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S U P P O R T
 

Recommendations for Loading DNA Ladders & Markers

Note

ZipRuler™ Express DNA Ladders

Step 1:  mix gently
Step 2:  load the ladder 1 and the ladder 2 in two different wells (0.5-1 µl per 1 mm gel lane).

Recommendations:

Ordering Information

FastRuler™ DNA Ladders

2x500 µl is sufficient for 50-333 applications; 333 at 3 µl/lane.
For recommended loading amounts see.

For accurate quantification:

Vortex gently before use.
Do not heat before loading.

Ordering Information

MassRuler™ DNA Ladders, ready-to-use

2x500 µl is sufficient for 50-200 applications; 200 at 5 µl/lane.
For recommended loading amounts see.

For accurate quantification:

Vortex gently before use.
Do not heat before loading.

Ordering Information

O'RangeRuler™ DNA Ladders, ready-to-use

500 µl (25 µg) is sufficient for ~100 applications, 5 µl (0.25 µg)/lane.
Apply 1 µl (0.05 µg) of DNA ladder per 1 mm of an agarose or polyacrylamide gel lane width.
Do not heat before loading.

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Ready-to-use GeneRuler™, O'GeneRuler™ DNA Ladders & Conventional DNA Markers

500 µl (50 µg) is sufficient for ~100 applications, 5 µl (0.5 µg)/lane.
Apply 1 µl (0.1 µg) of ready-to-use DNA ladder/marker per 1 mm of an agarose gel lane width.
Apply 1-2 µl (0.1-0.2 µg) of ready-to-use DNA ladder/marker per 1 mm of a polyacrylamide gel lane width.

Ordering Information

GeneRuler™ DNA Ladders & Conventional Markers

Loading on an agarose gel:
50 µg (100 µl) is sufficient for ~100 applications, 0.5 µg (1 µl)/lane.
Use 0.1 µg (0.2 µl) of DNA ladder/marker per 1 mm of an agarose gel lane width.

  1. Prepare the following mixture for loading GeneRuler™ DNA ladders / conventional DNA markers:
    DNA ladder/marker 1 µl (0.5 µg)
    6X Loading Dye Solution 1 µl
    Water, nuclease-free up to 6 µl
  2. Vortex gently just prior to use.
  3. Heat lambda DNA marker at 65°C for 5 min and chill on ice for 3 min before use.
  4. Apply the prepared amount (6 µl) of DNA ladder/marker on a 5 mm lane of agarose gel.

Loading on a polyacrylamide gel (1-3):
50 µg (100 µl) is sufficient for ~50 applications, 1 µg (2 µl)/lane.
Use 0.2 µg (0.4 µl) of DNA ladder/marker per 1 mm of a polyacrylamide gel lane width.

  1. Prepare the following mixture for loading GeneRuler™ DNA ladders / conventional DNA markers:
    DNA ladder/marker 2 µl (1 µg)
    6X Loading Dye Solution 0.5 µl
    Water, nuclease-free up to 3 µl
  2. Vortex gently just prior to use.
  3. Apply the prepared amount (3 µl) of DNA ladder / marker on a 5 mm lane of polyacrylamide gel.

References

  1. Stellwagen, N.C., Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gels, Biochemistry, 22, 6186-6193, 1983.

  2. Lane, D., et al., Use of gel ratardation to analyze protein – nucleic acid interactions, Microbiological Reviews, 56, 509-528, 1992.
  3. Stellwagen, N.C., Conformational isomers of curved DNA molecules can be observed by polyacrylamide gel electrophoresis, Electrophoresis, 21, 2327-2334, 2000.
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DNA Markers for Genomic DNA Analysis

6 µg (30 µl) is sufficient for ~120 applications, 50 ng/lane.
Use ~6ng of DNA marker per 1 mm of an agarose gel lane width.

  1. Dilute 1 µl (0.2 µg) of DNA marker with 39 µl of nuclease-free water and mix.
  2. Prepare for loading:
    DNA marker mix 10 µl (50 ng)
    10X DNA Loading Dye 1.5 µl
    Water, nuclease-free up to 15 µl
  3. Vortex gently just prior to use.
  4. Heat lambda DNA marker at 65°C for 5 min and chill on ice for 3 min before use.
  5. Apply the prepared amount (15 µl) of DNA marker on a 8 mm lane of agarose gel.
Ordering Information

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Updated vasario 01, 2008 10:49