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pTZ57R: description & restriction map

Related Documents: Restriction sites Sequence Not available from Fermentas

Additional Information:

• CAP protein binding site - 861-824 (compl. strand);

• mRNA (LacZ) starts at nt position 777 (compl. strand);
• lac repressor (LacI) binding site - 777-757 (compl. strand);
• f1 packaging signal - 3-94.

Plasmid pTZ57R, 2886 bp in length, is a derivative of pTZ19R. pTZ57R MCS contains 6 restriction sites with protruding 3'-ends, that are resistant to E.coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact position of genetic elements is shown on the map (termination codons included). DNA replication initiates at position 1136 (+/- 1) and proceeds in indicated direction. The bla gene nucleotides 2688-2756 (complementary strand) code for a signal peptide.

Reference

1. Mead, D.A., Szczesna-Skorupa, E. and Kemper, B., Single-stranded DNA áblue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering, Protein Eng., 1, 67-74, 1986.

Enzymes which cut pTZ57R DNA once:
Acc65I 627, AdeI 230, AflIII 1076, AlfI 674, AloI 279, ApaI 661, BamHI 654, BcgI 2485, BsaAI 230, BseYI 1380, Bsp68I 633, Bsp120I 661, CaiI 1487, Cfr9I 658, Eam1105I 1964, Ecl136II 621, Eco31I 2036, Eco32I 648, Eco88I 648, Eco147I 678, EcoRI 615, GsuI 2054, Hin1I 2505, HincII 667, HindIII 690, KpnI 627, Mph1103I 639, Mva1269I 637, PaeI 684, PdiI 127, PdmI 2564, PscI 1076, PsiI 358, PstI 672, SacI 621, SalI 667, SapI 953, ScaI 2447, SmaI 658, TatI 2447, XbaI 644, XmiI 667.

Multiple Cloning Sites

pTZ57R

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Updated lapkričio 08, 2006 14:20