LOCUS SYNM13M19V 7250 bp DNA circular SYN 08-FEB-1994 DEFINITION M13mp19 phage cloning vector. ACCESSION L08821 NID g310749 VERSION L08821.1 GI:310749 KEYWORDS . SOURCE Synthetic construct DNA. ORGANISM synthetic construct artificial sequence. REFERENCE 1 (bases 1 to 7250) AUTHORS Gilbert,W. TITLE Obtained from VecBase 3.0 JOURNAL Unpublished (1991) COMMENT These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. M13mp19 - Phage cloning vector ENTRY M13MP19 #TYPE DNA CIRCULAR TITLE M13mp19 - Phage cloning vector DATE 27-MAR-1986 #sequence 02-APR-1986 ACCESSION VB0019 SOURCE artificial REFERENCE #number 1 #authors Norrander J., Kempe T., Messing J. #journal Gene (1983) 26: 101-106 REFERENCE #number 2 #authors Pouwels P.H., Enger-Valk B.E., Brammar W.J. #book Cloning Vectors, Elsvier 1985 and supplements #comment vector I-A-v-1 COMMENT Assembled from M13mp18 by F. Pfeiffer, MPI, Martinsried KEYWORDS CROSSREFERENCE #parent VecBase(3):M13mp11 #parent GenBank(50):M13, GenBank(50):EcoLac, VecSource(3):bGal19 #brother VecBase(3):M13mp18 #offspring VecBase(3):pUC19, VecBase(3):pUC19c, VecBase(3):PhgeScSK PARENT Features of M13mp19 (7250 bp) residue source 1-5868 1-5868 phage M13 5869-6231 936-1298 Lac-Operon 6234-6290 1- 57 M13mp19/pUC19-Polylinker 6291-6711 1304-1724 Lac-Operon 6712-7250 5869-6407 phage M13 Conflict (cfl) and Mutations (mut): M13mp19 source mut 3 T C 3 phage M13 mut 2220 A G 2220 phage M13 cfl 5977 G A 1044 Lac cfl 6522 G T 1535 Lac mut 6938 T C 6095 phage M13 mut 6968 T G 6125 phage M13 Position 898 is TT in Messing's sequence, but TTT in M13 wildtype. It was changed to TTT to restore the reading frame of gene V. M13mp19 does not contain amber mutations in gene I and gene II. FEATURE POLYLINKER HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION #indicator beta-galactosidase SUMMARY M13mp19 #length 7250 #checksum 8102. FEATURES Location/Qualifiers source 1..7250 /organism="synthetic construct" /db_xref="taxon:32630" BASE COUNT 1768 a 1540 c 1531 g 2411 t ORIGIN 1 aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta 181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca 241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg 301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag 361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt 421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct 541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt 601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt 661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg 721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca 841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt 901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg 961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc 1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc 1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat 1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt 1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta 1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct 1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga 1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta 1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa 1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt 1561 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc 1621 tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca 1681 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt 1741 ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca 1801 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt 1861 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct 1921 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa 1981 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt 2041 cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact 2101 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg 2161 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa 2221 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat 2281 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt 2341 ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt 2401 gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 2461 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 2521 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact 2581 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct 2641 ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct 2701 tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta 2761 ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg 2821 tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt 2881 tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc 2941 ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg 3001 ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact 3061 ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc 3121 tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg 3181 attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg 3241 ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat 3301 cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt 3361 cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat 3421 tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat 3481 acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt 3541 aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg 3601 cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct 3661 tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat 3721 gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat 3781 actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat 3841 tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta 3901 aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt 3961 tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg 4021 gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct 4081 cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat 4141 agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc 4201 attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt 4261 tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt 4321 tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg 4381 tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc 4441 tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta 4501 taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga 4561 tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac 4621 ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa 4681 gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt 4741 tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc 4801 aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga 4861 tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg 4921 cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt 4981 agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg 5041 tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat 5101 tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg 5161 tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt 5221 tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat 5281 tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact 5341 cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa 5401 aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt 5461 atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg 5521 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 5581 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 5641 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 5701 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 5761 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 5821 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa 5881 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg 5941 ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct 6001 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 6061 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 6121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 6181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 6241 catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc actggccgtc 6301 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 6361 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 6421 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg 6481 ccggaaagct ggctggagtg cgatcttcct gaggccgata cggtcgtcgt cccctcaaac 6541 tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc 6601 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt 6661 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt tcctattggt 6721 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta 6781 caatttaaat atttgcttat acaatcttcc tgtttttggg gcttttctga ttatcaaccg 6841 gggtacatat gattgacatg ctagttttac gattaccgtt catcgattct cttgtttgct 6901 ccagactctc aggcaatgac ctgatagcct ttgtagatct ctcaaaaata gctaccctct 6961 ccggcattaa tttatcagct agaacggttg aatatcatat tgatggtgat ttgactgtct 7021 ccggcctttc tcaccctttt gaatctttac ctacacatta ctcaggcatt gcatttaaaa 7081 tatatgaggg ttctaaaaat ttttatcctt gcgttgaaat aaaggcttct cccgcaaaag 7141 tattacaggg tcataatgtt tttggtacaa ccgatttagc tttatgctct gaggctttat 7201 tgcttaattt tgctaattct ttgccttgcc tgtatgattt attggatgtt //