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Purification of Genomic DNA from Mouse Tail
(with Proteinase K)

1. Place a 0.5-1 cm sample of mouse tail into a 1.5 ml microcentrifuge tube.
2. Add 500 µl of lysis buffer (50 mM KCl, 50 mM Tris-HCl (pH 8.0), 2.5 mM EDTA, 0.45% NP-40, 0.45% Tween-20).
3. Add 2.5 µl of Proteinase K, 20 mg/ml and mix briefly.
4. Incubate overnight at 55°C.
   Optional. Incubate for an additional 1 hour at 65°C.
5. Vortex and spin 10 seconds at 13,000 rpm to collect cell debris.
6. Use 1 µl from the top part of the supernatant per 50 µl of PCR mix.

Ordering Information

Proteinase K RNase A/T1 Mix Water, nuclease-free

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Updated balandžio 02, 2008 08:12