- Worldwide
Reverse transcription is a first step in two step RT-PCR. The RNA templates are reverse transcribed into cDNA which can then be amplified using PCR. The enzymes used are reverse transcriptases of different origins, most popular types are M-MuLV RT and AMV RT.
Table. Properties of reverse transcriptases.
Table. Properties of reverse transcriptases.
| Reverse transcriptase | Optimal reaction temperature | Active up to | Reading lenght | RNase H activity | Inactivation | Units/20 µl reaction | Amount of total RNA/20 µl reaction |
|---|---|---|---|---|---|---|---|
| Maxima™ Reverse Transcriptase | 50-55°C | 60°C | 20 kb | + | 85°C, 5 min | 200 u | 0.01 ng - 5 µg |
| RevertAid™ Premium Reverse Transcriptase | 50-55°C | 60°C | 20 kb | – | 85°C, 5 min | 200 u | 0.01 ng - 5 µg |
| RevertAid™ H Minus Reverse Transcriptase | 42-45°C | 55°C | 13 kb | – | 70°C, 10 min | 200 u | 0.1 ng - 5 µg |
| RevertAid™ Reverse Transcriptase | 42°C | 50°C | 13 kb | + | 70°C, 10 min | 200 u | 0.1 ng - 5 µg |
| M-MuLV Reverse Transcriptase | 37°C | 37°C | 9 kb | + | 70°C, 10 min | 40 u | 100 ng - 5 µg |
| AMV Reverse Transcriptase | 45-50°C | 60°C | 13 kb | ++ | 85°C, 5 min | 10 u | 10 ng - 5 µg |
- to print
- Reverse Transcription
- AMV Reverse Transcriptase
- First Strand cDNA Synthesis Kit
- M-MuLV Reverse Transcriptase
- Maxima™ First Strand cDNA Synthesis Kit for RT-qPCR new
- Maxima™ Reverse Transcriptase new
- Maxima™ Universal First Strand cDNA Synthesis Kit (for the USA and Canada only) new
- RevertAid™ First Strand cDNA Synthesis Kit
- RevertAid™ H Minus First Strand cDNA Synthesis Kit
- RevertAid™ H Minus Reverse Transcriptase
- RevertAid™ Premium First Strand cDNA Synthesis Kit new
- RevertAid™ Premium Reverse Transcriptase new
- RevertAid™ Reverse Transcriptase
- RiboLock™ RNase Inhibitor
