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pBluescript II KS(-)
GenBank/EMBL accession number X52329.pBluescript II KS(+)
GenBank/EMBL accession number X52327.Not available from Fermentas
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in pBluescript II KS(+) DNA for the following enzymes:
AarI, AatII, AlfI, BaeI, BbvCI, BclI, BglII, BoxI, BpiI, BplI, Bpu10I, Bpu1102I, BseJI, BseRI, BsgI, BshTI, Bsp68I, Bsp119I, Bsp1407I, BspTI, Bst1107I, BstAPI, BtrI, BveI, CpoI, Eco47III, Eco72I, Eco81I, Eco91I, Eco105I, Eco130I, Eco147I, EheI, Esp3I, FalI, FseI, FspAI, Kpn2I, KspAI, MlsI, MluI, Mph1103I, MssI, MunI, Mva1269I, NcoI, NdeI, NheI, PacI, PaeI, Pfl23II, PfoI, Psp5II, PsrI, PsyI, SanDI, SdaI, SexAI, SfiI, SgfI, SgrAI, SgsI, SmiI, SrfI, TaqII, TstI, Van91I, XagI, XcmI, XmaJI.
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 176 1255 Acc65I 755 AdeI 222 AflIII 1153 AloI 169 Alw21I 653 1467 2628 2713 Alw26I 2113 2878 Alw44I 1467 2713 ApaI 749 BamHI 689 BccI 215 233 2082 2206 2493 BceAI 267 608 1639 BcgI 2562 BcnI 695 696 752 1532 2228 2579 BcuI 683 BfiI 585 2091 BfmI 446 638 701 1418 1609 2287 BfuI 1362 2889 BglI 466 2160 Bme1390I 575 695 696 752 892 1180 1301 1314 1532 2228 2579 BpuEI 1259 1521 1798 2666 BsaAI 225 BsaWI 1359 1506 2337 BsaXI 171 1006 BseDI 575 661 694 695 892 1313 BseGI 542 2026 2207 2494 BseLI 117 443 739 995 1169 1187 1353 1632 BseMI 2100 2282 BseMII 1428 1837 2003 2543 BseNI 140 586 612 953 1556 1569 1686 2092 2210 2253 2517 2692 BseSI 749 1467 2713 BseYI 759 1457 Bsh1285I 497 670 1066 1490 2413 2562 BshNI 264 755 897 1994 Bsp120I 749 BspPI 689 690 1720 1794 1806 1891 1904 2368 2671 2689 BssSI 1326 2710 BstXI 658 Bsu15I 725 BtgI 661 BtsI 940 2439 2467 CaiI 1564 CfrI 609 670 992 2434 Cfr9I 695 Cfr10I 328 2126 Cfr13I 218 507 749 750 2088 2167 2184 2406 Cfr42I 661 CseI 455 1255 1833 2583 Csp6I 756 2525 CviRI 550 702 979 1468 1764 2099 2189 2382 2470 2714 DraI 1910 1929 2621 Eam1104I 511 1031 2835 Eam1105I 2041 EciI 1225 1371 2199 Ecl136II 653 Eco24I 298 653 749 Eco31I 2113 Eco32I 713 Eco47I 2184 2406 Eco52I 670 Eco57I 1680 2728 Eco57MI 1680 2131 2728 Eco88I 695 740 EcoO109I 748 EcoRI 707 EcoRII 575 892 1180 1301 1314 FaqI 457 FspBI 378 678 684 1648 1901 2236 GsuI 2131 Hin1I 2582 Hin1II 808 1154 1874 2365 2375 2453 2489 2882 Hin4I 2041 2115 HincII 734 HindIII 719 HinfI 154 176 632 988 1053 1128 1524 2041 HphI 236 1897 2124 2520 2746 2761 Hpy8I 156 173 228 734 1467 1955 2713 Hpy99I 593 606 733 1254 2048 2311 Hpy188I 283 1265 1343 1696 1830 1965 2411 2422 2542 Hpy188III 590 677 957 1386 1520 1618 1716 1799 1873 2132 2881 HpyF3I 1428 1837 2003 2543 KpnI 755 LweI 1241 2293 2503 2733 MaeIII 401 413 569 589 1510 1573 1689 1972 2303 2361 2514 2702 MbiI 369 673 843 1084 2885 MboII 355 512 1032 1803 1894 2649 2727 2836 MmeI 179 1343 1527 MslI 659 2294 2453 2812 MspA1I 527 661 975 1493 1738 2679 MvaI 575 892 1180 1301 1314 NmuCI 401 589 2303 2514 NotI 669 NsbI 477 2266 OliI 659 PagI 1873 2881 PauI 619 792 PdiI 328 PdmI 2641 PfeI 988 1128 PpiI 169 1844 2687 PscI 1153 PsiI 97 Psp1406I 2271 2644 PsuI 689 1794 1805 1891 1903 2671 2688 PstI 701 PvuI 497 2413 PvuII 527 975 RsaI 756 2525 SacI 653 SalI 734 SapI 1030 ScaI 2524 SchI 154 176 632 1053 1524 2041 SduI 298 653 749 1467 2628 2713 SmaI 695 SmlI 740 1259 1521 1798 2666 SmuI 371 425 505 945 1002 SspI 17 2848 TaaI 200 483 731 1115 1186 1656 1969 2484 TaiI 171 183 226 336 595 1856 2272 2645 TaqI 260 711 726 735 741 1253 2697 TasI 4 30 41 648 708 788 834 851 926 1914 2220 2475 TatI 2524 TauI 428 442 669 672 1079 1197 1352 2436 2558 2787 TspDTI 986 1922 2024 2327 TspGWI 2496 2813 VspI 923 982 2217 XapI 29 40 707 XbaI 677 XceI 1153 XhoI 740 XmiI 734
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Updated liepos 16, 2007 09:24
