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pUC57: description & restriction map

Related Documents: GenBank/EMBL  accession number Y14837. Restriction sites Sequence Ordering information

Additional Information:

• CAP protein binding site - 615-578 (compl. strand);

• mRNA (LacZ) starts at nt position 531 (compl. strand);
• lac repressor binding site -531-511 (compl. strand).

Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3'-ends, that are resistant to E.coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact position of genetic elements is shown on the map (termination codons included). DNA replication initiates at position 890 (+/- 1) and proceeds in indicated direction. The bla gene nucleotides 2510-2442 (compl. strand) code for a single peptide.
The map shows enzymes that cut pUC57 DNA once. Enzymes produced by Fermentas are shown in blue. The coordinates refer to the position of the first nucleotide in each recognition sequence.

Enzymes which cut pUC57 DNA once:
AatII 2641, Acc65I 408, AflIII 830, AlfI 455, ApaI 442, BamHI 435, BcgI 2239, BsaXI 683, BseYI 1134, Bsp68I 414, Bsp120I 442, BstAPI 179, CaiI 1241, Cfr9I 439, Cfr10I 1803, Eam1105I 1718, Ecl136II 402, Eco31I 1790, Eco32I 429, Eco88I 439, Eco147I 459, EcoO109I 2698, EcoRI 396, EheI 235, GsuI 1808, HincII 448, HindIII 471, KpnI 408, Mph1103I 420, Mva1269I 418, NdeI 183, PaeI 465, PdmI 2318, PfoI 46, PscI 830, PstI 453, SacI 402, SalI 448, SapI 707, ScaI 2201, SmaI 439, SspI 2525, XapI 396, XbaI 425, XmiI 448.

Multiple Cloning Sites

pUC57

catalog@fermentas.com

Updated liepos 25, 2008 10:13