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GenBank/EMBL accession numbers V01128, J02482, M10348, M10379, M10714, M10749, M10750, M10866, M10867, M24859.
Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in PhiX174 DNA for the following enzymes:
AarI, Acc65I, AloI, ApaI, BamHI, BclI, BcuI, BfiI, BglI, BglII, BplI, Bpu1102I, BsaXI, BsgI, BshTI, Bsp119I, Bsp120I, Bsp143I, Bsp1407I, BspPI, Bst1107I, Bsu15I, BtrI, CaiI, Cfr9I, Cfr10I, CpoI, DpnI, Ecl136II, Eco24I, Eco31I, Eco32I, Eco47III, Eco52I, Eco57I, Eco72I, Eco81I, Eco91I, Eco105I, Eco130I, EcoO109I, EcoRI, Esp3I, FseI, FspAI, HindIII, KpnI, Kpn2I, MboI, MlsI, Mph1103I, MssI, NcoI, NdeI, NheI, NotI, PacI, PaeI, PdiI, PpiI, PscI, Psp5II, PsuI, PsyI, PvuI, PvuII, SacI, SalI, SanDI, ScaI, SdaI, SfiI, SgfI, SgrAI, SgsI, SmaI, SmiI, SmuI, SrfI, TaqII, TatI, TstI, XagI, XbaI, XceI, XcmI, XmaJI.
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 5171 AatII 2782 AdeI 5183 AflIII 221 2146 Alw21I 538 4290 4779 Alw26I 673 1529 2903 3441 Alw44I 4779 BaeI 2581 BbvCI 2039 2341 3699 BccI 192 1048 1129 1262 1492 1751 2577 2743 3885 4118 4560 4897 BceA1I 462 627 666 895 1136 1414 3210 3642 4204 4759 5228 BcgI 967 5326 BcnI 2800 BfmI 1191 3061 4318 4801 5293 5382 BfuI 588 2580 3275 5373 Bme1390I 881 2800 3500 BoxI 1694 BpiI 2679 4367 4762 Bpu10I 1361 1835 2039 2341 2379 3306 3699 BpuEI 987 3631 BsaAI 826 4871 BsaWI 728 1102 3018 BseDI 880 1415 1434 2859 4681 5182 BseGI 228 648 1261 1419 1996 4265 4463 5152 BseJI 341 2716 BseMI 632 1161 2566 4539 BseMII 350 1527 1533 1836 2040 2342 3307 3700 4252 4738 BseNI 272 1016 1301 1777 2176 2185 3404 4850 4984 BseRI 232 1278 BseSI 4779 BseYI 5210 5279 Bsh1285I 4601 BshNI 1019 2478 2976 Bsp68I 2260 4424 BspLI 978 1019 2119 2478 2688 2976 BspTI 2914 4413 BssSI 535 3280 BsuRI 434 668 978 1172 1775 3128 4206 4487 4758 4876 4948 BstAPI 892 4781 BstXI 1777 2739 4984 BtgI 1415 2859 5182 BtsI 2203 BveI 3599 4052 5380 CfrI 4205 4757 Cfr13I 978 5042 Cfr42I 2859 Csp6I 414 571 768 906 1431 1903 2150 2795 3187 4747 4836 DraI 327 1406 Eam1104I 3746 4143 Eam1105I 1760 EciI 125 1876 Eco47I 5042 Eco57MI 2465 2905 4482 Eco88I 162 Eco147I 4486 EcoRII 881 3500 EheI 1019 2976 FalI 3172 FaqI 1214 4339 FspBI 3136 3907 5061 GsuI 2465 2905 4482 Hin1I 717 1019 1133 2782 2976 3363 5225 Hin4I 842 1072 1760 2233 2895 3339 4146 4236 5324 HpaII 729 1103 2800 3019 3367 HphI 1235 2012 2089 2132 2923 3033 3072 4188 4965 Hpy99I 543 719 1166 1767 4830 5219 5224 5300 KspAI 28 1292 5022 LweI 358 1420 1466 1733 2638 3075 3339 3431 3521 3795 4129 4956 MbiI 530 MboII 480 804 1661 2473 2562 2680 2683 3747 4143 4367 4763 MluI 221 2146 MmeI 225 2691 3237 5197 5376 MslI 1226 2165 2571 2740 2912 4554 4767 MspA1I 739 2339 2859 3259 3735 MspI 729 1103 2800 3019 3367 MunI 3939 MvaI 881 3500 Mva1269I 2570 3025 3463 4535 NmuCI 288 1224 2231 2773 3789 4009 4642 5322 NsbI 155 OliI 2912 PagI 388 1254 2271 PauI 5348 PdmI 3857 4143 5117 PfeI 204 310 1897 2015 2264 2877 3377 3731 4148 4701 5128 Pfl23II 413 2794 PsiI 2304 Psp1406I 864 2514 2809 PsrI 405 858 PstI 5382 RsaI 414 571 768 906 1431 1903 2150 2795 3187 4747 4836 SapI 3745 SchI 53 286 352 392 1118 1184 2677 3443 3543 3683 SduI 538 4290 4779 SexAI 3499 SmlI 162 987 2914 3631 4413 SspI 1007 TaqI 56 110 143 163 490 721 1125 4039 4180 5355 TspGWI 411 699 1149 1482 2004 2189 5184 TspRI 780 782 1239 1241 2046 2048 2175 2177 2202 2204 3789 3791 Van91I 3960 4095 VspI 711 4308 XapI 139 693 1983 2222 2324 2650 5340 XhoI 162 XmiI 1193 3545
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Updated liepos 16, 2007 09:25
