Sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in common cloning vectors, such as pTZ19R, pTZ57R, pBluescript II.
Description
Sequencing primers are single-stranded oligonucleotides with 5'- and 3'-hydroxyl ends. The primers are complementary to the SP6, T3 or T7 RNA polymerase promoter regions respectively. Primers are supplied as 10 µM aqueous solutions.
Quality Control
Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence.
Note
Primers cannot be used for certain plasmids that contain truncated, but still fully functional promoters.
- Worldwide
- to print
- Store at -20°C
