ProteoBlock™ Protease Inhibitor Cocktail

- Store at -20°C

Catalog#	Size, concentration	Supplied with:		Certificate of Analysis	MSDS
		100X ProteoBlock™ Protease Inhibitor Cocktail	0.5 M EDTA, pH 8.0
R1321		2 x 1.25 ml	3 x 1.00 ml	R1321

Product information

Features

Convenient – ready-to-use 100X solution.
Efficiently inhibits a broad range of proteases and esterases during protein extraction.
Flexible format – EDTA supplied in a separate vial.

Applications

Inhibition of proteolysis during protein extraction procedures from various cell or tissue samples for high yields of intact, functional proteins.

Description

Proteases are proteolytic enzymes that are present in all living cells and rapidly degrade proteins during extraction procedures. The resulting proteolytic damage significantly decreases the quality and yields of sample proteins. Addition of a protease inhibitor cocktail to the extraction buffer is highly recommended to protect proteins from degradation.

The ProteoBlock™ Protease Inhibitor Cocktail is a mixture of 6 different protease inhibitors. This ensures inhibition of a broad range of proteases and esterases (see Table 1 below). The protease inhibitor cocktail is supplied as a ready-to-use solution at a 100X concentration in DMSO. EDTA is provided in a separate tube for use in applications that are not inhibited by the presence of EDTA.

Sufficient ProteoBlock™ Protease Inhibitor Cocktail is supplied for preparation of 250 ml of extraction buffer.

Quality Control

The ProteoBlock™ Protease Inhibitor Cocktail is functionally tested in a validated spectrophotometric protease assay using pancreas extract.

Composition

100X ProteoBlock™ Protease Inhibitor Cocktail: 2 x 1.25 ml.
0.5 M EDTA, pH 8.0: 3 x 1 ml.

Storage

Store at -20°C.

Components of the ProteoBlock™ Protease Inhibitor Cocktail

Patents, Licenses, Trademarks

Protocols & recommendations

RECOMMENDATIONS FOR USE

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