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GenBank/EMBL accession numbers J02459, M17233, M24325, V00636, X00906.
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
Enzymes which do not cut lambda DNA:
BcuI, FseI, NotI, PacI, SfiI, SgfI, SmiI, SrfI, TaqII.
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AarI 554 10835 13941 14383 16164 16194 16233 21242 29897 36293 37003 38481 AasI 5116 9104 11090 AatII 5105 9394 11243 14974 29036 40806 41113 42247 45563 45592 Acc65I 17053 18556 AdeI 2954 5613 6635 8999 14477 30365 31909 41479 47312 48434 AloI 266 7286 12056 21639 24654 36718 42867 Alw44I 5619 21798 27173 40216 ApaI 10086 BaeI 694 7665 13267 15775 16271 18732 19109 21779 43976 48385 BamHI 5505 22346 27972 34499 41732 BbvCI 8012 18147 18465 30916 31222 31836 35813 BclI 8844 9361 13820 32729 37352 43682 46366 47942 BfiI 7054 11608 25691 30332 BglII 415 22425 35711 38103 38754 38814 BoxI 8920 9394 13512 15412 36925 37889 48152 BplI 41036 Bpu1102I 10297 10682 11661 16518 20744 39450 BseSI 5619 5664 10086 11414 13039 14897 21798 27173 32330 40216 Bsp68I 4590 28050 31703 32407 41808 Bsp119I 18048 25884 27980 29150 30396 34331 42637 Bsp120I 10086 Bsp1407I 5220 6142 15855 29392 32496 BspTI 6540 12618 42630 BssSI 20356 25572 27956 29425 34430 35219 42416 42737 Bst1107I 15260 18834 19473 Cfr9I 19397 31617 39888 Cfr42I 20320 20530 21606 40386 CpoI 3800 6041 13983 19288 22242 Eam1105I 6398 11238 12477 12915 16588 18544 23460 30467 44669 Ecl136II 24772 25877 Eco24I 581 10086 19763 21570 24772 25877 39453 Eco31I 11424 42715 Eco47III 20995 37057 Eco52I 19944 36654 Eco72I 26529 41482 42362 Eco81I 26717 34318 Eco88I 4720 19397 20999 27887 31617 33498 38214 39888 Eco105I 12188 Eco130I 19329 21211 23901 24322 24396 27868 28793 35016 36505 44248 Eco147I 12434 31478 32997 39992 40596 40614 EcoO109I 2815 28797 48473 EcoRI 21226 26104 31747 39168 44972 EheI 45679 FspAI 21804 21825 HindIII 23130 25157 27479 36895 37459 37584 44141 KpnI 17053 18556 MluI 458 5548 15372 17791 19996 20952 22220 MssI 8459 16293 MunI 22687 22715 23054 25863 35764 37186 38332 47880 NcoI 19329 23901 27868 44248 NdeI 27630 29883 33679 36112 36668 38357 40131 NheI 34679 PaeI 2212 12002 23942 24371 27374 39418 PagI 889 4650 4989 10249 18275 28860 31608 40642 PauI 3522 4126 5627 14815 16649 28008 PdiI 20040 Pfl23II 19323 PpiI 7286 12057 17737 28950 36718 PscI 628 39395 PsiI 2285 9011 18941 19573 22014 24667 25472 27746 29498 29656 33236 37756 Psp5II 2815 28797 48473 Psp1406I 13529 16290 22580 22595 24642 43392 43488 PsrI 1697 20889 33098 PsyI 11202 36120 PvuI 11933 26254 35787 SacI 24772 25877 SalI 32745 33244 SanDI 28797 SapI 2397 6489 8702 10370 13286 24769 27234 34327 34800 47712 ScaI 16421 18684 25685 27263 32802 SdaI 2555 2819 11834 19832 37000 SexAI 22264 31009 32838 40497 44408 SgrAI 7064 8680 12878 15653 16974 31824 SgsI 3521 16648 SmaI 19397 31617 39888 XagI 13509 21292 22377 25174 25223 35521 38268 41842 47213 XbaI 24508 XcmI 958 4770 5065 5874 9193 9460 9490 14891 19329 23685 32530 36165 XhoI 33498 XmaJI 24322 24396 XmiI 2190 15260 18834 19473 31301 32745 33244 40201 42921
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Updated liepos 25, 2008 10:07
