LOCUS SYNPUC18CV 2686 bp DNA circular SYN 05-MAR-1993 DEFINITION pUC18c cloning vector (beta-galactosidase mRNA on complementary strand). ACCESSION L09136 NID g209210 VERSION L09136.1 GI:209210 KEYWORDS beta-galactosidase; cloning vector. SOURCE Synthetic construct DNA. ORGANISM synthetic construct artificial sequence. REFERENCE 1 (bases 1 to 2686) AUTHORS Gilbert,W. TITLE Obtained from VecBase 3.0 JOURNAL Unpublished (1991) COMMENT These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. pUC18c - Cloning vector (beta-galactosidase mRNA on complementary strand) ENTRY PUC18C #TYPE DNA CIRCULAR TITLE pUC18c - Cloning vector (beta-galactosidase mRNA on complementary strand) DATE 02-APR-1986 #sequence 16-DEC-1986 ACCESSION VB0032 SOURCE artificial COLLECTION ATCC 37253 REFERENCE #number 1 #authors Norrander J., Kempe T., Messing J. #journal Gene (1983) 26: 101-106 REFERENCE #number 2 #authors Pouwels P.H., Enger-Valk B.E., Brammar W.J. #book Cloning Vectors, Elsvier 1985 and supplements #comment vector I-A-iv-20 COMMENT Assembled from pUC19c and M13mp18 by F. Pfeiffer, MPI, Martinsried Revised 16-DEC-1986 by F. Pfeiffer: 1062/3 'AT' to 'TA' to match revised sequence of PBR322 The beta-galactosidase mRNA sequence including the multiple cloning site of M13mp18 is on the strand complementary to that shown. KEYWORDS CROSSREFERENCE #complement VecBase(3):pUC18 PARENT Features of pUC18c (2686 bp) residue source 1- 137 2074-2210 pBR322 138- 237 2252-2351 pBR322 238- 396 1461-1303 (c) Lac-Operon 399- 455 57- 1 (c) polylinker of M13mp18 456- 682 1297-1071 (c) Lac-Operon 683-2686 2352-4355 pBR322 Conflict (cfl) and Mutations (mut): pUC18c source mut 1942 A G 3611 pBR322 mut 2243 T C 3912 pBR322 FEATURE 1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-PstI-SphI-HindIII SELECTION #resistance Ap #indicator beta-galactosidase SUMMARY pUC18c #length 2686 #checksum 3662. FEATURES Location/Qualifiers source 1..2686 /organism="synthetic construct" /db_xref="taxon:32630" BASE COUNT 666 a 677 c 684 g 659 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg 421 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct 481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1621 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1681 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1741 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 1801 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1861 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1921 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 1981 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2041 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2101 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2161 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2221 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 2281 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2341 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2401 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2461 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 2521 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 2581 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 2641 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc //