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Recommendations for Use

To prepare sterile growth media, the use of clean glassware is sufficient. Sterile glassware is not required.

1. Pour the pouch contents into a 500 ml glass bottle or flask.

2. Add 200 ml deionized or distilled water.
3. Heat the medium in a microwave oven on MEDIUM power setting until bubbles start to appear (2-3 minutes). Swirl gently to mix the preparation.
4. Reheat the medium for 30 seconds and swirl gently again. Do not overboil.
5. Repeat step 4 if necessary until the medium is completely dissolved.
6. Allow the medium to cool and pour plates (for solid media) or use directly (for liquid media).

For solid media, leaving plates in a sterile air flow cabinet with the lids slightly open allows for faster solidification of the media and prevention of water condensation on the plates.

Do not autoclave! 

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Updated gegužės 09, 2007 16:18