DNA/RNA Modifying Enzymes

• Products
• Certificates of Analysis
• MSDS
• Support
• Application Protocols:
  • cDNA Synthesis
  • Cloning
  • Labeling
    • DNA
    • RNA
  • PCR
  • Purification
  • Sequencing
  • Transcription

2008-2009 Catalog & Product Application Guide
DNA/RNA MODIFYING ENZYMES, 1.25 MB

 Support:

• Quality Guarantee & Quality Control Assays
• Storage and Shipping
• Conditions for Quality Control Assays (QCA)
• Activity of DNA/RNA Modifying Enzymes in Buffers for Restriction Enzymes, PCR and RT
• Description of Icons

 Application Protocols:

cDNA Synthesis

• First-strand cDNA Synthesis with AMV Reverse Transcriptase new
• First-strand cDNA Synthesis with M-MuLV Reverse Transcriptase
• First-strand cDNA Synthesis with RevertAid™ M-MuLV Reverse Transcriptase
• First-strand cDNA Synthesis with RevertAid™ H Minus M-MuLV Reverse Transcriptase
• Preparation of DNA-free RNA Prior to RT-PCR with DNase I, RNase-free
• Second Strand cDNA Synthesis with DNA Polymerase I, E.coli and RNase H, E.coli

Cloning

• Fill-in Recessed 3'-termini of Double-stranded DNA with Klenow Fragment
• Fill-in Recessed 3'-termini of Double-stranded DNA with T7 DNA Polymerase
• Blunting DNA with 5'- or 3'-protruding Termini with T4 DNA Polymerase
• DNA Tailing with Terminal Deoxynucleotidyl Transferase
• DNA Insert Ligation into Vector DNA with T4 DNA Ligase
• Self-circularization of Linear DNA with T4 DNA Ligase
• Linker Ligation with T4 DNA Ligase
• Generation of Unidirectional Deletions in DNA Fragments (for 25 time points) with Exonuclease III
• Creation of Nested Deletions with Nb.Bpu10I
• Preparation of Covalently Closed, Double-stranded Linear DNA Molecules with Nb.Bpu10I
• Dephosphorylation of DNA 5'-termini with FastAP™ Thermosensitive Alkaline Phosphatase new
• Dephosphorylation of DNA 5'-termini with Shrimp Alkaline Phosphatase (SAP)
• Dephosphorylation of DNA 5'-termini with Calf Intestine Alkaline Phosphatase (CIAP)
• Fast Simultaneous Plasmid Vector Linearization and Dephosphorylation with FastAP™ Thermosensitive Alkaline Phosphatase new
• DNA Digestion and Subsequent Dephosphorylation with Shrimp Alkaline Phosphatase (SAP)

Labeling

DNA:

• DNA Labeling by Nick-translation with DNA Polymerase I, E.coli:
  • Nick-translation using Radioactive Nucleotides
  • Nick-translation using Biotin-11-dUTP
• Labeling Recessed 3'-termini of Double-stranded DNA with Klenow Fragment
• Fill-in Recessed 3'-termini of Double-stranded DNA with Klenow Fragment
• Fill-in Recessed 3'-termini of Double-stranded DNA with T7 DNA Polymerase
• Random-primed DNA Labeling with Klenow Fragment, exo-
• DNA Tailing with Terminal Deoxynucleotidyl Transferase
• Labeling DNA 3'-termini with Terminal Deoxynucleotidyl Transferase
• Labeling 5'-termini of DNA by:
  • Forward Reaction with T4 Polynucleotide Kinase
  • Exchange Reaction with T4 Polynucleotide Kinase
• Phosphorylation of 5'-termini of DNA or Oligonucleotide with T4 Polynucleotide Kinase
• Dephosphorylation of DNA 5'-termini with Shrimp Alkaline Phosphatase (SAP)
• Dephosphorylation of DNA 5'-termini with Calf Intestine Alkaline Phosphatase (CIAP)
• see also
  • Radioactive DNA Marker Labeling with T4 Polynucleotide Kinase, Klenow Fragment

RNA:

• Radioactive RiboRuler™ RNA Ladder Labeling with T4 Polynucleotide Kinase

PCR

• Protocol for PCR with Taq DNA Polymerase
• Protocol for PCR with Pfu DNA Polymerase
• Protocol for PCR with Hot Start Taq DNA Polymerase
• Protocol for PCR with TrueStart™ Taq DNA Polymerase
• First-strand cDNA Synthesis with M-MuLV Reverse Transcriptase
• First-strand cDNA Synthesis with RevertAid™ and RevertAid™ H Minus M-MuLV Reverse Transcriptases
• PCR Product Clean-up with Exonuclease I, E.coli and FastAP™ Thermosensitive Alkaline Phosphatase or Calf Intestine Alkaline Phosphatase (CIAP) or Shrimp Alkaline Phosphatase (SAP)
• Preparation of DNA-free RNA Prior to RT-PCR with DNase I, RNase-free

Purification

• DNA Purification after Enzymatic Reaction by Phenol/chloroform Extraction and Alcohol Precipitation
• Purification of Genomic DNA from Mouse Tail with Proteinase K
• Purification of DNA from Cultured Eukaryotic Cells with Proteinase K
• Isolation of Genomic DNA from Mammalian Cells with RNase A, DNase and protease-free
• Isolation of Plasmid DNA (a mini-preparation) with RNase A/T1 Mix
• The Recovery of DNA from Low Melting Point Agarose Gels with Agarase

Sequencing

• Elimination of Residual Oligonucleotides and dNTPs from PCR Mixes with Exonuclease I, E.coli
• Production of Single-stranded Circular DNA Molecules from Supercoiled Double-stranded Plasmids in vitro with Nb.Bpu10I

Transcription

• Synthesis of RNA with Sp6/T3/T7 RNA Polymerases
• Synthesis of High Specific Activity Radiolabeled RNA with Sp6/T3/T7 RNA Polymerases
• Removal of Template DNA Following in vitro Synthesis of RNA with DNase I, RNase-free

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