Standard PCR lacks specific requirements for template, fidelity, sensitivity and yield, template quality, sequence complexity. Standard PCR uses conventional Taq DNA polymerase or it's analogs to amplify targets from 100 bp to 3 kb long. After the completion of PCR, reaction products are analyzed by agarose gel electrophoresis and ethidium bromide staining. Quantification of final PCR product is possible by comparison with a known amount of similar size DNA fragment loaded in the same gel. PCR products can further be used for sequencing or cloning.