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Protocol for the Removal of Template DNA Following in vitro Synthesis of RNA
(with DNase I, RNase-free)

1. Add 2 u of DNase I, RNase-free per 1 µg of template DNA directly to a transcription reaction mixture. In some cases, the amount of enzyme required to completely digest a given amount of DNA must be determined empirically.
2. Incubate at 37°C for 15 minutes. 
3. Inactivate DNase I, RNase-free by phenol/chloroform extraction.

References

1. Sambrook, J., Russell, D.W., Molecular Cloning: A Laboratory Manual, the third edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2001. 

Ordering Information

DNase I, RNase-free SP6 RNA Polymerase T3 RNA Polymerase T7 RNA Polymerase M-MuLV Reverse Transcriptase RevertAid™ H Minus M-MuLV Reverse Transcriptase RevertAid™ M-MuLV Reverse Transcriptase DNA Polymerase I, E.coli RiboLock™ RNase Inhibitor First Strand cDNA Synthesis Kit ReverAid™ H Minus First Strand cDNA Synthesis Kit ReverAid™ First Strand cDNA Synthesis Kit 0.5 M EDTA, pH 8.0 Water, nuclease-free DEPC-treated Water

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Updated sausio 31, 2008 15:20