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M13mp18
GenBank/EMBL accession number M77815.Not available from Fermentas
M13mp19
GenBank/EMBL accession number L08821.Not available from Fermentas
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
- *According to our experimental data:
There are no restriction sites in M13mp18 DNA for the following enzymes:
AarI, AatII, ApaI, BcgI, BclI, BcuI, BfuI, BoxI, BpiI, BplI, Bpu1102I, BsgI, BshTI, Bsp68I, Bsp119I, Bsp120I, BspTI, BssSI, Bst1107I, BstAPI, BstXI, BtrI, Cfr42I, CpoI, Eam1105I, Eco31I, Eco32I, Eco52I, Eco57I, Eco72I, Eco91I, Eco130I, Eco147I, EcoO109I, FseI, FspAI, Kpn2I, KspAI, MluI, Mph1103I, MssI, MunI, NcoI, NheI, NotI, OliI*, PauI, Pfl23II, PfoI, Psp5II, PsrI, PsyI, SanDI, SapI, ScaI, SexAI, SfiI, SgfI, SgrAI, SgsI, SrfI, TaqII, Van91I, XagI, XcmI, XhoI, XmaJI.
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 5759 Acc65I 6243 AdeI 5716 AflIII 195 3618 3717 AlfI 195 3618 3717 AloI 5765 Alw21I 4743 5465 6237 Alw26I 2013 2189 4036 5972 7017 Alw44I * 4743 BaeI 1795 2137 6517 BamHI 6252 BbvCI 1370 1416 BceAI 1334 1952 3440 4719 5675 6295 6606 BcnI 1924 6247 6248 6838 BfiI 6317 BfmI 205 1329 1751 2470 5495 6270 6673 BglI 6431 BglII 6935 Bme1390I 1014 1924 1966 5941 5998 6137 6247 6248 6328 6455 6838 Bpu10I 1370 1416 4012 4280 BpuEI 868 2005 2099 2612 5370 BsaAI 1268 4446 5037 5462 5716 BsaWI 313 965 2377 2395 3369 3841 BsaXI 1891 2667 5765 6017 BseDI 2763 2894 5997 6136 6247 6248 6327 6617 6838 BseGI 239 3547 6361 7244 BseJI 1149 3974 BseMI 5198 6913 7067 BseRI 2008 BseSI * 2088 4743 BseXI 932 1367 2521 3132 4871 5536 5923 6052 6355 6428 BseYI 1192 3277 5896 Bsh1285I 1422 3879 6405 6521 BshNI 1249 5677 6001 6131 6243 6465 6477 Bsp143I 1382 1714 2221 6253 6406 6502 6936 Bsp1407I 1021 BspPI 1382 2221 6252 6253 Bsu15I 2527 6882 BtgI 2763 6617 BtsI 6088 BveI 1113 2258 6268 CaiI 2187 CfrI 5080 6036 6293 Cfr9I 6247 Cfr10I 5613 Cfr13I 5724 5914 5938 6396 CseI 526 2164 2479 3237 4083 5158 6687 DpnI 1382 1714 2221 6253 6406 6502 6936 DraI 189 472 4622 6784 7074 Eam1104I 4074 6391 EciI 1962 6603 Ecl136II 6237 Eco24I 5643 6237 Eco47I 5914 Eco47III * 2710 3039 Eco57MI 6493 6899 Eco81I 6508 Eco88I 5825 6247 Eco105I 1268 EcoRI 6231 EcoRII 1014 1966 5941 5998 6137 6328 6455 EheI 6001 Esp3I 5971 FalI 2486 FaqI 5090 6529 FspBI 3827 5565 6259 6861 6979 GsuI 6493 6899 Hin1I 6001 Hin4I 252 442 1073 2845 5152 HincII 6264 HindIII 6282 Hpy8I 619 1022 2133 2717 4743 5713 5768 5785 5912 6264 6776 Hpy99I 610 1950 3634 4143 6297 6310 6525 6629 KpnI 6243 LweI 25 388 1354 3979 4850 6546 6559 MbiI 4569 5431 5572 6185 MboI 1382 1714 2221 6253 6406 6502 6936 MboII 781 2218 3912 4075 4271 4937 5255 5587 6391 6504 6805 MlsI 5080 MmeI 300 5441 5762 6627 MslI 2796 6996 MspA1I 1631 5960 6053 6375 MvaI 1014 1966 5941 5998 6137 6328 6455 Mva1269I 1746 NdeI 2723 3803 6846 NmuCI 1377 1775 2543 2621 2744 5111 5541 6314 NsbI 6425 PacI 4132 PaeI 6276 PagI 1299 PdiI 5613 PdmI 357 2646 PpiI 5766 PscI 195 3618 3717 PsiI 5356 5844 Psp1406I 4634 6773 PsuI 2220 6252 6935 PstI 6270 PvuI 6405 PvuII 5960 6053 6375 SacI 6237 SalI 6264 SchI 2011 2845 4072 5329 5766 5788 6262 6904 SdaI 6269 SduI 2088 4743 5465 5643 6237 SmaI 6247 SmiI 6783 SmlI 868 2005 2099 2612 5370 SmuI 1385 1984 3324 5430 5517 5571 6027 6084 6398 7131 SspI 499 2660 5023 5213 6767 6788 TaqI 336 1127 1508 1949 2528 3455 3694 4665 5683 6235 6265 6883 TauI 1394 2285 2288 2312 2357 5500 5514 TatI 1021 1164 1768 2132 4189 TseI 932 1367 2521 3132 4871 5536 5923 6052 6355 6428 TspGWI 750 1159 2654 2762 2876 4352 6619 TstI 6618 VspI 4131 4135 4238 4628 6046 6105 6966 XapI 1499 1674 2182 2728 3789 3900 4060 6231 6744 6755 7097 XbaI 6258 XceI 195 3531 3618 3717 6276 6856 XmiI 6264
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Updated liepos 16, 2007 09:25
