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Conditions for Quality Control Assays (QCA)

Enzyme	Amount of  enzyme/reaction	Incubation temp.,  °C	Incubation time,  hour	QCA  performed
TrueStart™ Taq DNA Polymerase	10 units	37, 70	4	dsENDO, EXO II,RNase
Pfu DNA Polymerase (native)	10 units	72	4	dsENDO, EXO II
Pfu DNA Polymerase (recombinant)	10 units	37, 72	4	dsENDO, EXO II
Taq DNA Polymerase (recombinant)	10 units	37, 70	4	dsENDO, EXO II, RNase
Taq DNA Polymerase (native, without BSA)	10 units	70	4	dsENDO, EXO II, RNase
Taq DNA Polymerase (native, without BSA)	10 units	70	4	dsENDO, EXO II, RNase
DNA Polymerase I, E.coli	20 units	37	4	dsENDO
Klenow Fragment	20 units	37	4	dsENDO
Klenow Fragment, exo-	20 units	37	4	dsENDO, EXO I, LO
phi29 DNA Polymerase	100 units	30	4	ds-, ssENDO
T4 DNA Polymerase	10 units	37	4	ds-, ssENDO
T7 DNA Polymerase	10 units	37	4	dsENDO
Terminal Deoxynucleotidyl Transferase	60 units	37	4	dsENDO, LO, RNase
M-MuLV Reverse Transcriptase	200 units	37	4	dsENDO, LO, RNase
RevertAid™ M-MuLV Reverse Transcriptase	2000 units	37	4	dsENDO, LO, RNase
RevertAid™ H Minus M-MuLV Reverse Transcriptase	2000 units	37	4	dsENDO, LO, RNase
T3 RNA Polymerase	200 units	37	1	dsENDO, EXO I, RNase
T7 RNA Polymerase	200 units	37	1	dsENDO, EXO I, RNase
SP6 RNA Polymerase	200 units	37	1	dsENDO, EXO I, RNase
RiboLock™ RNase Inhibitor	200 units	37	4	dsENDO, LO, RNase
DNase I, RNase-free	10 units	37	4	RNase, Protease
Endonuclease IV, E.coli	20 units	37	1	dsENDO, EXO I, RNase
Endonuclease V, T.maritima	25 units	37	4	dsENDO, EXO I, LO, RNase
Exonuclease I, E.coli	100 units	37	16	ds-, ssENDO, EXO II, RNase
Exonuclease III, E.coli	25 units	37	4	dsENDO
Lambda Exonuclease	100 units	37	4	dsENDO
RNase A, DNase and protease-free	5 µg	37	18	dsENDO, LO
	25 µg	37	18	Protease
RNase T1	1000 units	37	18	dsENDO, LO
	10,000 units	37	18	Protease
RNases A/T1 Mix	2 µl	37	18	dsENDO, LO
	10 µl	37	18	Protease
RNase H, E.coli	10 units	37	1	dsENDO, EXO I, RNase
RNase I, E.coli	80 units	37	4	dsENDO, EXO I, LO
T4 DNA Ligase	200 units	37	4	dsENDO, LO, B/W, RNase
T4 RNA Ligase	50 units	37	4	dsENDO, LO, RNase
Calf Intestine Alkaline Phosphatase (CIAP)	50 units	37	1	dsENDO, EXO I, B/W, RNase
Shrimp Alkaline Phosphatase (SAP)	10 units	37	1	dsENDO, EXO I, B/W, RNase
T4 Polynucleotide Kinase	50 units	37	4	dsENDO, EXO I, RNase
Agarase	5 units	37	4	dsENDO, LO, B/W, RNase
Proteinase K	0.5 units	37	4	dsENDO, EXO I, RNase
Pyrophosphatase, Inorganic (from yeast)	1 unit	37	24	dsENDO, EXO I, LO, RNase
Uracil-DNA Glycosylase	50 units	37	4	dsENDO, LO, RNase

catalog@fermentas.com

Updated sausio 31, 2008 15:25