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Collection of NoLimits™ DNA Fragments

NoLimits™ DNA fragments from 300 bp to 10 kb. 1.1% TopVision™ LE GQ Agarose, 1X TAE, 7 V/cm, 30 min.

Fermentas, an industry quality leader for DNA markers and ladders, is offering a unique opportunity to create the highest quality DNA ladder of desired range and composition from the collection of NoLimits™ DNA fragments (see table below). Our collection of 36 precisely-produced individual DNA fragments covers the 10-20,000 bp range. This collection is continuously expanded.
Our DNA size standards are not produced by PCR, like DNA standards from some other vendors. Fermentas custom DNA fragments are produced using specifically designed plasmids purified by a proprietary patent-pending technology. Plasmid DNA is digested with PureExtreme® restriction enzymes and each DNA fragment is chromatography-purified from the digestion mixture. As a result, we offer exceptionally pure, precise DNA fragments that are free of any truncated or degraded molecules. Our custom DNA fragments are the best choice for applications ranging from electrophoresis (both gel and capillary) to HPLC, and beyond.

Use NoLimits™ Custom DNA Ladders Service to order bulk quantities of custom DNA fragments, as well as custom DNA ladders according to your specifications.

Features

• Chromatography-purified, exceptionally pure individual DNA fragments.
• Collection of 36 fragments is continuously expanded.
• Precise DNA concentration allows mixing of the fragments to produce any ladder.
• Broad size range: 10-20,000 bp.
• Sharp peaks during capillary electrophoresis.
• Sharp bands during gel electrophoresis.

Applications

• Gel electrophoresis.
• Capillary electrophoresis.
• DNA quantification.
• HPLC analysis.
• Specific applications where the high purity and specific compositions of DNA size standard is needed.

We offer the following NoLimits™ DNA fragments:

Product name	Catalog #	Certificate of Analysis   (in pdft, ~80 KB)	Product resources (in pdf, ~40 KB)	Amount, µg
NoLimits™ 10 bp DNA Fragment	SM1391		MSDS	10
NoLimits™ 15 bp DNA Fragment	SM1381		MSDS	10
NoLimits™ 20 bp DNA Fragment	SM1401		MSDS	10
NoLimits™ 25 bp DNA Fragment	SM1761		MSDS	10
NoLimits™ 35 bp DNA Fragment	SM1411		MSDS	10
NoLimits™ 50 bp DNA Fragment	SM1421		MSDS	10
NoLimits™ 75 bp DNA Fragment	SM1431		MSDS	10
NoLimits™ 100 bp DNA Fragment	SM1441		MSDS	10
NoLimits™ 150 bp DNA Fragment	SM1601		MSDS	10
NoLimits™ 200 bp DNA Fragment	SM1611		MSDS	10
NoLimits™ 250 bp DNA Fragment	SM1451		MSDS	10
NoLimits™ 300 bp DNA Fragment	SM1621		MSDS	10
NoLimits™ 400 bp DNA Fragment	SM1631		MSDS	10
NoLimits™ 500 bp DNA Fragment	SM1641		MSDS	10
NoLimits™ 600 bp DNA Fragment	SM1461		MSDS	10
NoLimits™ 700 bp DNA Fragment	SM1651		MSDS	10
NoLimits™ 750 bp DNA Fragment	SM1471		MSDS	10
NoLimits™ 800 bp DNA Fragment	SM1481		MSDS	10
NoLimits™ 850 bp DNA Fragment	SM1661		MSDS	10
NoLimits™ 900 bp DNA Fragment	SM1491		MSDS	10
NoLimits™ 1000 bp DNA Fragment	SM1671		MSDS	10
NoLimits™ 1200 bp DNA Fragment	SM1681		MSDS	10
NoLimits™ 1500 bp DNA Fragment	SM1691		MSDS	10
NoLimits™ 2000 bp DNA Fragment	SM1701		MSDS	10
NoLimits™ 2500 bp DNA Fragment	SM1571		MSDS	10
NoLimits™ 3000 bp DNA Fragment	SM1711		MSDS	10
NoLimits™ 3500 bp DNA Fragment	SM1501		MSDS	10
NoLimits™ 4000 bp DNA Fragment	SM1721		MSDS	10
NoLimits™ 5000 bp DNA Fragment	SM1731		MSDS	10
NoLimits™ 6000 bp DNA Fragment	SM1511		MSDS	10
NoLimits™ 7000 bp DNA Fragment	SM1741		MSDS	10
NoLimits™ 8000 bp DNA Fragment	SM1521		MSDS	10
NoLimits™ 10000 bp DNA Fragment	SM1751		MSDS	10
NoLimits™ 15000 bp DNA Fragment	SM1531		MSDS	10
NoLimits™ 17000 bp DNA Fragment	SM1771		MSDS	10
NoLimits™ 20000 bp DNA Fragment	SM1541		MSDS	10

Concentration
0.5 µg/µl

Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) with 1 mM EDTA.

Quality Control
Tested on an Agilent 2100 bioanalyzer and by appropriate gel electrophoresis. DNA concentration determined spectrophotometrically. The absence of nucleases is confirmed by appropriate tests.

Storage
Store at -20°C.
 

Updated balandžio 24, 2008 18:35