCI 57 589 2303 2514 NotI 737 NsbI 477 2266 OliI 745 PagI 1873 2881 PauI 619 792 PdiI 129 PdmI 2641 PfeI 988 1128 PpiI 282 1844 2687 PscI 1153 PsiI 360 Psp1406I 2271 2644 PsuI 719 1794 1805 1891 1903 2671 2688 PstI 707 PvuI 497 2413 PvuII 527 975 RsaI 654 2525 SacI 755 SalI 674 SapI 1030 ScaI 2524 SchI 282 304 632 1053 1524 2041 SduI 159 659 755 1467 2628 2713 SmaI 713 SmlI 668 1259 1521 1798 2666 SmuI 33 87 505 945 1002 SspI 440 2848 TaaI 258 483 678 1115 1186 1656 1969 2484 TaiI 123 233 276 288 595 1856 2272 2645 TaqI 199 669 675 684 699 1253 2697 TasI 418 429 455 648 702 788 834 851 926 1914 2220 2475 TatI 2524 TauI 16 30 737 740 1079 1197 1352 2436 2558 2787 TspDTI 986 1922 2024 2327 TspGWI 2496 2813 VspI 923 982 2217 XapI 417 428 701 XbaI 731 XceI 1153 XhoI 668 XmiI 674
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Updated liepos 16, 2007 09:24
