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Phage Lambda: description & restriction map

Related Documents: GenBank/EMBL accession numbers J02459, M17233, M24325, V00636, X00906. Restriction sites Sequence Ordering information

Lambda is a temperate Escherichia coli bacteriophage. Virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5'-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis and virion proteins are expressed. The chromosome replicates and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by recombination.

Phage lambda DNA is a common substrate for restriction enzymes and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is very often in use. The DNA sequence used to construct a phage lambda restriction map includes these mutations. The map shows enzymes that cut lambda DNA once. Enzymes produced by Fermentas are shown in blue.

Enzymes which cut lambda DNA once:

ApaI 10086,  BplI 41036,  Bsp120I 10086,  Eco105I 12188,  EheI 45679,  NheI 34679,  PdiI 20040,  Pfl23II 19323,  SanDI 28797,  XbaI 24508,  XhoI 33498.

 

References

1. Daniels, D.L., Schroeder, J.L., Szybalski, W., Sanger, F. and Blattner, F.R., Appendix I: A molecular map of coliphage lambda. (in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. and Weisberg,R.A. (Eds.), LAMBDA II: 469-517; Cold Spring Harbor Laboratory, Cold Spring Harbor, 1983.

2. Daniels, D.L., Schroeder, J.L., Szybalski, W., Sanger, F., Coulson, A.R., Hong, G.F., Hill, D.F., Petersen, G.B. and Blattner, F.R., Appendix II: Complete annotated lambda sequence. (in) Hendrix, R.W., Roberts, J.W., Stahl, F.W. and Weisberg, R.A. (Eds.), LAMBDA II: 519-674; Cold Spring Harbor Laboratory, Cold Spring Harbor, 1983.

3. Sanger, F., Coulson, A.R., Hong, G.F., Hill, D.F. and Petersen, G.B., Nucleotide sequence of bacteriophage lambda DNA, J. Mol. Biol. ,162, 729-773, 1982.

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Updated lapkričio 08, 2006 14:13