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GenBank/EMBL accession number X06402.
Not available from Fermentas
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in pACYC177 DNA for the following enzymes:
AarI, Acc65I, AflIII, AloI, ApaI, BaeI, BclI, BcuI, BglII, BoxI, BplI, Bpu1102I, BsaAI, BseJI, BseYI, Bsp120I, Bsp1407I, BspTI, BstAPI, BstXI, BtrI, BveI, CpoI, Ecl136II, Eco32I, Eco52I, Eco72I, Eco81I, Eco105I, Eco130I, EcoRI, EheI, FseI, FspAI, KpnI, Kpn2I, KspAI, MlsI, MluI, MssI, MunI, NcoI, NdeI, NotI, OliI, PacI, PaeI, PauI, PdiI, Pfl23II, PscI, Psp5II, PsrI, PsyI, PvuII, SacI, SalI, SanDI, SapI, SdaI, SexAI, SfiI, SgsI, SmiI, SrfI, TaqII, XbaI, XcmI, XmaJI.A B C D E H K M N P R S X
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 3397 AatII 3563 AdeI 1818 AlfI 2024 AluI 191 254 354 867 1100 1691 2473 2923 3045 3152 3359 3458 Alw21I 3814 3899 Alw26I 474 1147 1826 2365 3650 Alw44I 3814 BamHI 3320 BbvCI 1594 3017 3108 BccI 94 381 505 1650 2126 2170 2611 2963 3284 BceAI 1330 2657 2794 BcgI 18 BcnI 8 359 1023 1120 2226 2227 BfmI 299 BfiI 495 BfuI 3638 BglI 421 BpiI 3496 Bpu10I 1594 2370 3017 3108 3211 BpuEI 782 1033 3861 BsaWI 249 1048 1178 1502 2489 BsaXI 1673 2934 BseDI 885 1224 1339 1677 1738 2225 2226 2627 2874 2935 3392 3476 BseGI 93 380 561 1654 1979 2612 2959 3071 3113 3285 BseLI 1221 1738 2262 2594 2611 2869 3392 3471 BseMI 304 486 1847 BseRI 902 969 BseSI 3429 3814 BseXI 124 301 490 992 995 1060 1508 BsgI 1506 Bsh1236I 478 802 1155 1340 1960 2010 2355 3169 3594 3926 Bsh1285I 24 173 1064 1434 2351 3156 BshNI 592 BshTI 1178 1502 Bsp68I 2009 Bsp119I 852 BspLI 246 457 498 592 1217 1315 1673 2939 3320 3597 BssSI 1211 1606 3006 3510 3817 Bst1107I 1577 Bsu15I 2044 BtgI 1339 1677 1738 2874 2935 3392 3476 BtsI 119 430 1418 1588 2007 2291 2437 3383 3469 Cac8I 297 430 1418 1588 2007 2291 2437 3383 3469 CaiI 987 CfrI 152 1157 3165 3378 Cfr9I 2226 Cfr10I 460 945 1178 1502 2308 Cfr13I 181 403 420 499 1343 1706 2907 3342 3506 Cfr42I 1339 CseI 4 754 1283 1497 3414 Csp6I 63 2188 3251 3389 DraI 657 676 3906 Eam1104I 2161 3692 Eam1105I 540 EciI 387 1312 1393 Eco24I 2005 Eco31I 473 Eco47I 181 403 1706 2907 Eco47III 1591 3021 Eco57I 3799 Eco57MI 455 3799 Eco88I 1952 2226 Eco91I 3279 Eco147I 1636 2976 EcoO109I 3505 EcoRII 1224 1237 1372 1383 1634 2243 2600 2979 Esp3I 2364 FalI 771 FaqI 2791 FspBI 352 687 1589 3325 GsuI 455 Hin1I 4 3563 Hin4I 466 540 1765 2036 2578 2842 3064 3414 HincII 1 HindIII 2472 Hpy8I 1 631 1182 1577 1704 2908 3394 3814 Hpy99I 276 539 3240 3255 3405 HpyF10VI 301 421 998 1159 1409 1638 2060 2092 2306 2969 3099 MbiI 1416 3471 3642 MmeI 1027 1066 1971 2165 2571 2580 3188 Mph1103I 2200 2466 MslI 129 288 3138 3711 MspAI 1061 1166 1339 3848 MvaI 1224 1237 1372 1383 1634 2243 2600 2979 Mva1269I 2238 2315 NheI 1588 NmuCI 73 284 912 2502 3280 NsbI 320 PagI 713 1872 3541 3646 PdmI 1532 3882 PfeI 1642 2265 2321 2493 2584 2971 3368 PfoI 1382 PpiI 736 3834 PsiI 3483 Psp1406I 315 3883 PsuI 683 695 2603 3320 3839 3856 PstI 299 PvuI 173 2351 RsaI 63 2188 3251 3389 ScaI 62 SchI 546 1031 1448 1626 2578 2987 SduI 2005 3429 3814 3899 SgfI 2350 SgrAI 1501 SmaI 2226 SmlI 782 1033 1952 3861 SmuI 3470 SspI 2277 3679 TaiI 316 732 1819 1944 3404 3564 3884 TaqI 853 1429 1799 1953 2045 2319 2722 2815 3155 3832 TatI 62 TauI 29 151 1156 1220 1338 1341 1422 1958 2797 3167 3380 3741 TseI 124 301 490 992 995 1060 1508 TspDTI 260 563 665 1530 1874 2388 2645 2694 2710 3115 3419 TspGWI 91 1740 2148 2185 2873 3442 3715 TstI 1761 2845 Van91I 2611 VspI 369 2552 XagI 2262 XapI 1967 2151 3373 3488 XceI 1013 XhoI 1952 XmiI 1577
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Updated liepos 16, 2007 09:25
