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Computer Generated Digests

The following restriction digests were calculated by applying empirical formula which approximates electrophoresis in 1.4% agarose gel:

y = -tanh(1.21*(log₁₀(x)-3.04))

x - DNA fragment length in base pairs;
y - relative distance

• Lambda DNA
• PhiX174 DNA
• M13mp18 DNA
• pBR322 DNA
• pUC18 DNA
• pUC57 DNA
• pTZ19R DNA
• pBluescript II KS(+) DNA
• pBluescript II SK(-) DNA
• pACYC177 DNA
• pACYC184 DNA

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Updated balandžio 06, 2006 04:07