LOCUS PTZ19UCS 2862 bp DNA SYN 24-SEP-1997 DEFINITION Phagemid cloning vector pTZ19U, complete sequence. ACCESSION Y14836 NID g2440159 VERSION Y14836.1 GI:2440159 KEYWORDS beta-galactosidase; beta-lactamase; bla gene; lac repressor; lacI gene; lacz gene; T7 promoter. SOURCE Phagemid cloning vector pTZ19U. ORGANISM Phagemid cloning vector pTZ19U artificial sequence; vectors. REFERENCE 1 (bases 1 to 2862) AUTHORS Markauskas,A. TITLE Direct Submission JOURNAL Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, Vilnius 2028, LITHUANIA REFERENCE 2 (bases 1 to 2862) AUTHORS Markausakas,A., Lubiene,J. and Lubys,A. TITLE Revised complete sequence of phagemid pTZ19U JOURNAL Unpublished FEATURES Location/Qualifiers source 1..2862 /organism="Phagemid cloning vector pTZ19U" /db_xref="taxon:66197" /lab_host="E.coli" misc_feature 1..456 /note="intergenic region of phage f1" misc_feature complement(163) /note="start of (-) strand DNA synthesis" misc_feature 188 /note="start of (+) strand DNA synthesis" gene complement(239..715) /gene="lacZ" CDS complement(239..715) /gene="lacZ" /note="N-terminal fragment" /codon_start=1 /transl_table=11 /product="beta-galactosidase" /protein_id="CAA75108.1" /db_xref="PID:e350493" /db_xref="PID:g2440160" /db_xref="GI:2440160" /translation="MTMITPSSNTTHYRESLHACRSTLEDPRVPSSNSLAVVLQRRDW ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVT RSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD" promoter complement(673..692) /gene="lacZ" /note="T7" protein_bind complement(733..753) /note="lac repressor" /bound_moiety="lacI" protein_bind complement(800..837) /bound_moiety="CAP" misc_feature 1098..1712 /note="replicon of pMB1" rep_origin complement(1112) gene complement(1872..2732) /gene="bla" CDS complement(1872..2732) /gene="bla" /function="confers resistance to ampicilin" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="CAA75109.1" /db_xref="PID:e350494" /db_xref="PID:g2440161" /db_xref="GI:2440161" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" mat_peptide complement(1875..2663) /gene="bla" /product="beta-lactamase" sig_peptide complement(2664..2732) /gene="bla" BASE COUNT 731 a 709 c 721 g 701 t ORIGIN 1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca 481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 601 aaacgacggc cagtgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg 661 catgcaagct ttccctatag tgagtcgtat tagagcttgg cgtaatcatg gtcatagctg 721 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 781 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 841 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 901 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 1021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 1081 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 1141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 1201 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 1261 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 1321 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 1381 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 1441 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 1501 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 1561 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 1621 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 1681 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 1741 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 1801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 1861 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 1921 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 1981 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 2041 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 2101 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 2161 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 2221 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 2281 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 2341 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 2401 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 2461 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 2521 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 2581 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 2641 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 2701 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 2761 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 2821 caaatagggg ttccgcgcac atttccccga aaagtgccac ct //