Bradford Reagent, ready-to-use

- Store at 4°C

Catalog#	Size, concentration	Certificate of Analysis	MSDS

R1271	1 liter	R1271

Product information

Features

Ready-to-use formulation – no dilution is required.
Rapid color development.
Broad working concentration range – 1-2000 µg/ml.
Compatible with a variety of substances present in samples, including solvents, salts, detergents, thiols, reducing and chelating agents.

Applications

Rapid and accurate estimation of protein concentration in solution.

Description

Bradford Reagent, ready-to-use, is a convenient 1X concentrated dye reagent for rapid and accurate estimation of total protein concentration in solution.

The Bradford protein assay relies on the formation of complexes between Coomassie Brilliant Blue G-250 dye and proteins in solution. The binding of the dye to the protein causes a shift in the absorption maximum of the dye from 465 nm to 595 nm (1, 2). Unknown protein concentrations are determined by reference of their absorptions to absorptions obtained for series of standard protein dilutions assayed in parallel with the unknown samples.

The quantification procedure is simple and rapid. The dye binding process is complete in approximately 5 min with color stability for 1 hour.

The absorption is proportional to the amount of protein present in the solution. The working range is 1-2000 µg/ml of protein.

Depending on the protein concentration, standard or microassay procedures can be used. Depending on the volume of protein sample available, the assay can be performed in test tubes or microplates.

Quality Control

Functionally tested for accurate determination of various protein concentrations.

Storage

Store protected from light at 4°C for one year.

Standard curve obtained using Bradford reagent in standard assay

Figure 1. Standard curve obtained using Bradford reagent, ready-to-use, in standard assay.
BSA – bovine serum albumin
BGG – bovine gamma globulin

Standard curve obtained using Bradford reagent in micro assay

Figure 2. Standard curve obtained using Bradford reagent, ready-to-use, in micro assay.
BSA – bovine serum albumin
BGG – bovine gamma globulin

Patents, Licenses, Trademarks

Protocols & recommendations

RECOMMENDATIONS FOR USE

ADDITIONAL PROTOCOLS

Protein Samples: Extraction and Quantification Guides

Sample/procedure	Extraction	Quantification
Mammalian cell and tissue samples	Extract total proteins using ProteoJET™ Mammalian Cell Lysis Reagent. Extract nuclear and cytoplasmic proteins using ProteoJET™ Cytoplasmic and Nuclear Protein Extraction Kit.	Use Bradford Reagent, ready-to-use for protein quantification. Easily create standard curves by using one of our convenient protein standard sets: Bovine Serum Albumin Standard Set, Bovine Gamma Globulin Standard Set, Protein Standard Solution. Create a standard curve by plotting the 595 nm values (y-axis) versus their concentration in µg/ml (x-axis). Use the standard curve to determine the protein concentration of each unknown sample.
Bacterial samples	To analyze total bacterial proteins in SDS-PAGE, cells can be treated directly with DualColor™ Protein Loading Buffer Pack or Protein Loading Buffer Pack.
Lyophilized proteins	To analyze proteins in SDS-PAGE, treat lyophilized proteins directly with DualColor™ Protein Loading Buffer Pack or Protein Loading Buffer Pack.

References

Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, 248-54, 1976.
Sedmak, J.J., Grossberg, S.E., A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G-250, Anal. Biochem., 79, 544-52, 1977.

Related products