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Frequently Asked Questions about Restriction Enzymes (8)

1. How should I store restriction enzymes?
   
   All restriction enzymes should be stored at -20°C. Keep restriction enzymes on ice while not in a freezer.
    
2. How should I dilute restriction enzyme?
   
   Use the Diluent Buffer for restriction enzymes, Cat# B19: 10 mM Tris-HCl, 100 mM KCl, 1 mM EDTA, 1 mM DTT, 50% glycerol, 200 µg/ml BSA (pH 7.4 at 25°C) to dilute all Fermentas restriction enzymes. Restriction enzymes with the same storage buffer composition (see the respective Certificate of Analysis) as that of the Diluent Buffer, retain their activity for at least 12 months after dilution in it. The restriction enzymes with different storage buffer requirements will retain more than 50% activity if stored at -20°C for 3-4 weeks after dilution with the Diluent Buffer.
    
3. Where and how long can I keep the restriction enzyme reaction buffers?
   
   For longer periods, we recommend to store the restriction enzyme buffers at -20°C.
   They can be stored at +4°C, but for no longer than 1 week in order to avoid possible microbiological contamination.

Updated gegužės 08, 2007 16:48